Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Buddleja officinalis prevents the normal cells from oxidative damage via antioxidant activity

  • Published : 2008.12.31
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Abstract

The flowers of Buddleja officinalis are used to treat sore and damaged eyes, a condition which is similar to skin wounds. However, whether it has any protective effect on oxidative DNA damage and cell death induced by hydroxyl radical remains unclear. In this study, we evaluated the protective effects of the extracts against oxidative DNA and cell damage caused by hydroxyl radical. DPPH radical, hydroxyl radical, hydrogen peroxide and intracellular ROS scavenging assay, and $Fe^{2+}$ chelating assay were used to evaluate the antioxidant properties. phi X 174 RF I plasmid DNA and intracellular DNA migration assay were used to evaluate the protective effect against oxidative DNA damage. Lastly, MTT assay and lipid peroxidation assay were used to evaluate the protective effect against oxidative cell damage. It was found to prevent intracellular DNA and the normal cells from oxidative damage caused by hydroxyl radical via antioxidant activities. These results suggest that Buddleja officinalis may exert the inhibitory effect on ROS-induced carcinogenesis by blocking oxidative DNA damage and cell death.

Keywords

References

  1. Armitage P., Doll, R., 1954. The age distribution of cancer and amulti-stage theory of carcinogenesis. Br. J. Cancer. 8: 1-12 https://doi.org/10.1038/bjc.1954.1
  2. Barja, G., 2004. Free radicals and aging, Trends Neurosci. 27: 595-600 https://doi.org/10.1016/j.tins.2004.07.005
  3. Barreto, R., Kawakita, S., Tsuchiya, J.,Minelli, E., Pavasuthipasit, K., Helmy, A., Marotta, F., 2005. Metal-induced oxidative damage in cultured hepatocytes and hepatic lysosomal fraction: beneficial effect of a curcumin/absinthium compound. Chinese Journal of Digestive Diseases. 6: 31-36 https://doi.org/10.1111/j.1443-9573.2005.00184.x
  4. Chaudhary, A.K., Nokubo, M., Reddy, G.R., Yeola, S.N., Morrow, J.D., Blair, I.A., Marnett, L.J., 1994. Detection of endogenous malondialdehyde-deoxyguanosine adducts in human liver. Science. 265; 1580-1582 https://doi.org/10.1126/science.8079172
  5. Esterbauer, H., 1982. In: McBrien, D.C.H., Slater, T.F. (Eds), Free Radicals, Lipid Peroxidation and Cancer. Academic Press, New York, pp 101-128
  6. Ghosal, D., Omelchenko,M.V., Gaidamakova, E.K.,Matrosova, V.Y., Vasilenko, A., Venkateswaran, A., Zhai, M., Kostandarithes, H.M., Brim, H., Makarova, K.S., Wackett, L.P., Fredrickson, J.K.,Daly,M.J., 2005. Howradiation kills cells: survival of Deinococcus radiodurans and Shewanella oneidensis under oxidative stress. FEMS Microbiol. Rev. 29: 361-375 https://doi.org/10.1016/j.femsre.2004.12.007
  7. Grisham,M.B., 1992. Reactive oxygenmetabolism. In: Grisham M.B., ed. Reactive metabolites of oxygen and nitrogen in biology and medicine. Austin: RG Landers Company. pp 39
  8. Houghton, P.J., 1984. Ethnopharmacology of some Buddleja species, Journal of Ethnopharmacology. 11: 293-308 https://doi.org/10.1016/0378-8741(84)90075-8
  9. Hus, B., Coupar, I.M., Ng, K., 2006. Antioxidant activity of hot water extract from the fruit of the Doum palm. Hyphaene thebaica. Food Chem. 98: 317-328 https://doi.org/10.1016/j.foodchem.2005.05.077
  10. Jung, Y, Surh, Y, 2001. Oxidative DNAdamage and cytotoxicity unduced by copper-stimulated redox cycling of salsolinol, a neurotoxic tetrahydroisoquinoline alkaloid. Free Radic. Biol. Med. 30: 1407-1417 https://doi.org/10.1016/S0891-5849(01)00548-2
  11. Kang, K.A., Zhang, R., Piao,M.J., Ko, D.O.,Wang, Z.H., Kim, B.J., Park, J.W., Kim, H.S., Kim, D.H., Hyun, J.W., 2008. Protective effect of irisolidone, a metabolite of kakkalide, against hydrogen peroxide induced cell damage via antioxidant effect. Bioorganic & Medicinal Chemistry. 16; 1133-1141 https://doi.org/10.1016/j.bmc.2007.10.085
  12. Leonard, S.S., Keil, D., Mehlman, T., Proper, S., Shi, X., Harris, G.K., 2006. Essiac Tea: Scavenging of reactive oxygen species and effects on DNA damage. Journal of Ethnopharmacology. 103: 288-296 https://doi.org/10.1016/j.jep.2005.09.013
  13. Livingstone, D.R., 2001. Contaminant-stimulated reactive oxygen species production and oxidative damage in aquatic organisms, Mar. Pollut. Bull. 42: 656-666 https://doi.org/10.1016/S0025-326X(01)00060-1
  14. Li, Y., Trush, M.A., 1993. Oxidation of hydroquinone by copper: chemical mechanism and biological effects. Arch. Biochem. Biophys. 300: 346-355 https://doi.org/10.1006/abbi.1993.1047
  15. Lloyd, R.V., Hanna, P.M.,Mason, R.P., 1997. The origin of the hydroxyl radical oxygen in the Fenton reaction. Free Radic. Biol. and Med. 22: 885-888
  16. Powell, C.L., Swenberg, J.A., Rusyn, I., 2005. Expression of base excision DNA repair genes as a biomarker of oxidative DNA damage. Cancer Letters. 229: 1-11 https://doi.org/10.1016/j.canlet.2004.12.002
  17. Rosenkranz, A.R., Schmaldienst, S., Stuhlmeier, K.M., Chen, W., Knapp,W., Zlabinger, G.A., 1992.Microplate assay for the detection of oxidative products using 2',7'-dichlorofluorescindiacetate. J. Immunol. Methods. 156: 39-45 https://doi.org/10.1016/0022-1759(92)90008-H
  18. Schwarz, S.M., Peres, G., Kunz,W., Furstenberger, G., Kittstein, W.,Marks, F., 1984. On the role of superoxide anion radicals in skin tumour promotion. Carcinogenesis. 5: 1663-1670 https://doi.org/10.1093/carcin/5.12.1663
  19. Smirnoff, N., Cumbes, Q.J., 1989. Hydroxyl radical scavenging activity of compatible solutes. Phytochemistry. 28: 1057-1060 https://doi.org/10.1016/0031-9422(89)80182-7
  20. Stohs, S.J., Bagchi, D., 1995. Oxidative mechanism in the toxicity of metal ions. Free Radic. Biol. and Med. 18: 321-336 https://doi.org/10.1016/0891-5849(94)00159-H
  21. Wang, H., Gao, X.D., Zhou, G.C., Cai, L., YaO,W.B., 2008. In vitro and in vivo antioxidant activity of aqueous extract from Choerospondias axillaris furit. Food Chemistry. 106: 888-895 https://doi.org/10.1016/j.foodchem.2007.05.068