Journal of Oriental Neuropsychiatry (동의신경정신과학회지)

The Korean Society of Oriental Neuropsychiatry (대한한방신경정신과학회)
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The Effects of Antioxidant and Anti-Alzheimer on Hydrogen peroxide and $\beta$-amyloid peptid-induced PC 12 cells by Semen Ziziphi Spinosae water extract

$H_{2}O_2$와 A$\beta$로 유도된 pc12 cell에서 생산조인(生酸棗仁) 수추출물의 항산화 및 항치매 효과

  • Lee, Sang-Won (Dept. of neuropsychiatry, college of Oriental Medicine, WonKwang University) ;
  • Kim, Dae-Hyun (Dept. of neuropsychiatry, college of Oriental Medicine, WonKwang University) ;
  • Yun, Jong-Hyun (Dept. of neuropsychiatry, college of Oriental Medicine, WonKwang University) ;
  • Kim, Jin-Woo (Dept. of neuropsychiatry, college of Oriental Medicine, WonKwang University) ;
  • Jung, Ejun-Young (Dept. of neuropsychiatry, college of Oriental Medicine, WonKwang University) ;
  • Lee, Seoung-Geun (Dept. of internal medicine, college of Oriental Medicine, WonKwang University) ;
  • Lee, Key-Sang (Dept. of internal medicine, college of Oriental Medicine, WonKwang University) ;
  • Kim, Tae-Heon (Dept. of neuropsychiatry, college of Oriental Medicine, WonKwang University) ;
  • Lyu, Yeoung-Su (Dept. of neuropsychiatry, college of Oriental Medicine, WonKwang University) ;
  • Kang, Hyung-Won (Department of Neuropsychiatry and Inam Neuroscience Research Center, WonKwang University, San bon Oriental Medical Center)
  • 이상원 (원광대학교 한의과대학 한방신경정신과학교실) ;
  • 김대현 (원광대학교 한의과대학 한방신경정신과학교실) ;
  • 윤종현 (원광대학교 한의과대학 한방신경정신과학교실) ;
  • 김진우 (원광대학교 한의과대학 한방신경정신과학교실) ;
  • 정은영 (원광대학교 한의과대학 한방신경정신과학교실) ;
  • 이성근 (원광대학교 한의과대학 한방내과학교실) ;
  • 이기상 (원광대학교 한의과대학 한방내과학교실) ;
  • 김태헌 (원광대학교 한의과대학 한방신경정신과학교실) ;
  • 류영수 (원광대학교 한의과대학 한방신경정신과학교실) ;
  • 강형원 (원광대학교 산본한방병원 신경정신과 & 인암뇌신경센타)
  • Published : 2008.11.30
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Abstract

Objective: The antioxidant and anti-Alzheimer effects of Semen Ziziphi Spinosae (SZS) water extract against the amyloid beta peptide (1-42) or H202-induced oxidative damage and cell death were investigated in rat pheochromocytoma line PC 12. Methods: The cells were incubated with SZS water extract and oxidative damage-inducing materials, amyloid beta peptide (1-42) or H2O2 for 24 h. The cellular viability was assessed by WST-1 assay, cytotoxic damage by LDH activity assay, oxidative damages of cells by fluorescence spectrophotometric method, and apoptosis by TUNEL staining assay. Results and Conclusions: 1. Preincubation of the cells with SZS water extract prior to amyloid beta peptide (1-42) (2 uM) or H2O2 (30 uM) exposure elevated the cell survival close to the control and decreased the level of LDH activity and the fluorescence from the cell homogenates and TUNEL staining of the cells, compared to only amyloid beta peptide (1-42) (2 uM) or H2O2 (30 uM) treated conditions. 2. Our study suggests that Semen Ziziphi Spinosae (SZS) water extract has protective effects against amyloid beta peptide (1-42) or H2O2-induced cell toxicity through the antioxidation mechanism, which might be beneficial for the treatment of Alzheimer's disease.

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