The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
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Dermal Bioactive Properties of the Ethanol Extract from Flowers of Lespedeza bicolor

싸리꽃 에탄올 추출물의 피부(皮膚) 생리활성(生理活性) 효과

  • 유인식 (원광대학교 한의학전문대학원) ;
  • 이성진 (원광대학교 한의학전문대학원) ;
  • 이성원 (원광대학교 한의학전문대학원) ;
  • 문연자 (원광대학교 한의학전문대학원) ;
  • 우원홍 (원광대학교 한의학전문대학원) ;
  • 김영목 (원광대학교 한의과대학 한방병리학교실) ;
  • 이장천 (상지대학교 한의과대학 본초방제학교실) ;
  • 임규상 (원광대학교 한의과대학 안이비인후피부과학교실)
  • Published : 2007.08.25
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Abstract

Objective : In this study, the ethanol extract from flowers of Lespedeza bicolor was investigated for their dermal bioactive properties related to cosmeceuticals such as depigmentation and radical scavenging effect. Results : The ethanol extract from flowers of Lespedeza bicolor showed considerable radical scavenging activity ($SC_{50}:\;10\;{\mu}g/ml$) and inhibited the production of nitric oxide (NO) in Raw 264.7 macrophages activated with the endotoxin lipopolysaccharide (LPS). Although the proliferation of B16/F10 cells was slightly decreased by the ethanol extract from flowers of Lespedeza bicolor at the concentration of $100\;{\mu}g/ml$, it did not appear necrosis. The ethanol extract from flowers of Lespedeza bicolor down-regulated melanin formation effectively. Methods : The free radical scavenging activity of Lespedeza bicolor was assayed in cell free systems using a stable free radical, 1,l-diphenyl-2-picrylhydrazyl (DPPH). Nitrite accumulated in culture medium was measured as an indicator of NO production using a Griess reaction. Cell viability was measured by MTT assay and melanin content was assessed using the method of Hosei with some modifications. Conclusions : These results suggest that the ethanol extract from flowers of Lespedeza bicolor is a potent depigmetation agent and it may be a candidate for antioxidant and anti-inflammatory agent.

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