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Ecology of Crown Gall Disease and Population of Agrobacterium spp. in Vineyard Soils

포도나무 뿌리혹병 발생생태 및 포도 재배포장의 Agrobacterium 속의 밀도 조사

  • 최재을 (충남대학교 농업생명과학대학) ;
  • 강성수 (천안시농업기술센타) ;
  • 박상헌 (천안시농업기술센타) ;
  • 박문규 (천안시농업기술센타) ;
  • 박태진 (천안시농업기술센타) ;
  • 강희완 (한경대학교 생물환경정보통신전문대학원)
  • Published : 2008.08.01

Abstract

To investigate the severity of crown gall disease on grapevine, the ratio of healthy vs. galled grapevines and the presence of the pathogen of the disease in soil were measured in Korean vineyards. In field and greenhouse cultivations, the crown gall incidence of the cv. Kyoho grapevines was $0.4{\sim}97.9%$ and $1.4{\sim}3.8%$ and those of cv. Campbell Early was $1.2{\sim}2.1%$ and $0{\sim}1.8%$, respectively. The higher populations of Agrobacterium spp. were isolated from soils of grapeveins with crown gall than from soils of noninfected vineyards. Based on the colony shapes and growth on plates, 480 isolates of Agrobacterium spp. from 21 soil samples were collected. Only 13 isolates out of 480 developed the gall on inoculated grapevines.

한국 포도나무의 뿌리혹병에 의한 발생생태를 조사하기 위하여 뿌리혹병이 발병주율 및 포도나무 재배포장에서 병원균을 측정하였다. 노지와 하우스재배 '거봉'의 뿌리혹병 발병주율은 각각 $0.4{\sim}97.9%$$1.4{\sim}3.8%$, '캠벨얼리'의 발병주율은 각각 $1.2{\sim}2.1%$$0{\sim}1.8%$이었다. Agrobacterium 속의 밀도는 뿌리혹병이 발생한 포장의 토양이 뿌리혹병이 발생하지 않은 토양보다 많이 분리되었다. 플레이트상에서 콜로니 형태와 크기로 21개의 포도나무 토양 샘플로부터 Agrobacterium spp. 480균주를 선발하였다. 이들 중 포도나무에 접종하였을 때 13균주만이 뿌리혹을 형성하였다.

Keywords

References

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