Journal of the korean academy of Pediatric Dentistry (대한소아치과학회지)

Korean Academy of Pediatric Dentistry (대한소아치과학회)
권호 표지

The Relationship between the Salivary IgA against AgI/II of S. mutans and Dental Caries Experience among Children and Adults

소아와 성인의 타액 내 AgI/II 특이 IgA 와 우식경험도의 관계

  • Lim, Su-Min (Department of Pediatric Dentistry and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University) ;
  • Kim, Jae-Gon (Department of Pediatric Dentistry and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University) ;
  • Baik, Byeong-Ju (Department of Pediatric Dentistry and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University) ;
  • Yang, Yeon-Mi (Department of Pediatric Dentistry and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University) ;
  • Kim, Su-Kyung (Department of Pediatric Dentistry and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University) ;
  • Lee, Kyung-Yol (Department of Pediatric Dentistry and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University)
  • 임수민 (전북대학교 치과대학 소아치과학교실 및 구강생체과학연구소) ;
  • 김재곤 (전북대학교 치과대학 소아치과학교실 및 구강생체과학연구소) ;
  • 백병주 (전북대학교 치과대학 소아치과학교실 및 구강생체과학연구소) ;
  • 양연미 (전북대학교 치과대학 소아치과학교실 및 구강생체과학연구소) ;
  • 김수경 (전북대학교 치과대학 소아치과학교실 및 구강생체과학연구소) ;
  • 이경열 (전북대학교 치과대학 소아치과학교실 및 구강생체과학연구소)
  • Published : 2008.11.30
Download PDF
⟨ Previous Next ⟩

Abstract

AgI/II of Streptococcus mutans(S. mutans) is an important virulence factor that contributes to the pathogenesis of S. mutans-induced dental caries. In oral cavity, salivary IgA antibodies act as safeguards against enormous challenges from oral bacteria. IgA antibodies inhibit adherence of cariogenic microorganisms to hard surfaces. Analysis of salivary IgA against AgI/II can be very useful diagnostic and powerful communication tools to the dental caries The purpose of this study was to investigate correlation between salivary AgI/II specific IgA and incidence of dental caries among children and young adults. Subjects consisted of 28 children and 18 adults. They were assigned to four groups : Group I deft index $\leq$3), Group II(deft index $\geq$4), Group III(DMFT index $\leq$3), Group IV(DMFT index $\geq$4) and they was divided two groups into caries resistant group and caries susceptible group. The study group were examined caries activity and their salivary IgA was evaluated by enzyme-linked immunosorbent assay. The results are as follows : 1. There was a positive correlation between the number of S. mutans and caries activity. 2. The titer of salivary IgA against the AgI/II was significantly higher in caries resistant group than caries susceptible group(p<0.01). 3. The titer of salivary IgA against the AgI/II in Group III was significantly higher than Group II(p<0.05).

치아 우식증은 감염성 질환의 하나로 치아우식의 원인균은 Streptococcus mutans(S. mutans)와 같은 mutans steptococci로 알려져 있다. S. mutans가 치면에 접착하여 군집을 형성하는 능력은 균독성에 중요한 역할을 하는데, AgI/II와 같은 세포 표면의 섬유성 단백질을 매개로 한다. Secretory IgA는 타액이나 누.비액, 초유, 그리고 폐나 소화기관의 분비액에서 선택적으로 다량 발견되는데 타액에서 secretory IgA는 S. mutans의 대사활동을 억제하고 치면으로의 부착을 방해한다. 이전의 몇몇 연구에서 S. mutans에 특이적인 타액 내 IgA와 우식경험도는 역상관관계를 보인다고 발표하였다. 그러나 다른 연구에서 통계적 유의성이 없다고 보고하기도 하였다. 본 연구의 목적은 소아, 성인의 치아우식증과 S. mutans의 AgI/II에 특이적인 타액 내 IgA와의 관계를 알기위한 것이다. 이를 위해 소아(평균6세) 28명, 성인(20-30세) 18명을 대상으로 Group Ⅰ(deft index ${\leq}$ 3), Group II(deft index ${\geq}$ 4), Group III(DMFT index ${\leq}$ 3), Group IV(DMFT index ${\geq}$ 4)로 분류하였다. 그리고 caries resistant group(CR group, deft or DMFT index ${\leq}$ 3)과 caries susceptible group(CS group, deft or DMFT index ${\geq}$ 4)으로 분류하였다. S. mutans 수와 우식경험도 간에는 통계적으로 유의한 상관관계를 나타냈다. AgI/II 특이 salivary IgA titer는 Group III이 Group II보다 통계적으로 유의하게 더 컸으며, CR group이 CS group보다 유의하게 크게 나타났다.

Keywords

References

  1. Michalek SM, Childers NK : Development and outlook for a caries vaccine. Crit Rev Oral Biol Med, 1:37-54, 1990.
  2. Loesche WJ, Rowan LH, Staraffon LH, et al. : Association of Streptococcus mutans with human dental decay. Infec Immun, 11:1253-1260, 1975.
  3. Losesche WJ, Straffon LH : Longitudinal investigation of the role of Streptococcus mutans in tissue decay. Infec Immun, 26:498-507, 1979.
  4. Loesche WJ : Role of Streptococcus mutans in human dental decay. Microbiol Rev, 50:353-380, 1986.
  5. Kuramitsu HK : Virulence factors of mutans streptococci ; role of molecular genetics. Crit Rev Oral Biol Med, 4:159-176, 1993.
  6. Russell MW, Lehner T : Characterization of antigens extracted from cells and culture fluid of Streptococcus mutans serotype c. Arch Oral Biol, 23:7-15, 1978. https://doi.org/10.1016/0003-9969(78)90047-X
  7. Russell MW, Bergmeier LA, Zanders ED, et al. : Protein antigen of Streptococcus mutans ; purification and properties of a double antigen and its protease-resistant component. Infect Immun, 28:486-493, 1980.
  8. Forester H, Hunter N, Knox KW : Characteristics of a high molecular weight extracellular protein of Streptococcus mutans. J Gen Microbiol, 129:2779-2788, 1983.
  9. Ji-Hye Han, Jae-Gon Kim, Byeong-Ju Baik, et al. : Generation of antibodies against N-terminus fragment of AgI/II protein from Streptococcus mutans GS-5. J KAPD, 33:515-519, 2006.
  10. Saito M, Otake S, Ohmura M, et al. : Protective immunity to Streptococcus mutans induced by nasal vaccination with surface protein antigen and mutant cholera toxin adjuvant. J Infect Dis, 183:823-826, 2001. https://doi.org/10.1086/318826
  11. Russel MW, Mansson-Rahemtulla B : Interaction between surface protein antigens of Streptococcus mutans and human salivary components. Oral Microbiol Immunol, 4:106-111, 1989. https://doi.org/10.1111/j.1399-302X.1989.tb00107.x
  12. Koga T, Okahashi N, Takahashi I, et al. : Surface hydrophobicity, adherence, and aggregation of cell surface protein antigen mutans of Streptococcus mutans serotype c. Infect Immun, 58:289-296, 1990.
  13. Lee SF, Progulske-Fox A, Erdos GW, et al.: Construction and characterization of isogenic mutants of Streptococcus mutans deficient in major surface protein antigen P1(AgI/II). Infect Immun, 57:3306-3313, 1989.
  14. Bowen WH, Schilling K, Giertsen E, et al. : Role of a cell surface associated protein in adherence and dental caries. Infect Immun, 59:4406-4609, 1991.
  15. Schilling KM, Bowen WH : Glucans synthesized in situ in experimental salivary pellicle function as specific binding sites for Streptococcus mutans. Infect Immun, 60:284-295, 1992.
  16. Olson GA, Bleiweis AS, Small PA : Adherence inhibition of Streptococcus mutans ; An assay reflection a possible role of antibody in dental caries prophylaxis. Infect Immun, 5:419-427, 1972.
  17. Evans RT, Genco RJ : Inhibition of glucosyltransferase activity by antisera to known serotypes of Streptococcus mutans. Infect Immun, 7:237-241, 1973.
  18. Ogra PL, Mestecky J, Lamm ME, et al. : Handbook of mucosal immunology. Academic Press, 127-137, 1994.
  19. Camling E, Kohler B : Infection with the bacterium Streptococcus mutans and salivary IgA antibodies in mothers and their children. Arch Oral Biol, 32:817-23, 1987. https://doi.org/10.1016/0003-9969(87)90009-4
  20. Chalocombe SJ : Serum and salivary antibodies to Streptococcus mutans in relation to the development and treatment of human dental caries. Arch Oral Biol, 25:495-502, 1980. https://doi.org/10.1016/0003-9969(80)90058-8
  21. Tenovuo J, Laine M, Lehtonen O-PJ : Salivary IgA antibodies reaction with Streptococcus mutans in relation to changes in salivary S. mutans counts ; a longitudinal study in humans. Proc Finn Dent Soc, 83:55-59, 1987.
  22. Bwen WH : A vaccine against dental caries. A pilot experiment in monkeys. Br Dent J, 126:159-160, 1969.
  23. Lehner T, Challacombe SJ, Caldwell J : Immunological and bacteriological basis for vaccination against dental caries in rhesus monkeys. Nature, 254:517-520, 1975. https://doi.org/10.1038/254517a0
  24. McGhee JR, Michalek SM, Webb J, et al. : Effective immunity to dental caries ; protection of gnotobiotic rats by local immunization with Streptococcus mutans. J Immunol, 114:300-305, 1975.
  25. Takada K, Hirasawa M : A novel selective medium for isolation of Streptococcus mutans. J Microbiol Method, 60:189-193, 2005. https://doi.org/10.1016/j.mimet.2004.09.009
  26. Fan MW, Bian ZX, Peng Y, et al : A DNA vaccine encoding a cell-surface protein antigen of Streptococcus mutans protects gnotobiotic rats from caries. J Dent Res, 81:784-787, 2002. https://doi.org/10.1177/154405910208101112
  27. Naspitx GMCC, Nagao AT, Mayer MPA : Anti-Streptococcus mutans antibodies in saliva of children with different degrees of dental caries. Pediatr Allergy Immunol, 10:143-148, 1999. https://doi.org/10.1034/j.1399-3038.1999.00026.x