Evaluation of the Efficacy of Sanitizers on Food Contact Surfaces Using a Surface Test Method

표면시험법을 이용한 식품접촉표면 재질에 따른 살균소독제의 유효성 평가

  • Kim, Hyung-Il (Food Packaging Division, Korea Food and Drug Administration) ;
  • Jeon, Dae-Hoon (Food Packaging Division, Korea Food and Drug Administration) ;
  • Yoon, Hae-Jung (Food Packaging Division, Korea Food and Drug Administration) ;
  • Choi, Hyun-Cheol (Food Packaging Division, Korea Food and Drug Administration) ;
  • Eom, Mi-Ok (Food Packaging Division, Korea Food and Drug Administration) ;
  • Sung, Jun-Hyun (Food Packaging Division, Korea Food and Drug Administration) ;
  • Park, Na-Young (Food Packaging Division, Korea Food and Drug Administration) ;
  • Won, Sun-Ah (Food Packaging Division, Korea Food and Drug Administration) ;
  • Kim, Nan-Young (Food Packaging Division, Korea Food and Drug Administration) ;
  • Lee, Young-Ja (Food Packaging Division, Korea Food and Drug Administration)
  • 김형일 (식품의약품안전청 용기포장과) ;
  • 전대훈 (식품의약품안전청 용기포장과) ;
  • 윤혜정 (식품의약품안전청 용기포장과) ;
  • 최현철 (식품의약품안전청 용기포장과) ;
  • 엄미옥 (식품의약품안전청 용기포장과) ;
  • 성준현 (식품의약품안전청 용기포장과) ;
  • 박나영 (식품의약품안전청 용기포장과) ;
  • 원선아 (식품의약품안전청 용기포장과) ;
  • 김난영 (식품의약품안전청 용기포장과) ;
  • 이영자 (식품의약품안전청 용기포장과)
  • Published : 2008.12.31

Abstract

The study was undertaken to provide information on the efficacy of sanitizers against bacteria with and without organic road dried on to food contact surfaces using the surface test method which EU and USA are currently implementing as one of their official test methods. Escherochia coli ATCC 10536 or Staphylococcus aureus ATCC 6538 was inoculated on to food contact surfaces, such as stainless steel, polypropylene, and silicon, which was then treated with benzalkonium chloride, sodium hypochlorite, or ethanol as a sanitizer for 5minutes at $20^{\circ}C$. Results indicated that the type of surface had little affected the efficacy of various sanitizers. In addition, 200 ppm of benzalkonium chloride or 200 ppm of sodium hypochlorite showed no definite reduction of bacterial populations in the present of organic load, while 40% ethanol showed reduction to $4\;cfu\;\log_{10}$/carrier or more in viable count in the organic load.

현재 미국 및 유럽에서 공정시험법 중 하나로 인정되고 있는 표면시험법을 사용하여 유기물과 함께 또는 유기물 없이 세균만 건조된 식품접촉 표면에서의 살균소독제 유효성에 대한 정보를 제공하고자 식품접촉표면으로 사용되는 스테인리스, 폴리프로필렌 및 실리콘에 Escherochia coli ATCC 10536 또는 Staphylococcus aureus ATCC 6538을 접종하고 살균소독제로서 염화벤잘코늄, 차아염소산나트륨 또는 에탄올을 $20^{\circ}C$에서 5분간 처리하였다. 그 결과, 각 표면의 종류는 살균소독제의 유효성에 큰 영향을 미치지 않았으며, 200 ppm 농도의 염화벤잘코늄 및 차아염소산나트륨은 유기물질이 존재할 경우 생균수를 $4\;cfu\;\log_{10}$/carrier 이상 감소시키지 못하였으나, 40% 에탄올은 생균수를 $4\;cfu\;\log_{10}$/carrier 이상 감소시키는 것으로 나타났다.

Keywords

References

  1. 김형일, 이광호, 곽인신, 엄미옥, 전대훈, 성준현, 최정미, 강한샘, 김용수, 강길진: 살균소독력 시험법 확립 및 살균소독력 평가, 한국식품과학회지, 37(5), 838-843 (2005)
  2. 김형일, 전대훈, 강길진, 엄미옥, 성준현, 강한샘, 곽효선, 권기성, 이영자: 유통식품에서 분리한 Staphylococcus aureus 및 Escherichia coli의 살균소독제 감수성, 한국식품과학회지, 39(1), 104-107 (2007)
  3. 김형일, 윤혜정, 최현철, 전대훈, 엄미옥, 성준현, 박나영, 원선아, 김남희, 성덕화, 곽효선, 권기성, 이영자: Listeria monocytogenes와 Salmonella spp.에 대한 Sodium Hypochlorite 및 Benzalkonium Chloride의 유효성 평가, 한국식품위생안전성학회지, 22(2), 132-136 (2007)
  4. 김일진, 김용수, 김형일, 최현철, 전대훈, 이영자, 하상도: Vibrio parahaemolyticus 표준 및 식품분리주에 대한 살균소독제 유효성분별 감수성 평가, 한국식품위생안전성학회지, 22(2), 127-131 (2007)
  5. Kusumaningrum, H.D., Riboldi, G., Hazeleger, W.C., and Beumer, R.R.: Survival of foodborne pathogens on stainless steel surfaces and cross-contamination to foods. Int. J. Food Microbiol., 85, 227-236 (2003) https://doi.org/10.1016/S0168-1605(02)00540-8
  6. Fuster-Valls, N., Hernandez-Herrero, M., Marin-de-Mateo, M., and Rodriguez-Jerez, J.J.: Effect of different environmental condotion on the bacteria survival on stainless steel surfaces. Food Control, 19, 308-314 (2008) https://doi.org/10.1016/j.foodcont.2007.04.013
  7. Gibson, H., Elton, R., Peters, W., and Holah, J. T.: Surface and suspension testing: Conflict or complementary. Int. Biodeter Biodegr, 36, 375-384 (1995) https://doi.org/10.1016/0964-8305(95)00096-8
  8. Peng, J.S., Tsai, W.C., and Chou, C.C.: Inactivation and removal of Bacillus cereus by sanitizer and detergent. Int. J. Food Microbiol., 77, 11-18 (2002) https://doi.org/10.1016/S0168-1605(02)00060-0
  9. Chemical disinfectants and antiseptics-Quantitative nonporous surface test of bactericidal and/or fungicidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas-Test method and requirements(phase 2/step 2). European committee for standardization, EN 13697, British standards institution(2001)
  10. American Society for Testing and Materials : Standard quantitative carrier test method to evaluate the bactericidal, fungicidal, mycobactericidal and sporicidal potencies of liquid chemical germicides(Designation : E 2111-00), ASTM International, West conshohocken, PA, USA (2001)
  11. American Society for Testing and Materials : Standard quantitative disk carrier test method for determining the bactericidal, virucidal, fungicidal, mycobactericidal and sporicidal activities of liquid chemical germicides(Designation : E 2197-02), ASTM International, West conshohocken, PA, USA (2002)
  12. Ronner, A.B. and Wong, A.C.L.: Biofilm development and sanitizer inactivation of Listeria monocytogenes and Salmonella typhimurium on stainless steel and Buna-n rubber. J. Food Prot, 56(9), 750-758 (1993) https://doi.org/10.4315/0362-028X-56.9.750
  13. Frank, J.F. and Chmielewski, R.A.N.: Effectiveness of sanitation with quaternary ammonium compound or chlorine on stainless steel and other domestic food-preparation surfaces. J. Food Prot, 60(1), 43-47 (1997) https://doi.org/10.4315/0362-028X-60.1.43
  14. Mafu, A.A., Roy, D., Goulet, J., and Magny, P.: Attachment of Listeria monocytogenes to stainless steel, glass, polypropylene, and rubber surfaces after short contact times. J. Food Prot, 53(9), 742-746 (1990) https://doi.org/10.4315/0362-028X-53.9.742
  15. Oulahal, N., Brice, W., Martial, A., and Degraeve, P.: Quantitative analysis of survival of Staphylococcus aureus or Listeria innocua on two types of surfaces: Polypropylene and stainless steel in contact with three different dairy products. Food Control, 19, 178-185 (2008) https://doi.org/10.1016/j.foodcont.2007.03.006
  16. Klingeren, B.V.: Disinfectant testing on surfaces. J. Hosp. Infect., 30(Supplement), 397-408 (1995) https://doi.org/10.1016/0195-6701(95)90044-6
  17. Bloomfield, S.: Chlorine and iodine formulations. In Handbook of disinfectants and antiseptics. (Joseph A. ed) Marcel Dekker, Inc., New York, pp. 133-158 (1996)
  18. Lopes, J.A.: Evaluation of dairy and food plant sanitizers against Salmonella typhimurium and Listeria monocytogenes. J. Dairy Sci., 69, 2791-2796 (1986) https://doi.org/10.3168/jds.S0022-0302(86)80731-7
  19. Bloomfield, S.F., Arthur, M., Klingeren B.V., Pullen, W., Holah, J.T., and Eltom, R.: An evaluation of the repeatability and reproducibility of a surface test for the activity of disinfectants. J. Appl. Bactriol., 76, 86-94 (1994) https://doi.org/10.1111/j.1365-2672.1994.tb04420.x