The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Effect of Heavy Metals on Mycelial Growth of Color Mutants at Pleurotus ostreatus

중금속이 느타리버섯 자실체 색변이체의 균사생장에 미치는 영향

  • Lee, Kang-Hyo (Mushroom Research Division, National Academy of Agricultural Science, RDA) ;
  • Seok, Soon-Ja (National Agrobiodiversity Center, National Academy of Agricultural Science, RDA) ;
  • Weon, Hang-Yeon (Mushroom Research Division, National Academy of Agricultural Science, RDA) ;
  • Kim, Seung-Hwan (Mushroom Research Division, National Academy of Agricultural Science, RDA) ;
  • Kim, Wan-Gyu (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA) ;
  • Sung, Jae-Mo (Department of Agricultural Biology, Kangwon National University)
  • 이강효 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 석순자 (농촌진흥청 국립농업과학원 유전자원센터) ;
  • 원항연 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 김승환 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 김완규 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 성재모 (강원대학교 농업생명과학대 생물환경학부)
  • Published : 2008.12.31
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Abstract

Mycelial growth of Pleurotus ostreatus isolates was examined on PDA media added with heavy metals to reveal effects of heavy metals on mycelial growth of the fungus. Cd and Cu strongly inhibited mycelial growth of three isolates of fungus tested. However, addition of $2{\sim}10\;mM$ Pb to growing media of white color mutant of fungus resulted in increase of the fungal growth rate. Addition of 2 mM Cr to the media resulted in increase of growth rate of the white color mutant and the dark color mutant of fungus. Mycelial growth rate of the white color mutant was relatively better than the other isolates on media added with Cr, Pb, Cu, or Mn, respectly. Tolerance of the white color mutans to heavy metals was higher than that of the black color mutant. It is suggested that tolerance of the white color mutant to heavy metals was not controled by color-related substance of the fungus.

느타리버섯(Pleurotus ostreatus)의 자실체색 돌연변이체를 중금속이 첨가된 배지에서 배양한 결과, Cd과 Cu는 균사생장을 크게 저하시켰다. 그러나 $2{\sim}10\;mM$의 Pb가 첨가된 배지에서는 백색 변이체(MGL2205)의 균사생장이 촉진되었으며, 2 mM의 Cr 첨가배지에서는 백색 변이체(MGL2205)와 진회색 변이체(MGL2308)의 균사생장이 촉진되었다. 백색 변이체(MGL2205)는 다른 균주에 비해 Cr, Pb, Cu, Mn 등 중금속이 첨가된 배지에서 상대적으로 균사생장이 촉진되었다. 또한 중금속에 대한 백색 변이체(MGL2205)의 내성도 다른 균주들에 비해 높은 것으로 확인되었다. 따라서 백색 변이체의 중금속에 대한 내성은 자실체색 관련 대사경로와는 다른 기작에 의해 영향을 받는 것으로 보인다.

Keywords

References

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