KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Melanin Inhibitory Effect and Anti-inflammatory Effects of Dietyota coriacea Extracts Derived from Adjacent Sea of the Jeju Island

제주도 근해에 자생하는 참가죽그물바탕말 [Dictyota coriacea] 추출물의 멜라닌 억제 효과 및 항염증 효과

  • Kang, Min-Chul (Jeju Bio-Industry Development Center, Jeju Hi-Tech Industry Development Institute) ;
  • Lee, Ju-Yeop (Jeju Bio-Industry Development Center, Jeju Hi-Tech Industry Development Institute) ;
  • Ko, Ryeo-Kyeoung (Jeju Bio-Industry Development Center, Jeju Hi-Tech Industry Development Institute) ;
  • Kim, Haeng-Bum (Jeju Bio-Industry Development Center, Jeju Hi-Tech Industry Development Institute) ;
  • Hong, Seung-Ho (Department of Science Education, Teachers College, Cheju National University) ;
  • Kim, Gi-Ok (Jeju Bio-Industry Development Center, Jeju Hi-Tech Industry Development Institute)
  • 강민철 ((재)제주하이테크산업진흥원 생물자원산업화지원센터) ;
  • 이주엽 ((재)제주하이테크산업진흥원 생물자원산업화지원센터) ;
  • 고려경 ((재)제주하이테크산업진흥원 생물자원산업화지원센터) ;
  • 김행범 ((재)제주하이테크산업진흥원 생물자원산업화지원센터) ;
  • 홍승호 (제주대학교 교육대학 과학교육과) ;
  • 김기옥 ((재)제주하이테크산업진흥원 생물자원산업화지원센터)
  • Published : 2008.08.29
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Abstract

We investigated several biological activities using the ethanol extract and its fractions from Dictyota coriacea to evaluate the usefulness of its extract as a functional biomaterial. The ethanol extract and n-hexane and ethyl acetate fractions showed dependently inhibitory effect on tyrosinase activity and melanin content in B16F10 cells. The ethanol extract and its fractions showed inhibitory effect on Tyrosinase and TRP-1 gene transcription but didn't showed inhibitory effect on TRP-2 gene transcription. Also, the n-hexane and ethyl acetate fractions dose-dependently inhibited the NO production in a RAW 264.7 cells. These results suggest that extract of Dictyota coriacea could be used as functional biomaterial in developing a skin whitening agent having the anti-inflammatory activity.

본 연구는 제주도 근해에 자생하는 참가죽그물바탕말 (Dictyota coriacea)의 에탄올 추출물 및 순차적 용매분획물을 얻어 B16F10 mouse melanoma 세포및 RAW 264.7 세포에 처리하여 멜라닌 생합성 및 tyrosinas 억제활성, NO 억제활성을 통하여 유용자원으로서 미백 및 기능성 화장료로 산업적 활용 가능성이 있는지 알아보고자 연구를 시행하였다. 실험한 결과 B16F10 세포에서 참가죽그물바탕말 에탄올 추출물과 헥산 분획물, 에틸아세테이트 분획물이 tyrosinase와 멜라닌 합성을 농도 의존적으로 뛰어나게 억제하였으며, 단백질 및 mRNA 발현양이 tyrosinase와 TRP-1 유전자에서 농도 의존적으로 감소함을 보였다. 하지만 TRP-2 단백질과 mRNA 유전자의 발현을 확인한 결과, TRP-2 단백질과 mRNA 발현이 농도에 관계없이 일정하였다. 또한 LPS로 자극한 RAW 264.7 세포에서 참가죽그물바탕말의 에탄을 추출물과 헥산 분획물, 에틸아세테이트 분획물이 가장 높은 NO 생성 억제 활성을 보였다. 따라서 본 연구결과는 참가죽그물바탕말 추출물이 항염증 활성을 가지는 미백 관련 기능성 소재로서의 활용가치가 있음을 보여준다.

Keywords

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