The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Analysis of Marker Substances in Samul-tang by HPLC-MS/MS

HPLC-MS/MS에 의한 사물탕의 지표성분 분석

  • Yu, Young-Beob (Dept. of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine) ;
  • Kim, Mi-Jung (Dept. of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine) ;
  • Huang, Dae-Sun (Dept. of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine) ;
  • Ha, Hye-Kyeong (Dept. of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine) ;
  • Ma, Jin-Yeul (Dept. of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine) ;
  • Shin, Hyeun-Kyoo (Dept. of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine)
  • 유영법 (한국한의학연구원 한약제제연구부) ;
  • 김미정 (한국한의학연구원 한약제제연구부) ;
  • 황대선 (한국한의학연구원 한약제제연구부) ;
  • 하혜경 (한국한의학연구원 한약제제연구부) ;
  • 마진열 (한국한의학연구원 한약제제연구부) ;
  • 신현규 (한국한의학연구원 한약제제연구부)
  • Published : 2007.06.30
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Abstract

Objectives : This study presents a high performance liquid chromatography - electrospray ionization-mass spectrometer (HPLC-MS/MS) methods for the quantitative and qualitative analysis of various active components in Samul-tang, which is composed of four crude herbs. Methods : HPLC-ESI-MS/MS for the determinations of paeoniflorin and 5-HMF (5-hydroxymethyl 2-furaldehyde) in the Samul-tang, the separation method was performed on an COSMOS1L 5C18-AR-Il (2.0 X 150 mm I.D.) column by gradient elution with 0.1% acetic acid and 5% CH3CN in water (A)-0.1% acetic acid and 5% H20 in CH3CN (B) as the mobile phase at a flow-rate of 300 ${\mu}L/min$ with detection at quadrupole mass spectrometer. The all marker substances were always detected as the base peaks in the positive ion mode. Results : The paeoniflorin of Paeoniae Radix in Samul-tang showed a strong base peak [M+H2O]+ in the positive detection mode to give the following as; paeoniflorin (498.109 [M+H2O]+, 479.8 [M]+, 301 [M-glucose]+, 179.3 [glucose]+). Based on the HPLC retnetion time and MS of standard compounds confirmed the identity of active compounds in Rehmanniae Radix Preparata as follows; 5-HMF (127.0[M+H]+, 109.3 [M-OH]+) in the positive ion mode. Conclusion : According to the above results, HPLC-ESI-MS method permits assignment of tentative structures such as paeoniflorin and 5-HMF in the Samul-tang.

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