Tissue Engineering and Regenerative Medicine

Korean Tissue Engineering and Regenerative Medicine Society (한국조직공학·재생의학회)
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Effects of Low Intensity Ultrasound Stimulation on the Proliferation of Alveolar Bone Marrow Stem Cell

치조골 줄기세포 증식에 저강도 초음파 자극의 효과

  • Lee, Eung-Tae (Dept. of Biosystems & Biomaterial Sci Eng, Seoul Nat'l Univ.) ;
  • Lim, Ki-Taek (Dept. of Biosystems & Biomaterial Sci Eng, Seoul Nat'l Univ.) ;
  • Kim, Jang-Ho (Dept. of Biosystems & Biomaterial Sci Eng, Seoul Nat'l Univ.) ;
  • Im, Ae-Li (Dept. of Biosystems & Biomaterial Sci Eng, Seoul Nat'l Univ.) ;
  • Son, Hyun-Mok (Dept. of Biosystems & Biomaterial Sci Eng, Seoul Nat'l Univ.) ;
  • Cho, Chong-Su (School of Agricultural Biotech, Seoul Nat'l Univ.) ;
  • Choung, Pill-Hoon (Department of Oral and Maxillofacial Surgery, Tooth Bioengineering National Reserach Lab., Post-BK21, Intellectual Biointerface Engineering Center, Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Chung, Jong-Hoon (Dept. of Biosystems & Biomaterial Sci Eng, Seoul Nat'l Univ.)
  • 이응태 (서울대학교 농업생명과학대학 바이오시스템소재학부) ;
  • 임기택 (서울대학교 농업생명과학대학 바이오시스템소재학부) ;
  • 김장호 (서울대학교 농업생명과학대학 바이오시스템소재학부) ;
  • 임애리 (서울대학교 농업생명과학대학 바이오시스템소재학부) ;
  • 손현목 (서울대학교 농업생명과학대학 바이오시스템소재학부) ;
  • 조종수 (서울대학교 농업생명과학연구원) ;
  • 정필훈 (서울대학교 치과대학 구강악안면외과, 국가지정 치아생체공학연구실, 지능형생체계면공학연구센터, Post BK21, 서울대학교 치학연구소) ;
  • 정종훈 (서울대학교 농업생명과학대학 바이오시스템소재학부)
  • Published : 2008.12.01

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Abstract

Low-intensity ultrasound stimulation produces significant multi-functional effects that are directly relevant to bone formation. It was previously found that low-intensity ultrasound stimulation enhanced bone regeneration although the exact cellular mechanism is not clear. The aim of the present study is to investigate the effect of low-intensity ultrasound stimulation on proliferation of alveolar bone marrow stem cells. Before low-intensity ultrasound stimulation, alveolar bone marrow stem cells were cultured for 24h to facilitate their attachment. The cells were cultured in medium with or without low-intensity ultrasound stimulation. The ultrasound frequency was 1 MHz. Cell cultures stimulated with ultrasound were conducted by three treatment groups - group 1: intensity(100, 200, 300, 400 and 500 mW/$cm^{2}$), group 2: duty cycle(5, 10, 30 and 50%) and group 3: duration time(1, 3, 5, 10, 20 and 30 min). The effect of low-intensity ultrasound stimulation were evaluated by cell number and morphological changes. The proliferation rates of alveolar bone marrow stem cells for the particular stimulated groups were larger than those of control groups. After low-intensity ultrasound stimulation(intensity: 100 mW/$cm^{2}$, duty cycle: 30% and duration time: 10 min), the alveolar bone marrow stem cell counts were significantly increased(p < 0.05). This study suggested that the cell growth could be enhanced by appropriate low-intensity ultrasound stimulation.

Keywords