Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Identification and characterization of the phytocystatin family from Brassica rapa

  • Hong, Joon-Ki (Brassica Genomics Team, National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Hwang, Jung-Eun (Environmental Biotechnology National Core Research Centre and PMBBRC, Division of Applied Life Science(BK21 Program), Graduate School of Gyeongsang National University) ;
  • Park, Tae-Ho (Brassica Genomics Team, National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Zang, Yun-Xiang (Brassica Genomics Team, National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Lee, Sang-Choon (Brassica Genomics Team, National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Kwon, Soo-Jin (Brassica Genomics Team, National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Mun, Jeong-Hwan (Brassica Genomics Team, National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Kim, Hyun-Uk (Brassica Genomics Team, National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Kim, Jin-A (Brassica Genomics Team, National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Jin, Mi-Na (Brassica Genomics Team, National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Kim, Jung-Sun (Brassica Genomics Team, National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Lee, Soo-In (Brassica Genomics Team, National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Lim, Myung-Ho (Brassica Genomics Team, National Institute of Agricultural Biotechnology, Rural Development Administration)
  • Published : 2008.12.31
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Abstract

Phytocystatins, which are inhibitors of plant cysteine peptidases, are involved in the regulation of protein turnover and in the defense against insect pests and pathogens. Extensive searches in the Brassica rapa genome allowed the prediction of at least eight different phytocystatin genes on seven chromosomes in the B. rapa genome. Structure comparisons based on alignments of the all BrCYS ($\underline{B}$. $\underline{r}apa$ $phyto{\underline{cys}}tatin$) proteins using the CLUSTALW program revealed conservation of the three consensus motifs known to interact with the active site of cysteine peptidases. According to the phylogenetic analysis based on the deduced amino acid sequences, the eight BrCYS proteins were divided into several clusters related to the orthologous phytocystatin. The predicted three-dimensional structure models of the eight BrCYS proteins demonstrate that all of these proteins are similar to the reported crystal structure of oryzacystatin-I (OC-I). Digital northern and RT-PCR analyses indicated that the eight BrCYS genes exhibit different expression patterns in B. rapa tissues and respond differently to abiotic stimuli. The differences in gene structure and expression between the eight BrCYS genes suggest that these proteins may play diverse physiological roles in B. rapa and may interact with cysteine peptidases through different mechanisms.

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References

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