The Journal of Internal Korean Medicine (대한한방내과학회지)
- Volume 28 Issue 1
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- Pages.149-165
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- 2007
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- 1226-9174(pISSN)
The Effects of Artermisiae Capillaris Herba, Curcumae Rhizoma, Loranthi Ramulus, and Orostachys Herba on Expression of Angiogenic Factors in HepG2 Cells
인진(茵蔯), 울금(鬱金), 상기생(桑寄生), 와송(瓦松)이 HepG2 cell의 혈관생성인자 발현에 미치는 영향
- Kim, Ji-Kwon (Department of Internal Medicine, College of Oriental Medicine, Kyung Hee University) ;
- Kim, Young-Chul (Department of Internal Medicine, College of Oriental Medicine, Kyung Hee University) ;
-
Lee, Jang-Hoon
(Department of Internal Medicine, College of Oriental Medicine, Kyung Hee University)
;
- Woo, Hong-Jung (Department of Internal Medicine, College of Oriental Medicine, Kyung Hee University)
- 김지권 (경희대학교 한의과대학 간계내과학교실) ;
- 김영철 (경희대학교 한의과대학 간계내과학교실) ;
-
이장훈
(경희대학교 한의과대학 간계내과학교실)
;
- 우홍정 (경희대학교 한의과대학 간계내과학교실)
- Published : 2007.03.31
Abstract
Objectives : This study was designed to investigate the effects of Artermisiae Capillaris Herba, Curcumae Rhizoma, Loranthi Ramulus, and Orostachys Herba on expression of angiogenic factors in HepG2 cells. Materials and Methods : The mRNA expression level and protein secretion level of angiogenic factors were measured using quantitative RT-PCR, western blot and ELISA assay respectively in Artermisiae Capillaris Herba, Curcumae Rhizoma, Loranthi Ramulus, Orostachys Herba -treated and untreated HepG2 cells. Results : Curcumae Rhizoma, Loranthi Ramulus, and Orostachys Herba reduced mRNA expression level and protein secretion level of angiogenic factors, but Artermisiae Capillaris Herba increased it, especially VEGF and bFGF in HepG2 cells. Conclusions : The results indicate that Artermisiae Capillaris Herbapromotes expression of angiogenic factors but Curcumae Rhizoma, Loranthi Ramulus, and Orostachys Herba inhibit expression of angiogenic factors in HepG2 cells. The result is expected that Curcumae Rhizoma, Loranthi Ramulus, Orostachys Herba have an inhibitive effect of angiogenesis in HCC.