Korean Journal of Chemical Engineering

The Korean Institute of Chemical Engineers (한국화학공학회)
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Nitrifying bacterial communities and its activities in aerobic biofilm reactors under different temperature conditions

  • Park, Jeung-Jin (School of Civil and Environmental Engineering, Pusan National University) ;
  • Byun, Im-Gyu (School of Civil and Environmental Engineering, Pusan National University) ;
  • Park, So-Ra (School of Civil and Environmental Engineering, Pusan National University) ;
  • Park, Tae-Joo (School of Civil and Environmental Engineering, Pusan National University)
  • Published : 20081100
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Abstract

Tests were performed to investigate nitrifying bacterial communities and activities in aerobic biofilm reactors with different temperature conditions, denaturing gradient gel electrophoresis (DGGE) based on polymerase chain reaction targeting 16S rRNA and amoA gene, fluorescence in situ hybridization (FISH) and dehydrogenase activity (DHA). T1, T2 and T3 reactors operated at different temperatures (5, 10 and 30 ℃, respectively) were set up in the thermostat and acclimated. Nitrification was considerably limited in T1 and T2 reactors. DGGE revealed specific genera of ammonium-oxidizing bacteria (AOB) and some Nitrosomonas genera endured at the low temperatures. FISH revealed a decreased distribution ratio between AOB and nitrate-oxidizing bacteria at 5 ℃, and showed that the decrease of AOB also affected the nitrification failure in the aerobic biofilm reactor. The mean attached biomass of the T1, T2 and T3 reactors was 69.6, 80.6 and 112.9 mg/L, respectively, and the 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium chloride dehydrogenase activity of the respective reactors was 73.6, 87.4 and 134.2 $mgO_2^*$/g VSS/day. The results demonstrate that a low temperature condition in an aerobic biofilm reactor decreases the attached biomass, distribution ratio and activity of nitrifying bacteria, and produces a change in the composition of the AOB species, which results in the failure of nitrification.

Keywords

References

  1. Water Environment Federation, Wastewater treatment plant design, Alexandria, VA, 6-1-6-3 (2003)
  2. G. Tchbanoglous, F. L. Burton and H.D. Stensel, Wastewater engineering: treatment and reuse, 4th ed., McGraw-Hill, NY, 661-665 (2003)
  3. T. J. Park, K. H. Lee, D. S. Kim and C.W. Kim, Wat. Sci. Tech., 34 (10), 9 (1996)
  4. S. Zhu and S. Chen, Aquacult. Eng., 26, 221 (2002) https://doi.org/10.1016/S0144-8609(02)00022-5
  5. T. J. Park, K. H. Lee and J. H. Lee, Korean J. Chem. Eng., 15, 9 (1998) https://doi.org/10.1007/BF02705299
  6. R. Nogueira, V. Lazarova, J. Manem and L. F. Melo, Wat. Sci. Tech., 37(4), 189 (1998)
  7. P. Antoniou, J. Hamilton, B. Koopman, R. Jain, B. Holloway, G. Lyberatos and S. A. Svoronos, Wat. Res., 24(1), 97 (1990) https://doi.org/10.1016/0043-1354(90)90070-M
  8. Th. Willke and K.D. Vorlop, Progress in Biotech., 11, 718 (1996) https://doi.org/10.1016/S0921-0423(96)80097-9
  9. F. Fdz-Polanco, E. Méndez and S. Villaverde, Wat. Sci. Tech., 32(8), 227 (1995)
  10. C.N. Sawyer, P. L. McCarty and G. F. Parkin, Chemistry of environmental engineering, 4th ed., McGraw-Hill, NY, 658-659 (1994)
  11. H. Odegaard and B. Rusten, Norwegian experiences with nitrogen removal in a moving bed biofilm reactor, EWPCA-ISWA Symposium, Munchen(Germany), 11-14 May, 205-221 (1993)
  12. V. Lazarova, V. Pierzo, D. Fontviell and J. Manem, Wat. Sci. Tech., 29, 345 (1994) https://doi.org/10.2166/wst.1994.0361
  13. S.H. Hur, J. J. Park, Y. J. Kim, J. C. Yu, I.G. Byun, T.H. Lee and T. J. Park, Korean J. Chem. Eng., 24, 93 (2007) https://doi.org/10.1007/s11814-007-5015-2
  14. T. Imat, T. Kusuda and H. Furumat, Kinetic study and mathematical modeling of biofilm in an anaerobic fluidized bed, In Proc. 2nd Int. Specialized Conf. on Biofilm Reactors, 463-470, Paris, France (1993)
  15. N.R. Lazarova, J. Manem and L. Melo, Wat. Sci. Tech., 37(4), 189 (1998)
  16. Y. Liu and B. Capdeville, Wat. Res., 30(7), 1645 (1996) https://doi.org/10.1016/0043-1354(96)00069-3
  17. M. Wagner, G. Rath, R. Amann, H. P. Koops and K.H. Schleifer, Syst. Appl. Microbiol., 18, 251 (1995) https://doi.org/10.1016/S0723-2020(11)80396-6
  18. N. R. Pace, Science, 276, 734 (1997) https://doi.org/10.1126/science.276.5313.734
  19. V. Torsvik, L. Ovreas and T. F. Thingstad, Science, 296, 1064 (2002) https://doi.org/10.1126/science.1071698
  20. I. Cases and V. de Lorenzo, Environ. Microbiol., 4, 623 (2002) https://doi.org/10.1046/j.1462-2920.2002.00346.x
  21. G. Muyzer, S. Hottentrager, A. Teske and C. Wawer, Denaturing Gradient Gel Electrophoresis of PCR-Amplified 16S rDNA - A New Molecular Approach to Analyze the Genetic Diversity of Mixed Microbial Communities, Molecular Microbial Ecology Manual 3.4.4. (1996)
  22. G. Muyzer, Curr. Opin. Microbiol., 2, 317 (1999) https://doi.org/10.1016/S1369-5274(99)80055-1
  23. J.H. Rotthauwe, K. P. Witzel and W. Liesack, Appl. Environ. Microbiol., 63, 4704 (1997)
  24. G.A. Kowalchuk, P. L. E. Bodelier, G.H. J. Heilig, J.R. Stephen and H. J. Laanbroek, FEMS Microbiol. Ecol., 27, 339 (1998) https://doi.org/10.1111/j.1574-6941.1998.tb00550.x
  25. C. D. Sinigalliano, D. N. Kuhn and R. D. Jones, Appl. Environ. Microbiol., 61, 2702 (1995)
  26. M. Wagner, M. Schmid, S. Juretschko, K.H. Trebesius, A. Bubert, W. Goebel and K.H. Schleifer, FEMS Microbiol. Lett., 160, 159 (1998) https://doi.org/10.1111/j.1574-6968.1998.tb12906.x
  27. C. Wawer, M.M. Jetten and G. Muyer, Appl. Environ. Microbiol., 63, 4360 (1997)
  28. U. Purkhold, A. Pommerening-Rosor, S. Juretschko, M. C. Schmid, H. P. Koops and M. Wagner, Appl. Environ. Microbiol., 66, 5368 (2000) https://doi.org/10.1128/AEM.66.12.5368-5382.2000
  29. D. J. Kim, D. I. Lee and J. Keller, Bioresource Technol., 97, 459 (2006) https://doi.org/10.1016/j.biortech.2005.03.032
  30. A. Schramm, D. De Beer, M. Wagner and R. Amann, Appl. Environ. Microbiol., 64(9), 3480 (1998)
  31. A. Jang, P. L. Bishop, S. Okabe, S.G. Lee and I. S. Kim, Wat. Sci. Tech., 47(1), 49 (2002)
  32. H. Yang, Z. Jiang, S. Shi and W. Z. Tang, Ecotoxicology and Environmental Safety, 53, 416 (2002) https://doi.org/10.1016/S0147-6513(02)00002-7
  33. G. Bitton and B. Koopman, Appl. Environ. Microbiol., 43, 964 (1982)
  34. B. Koopman, G. Bitton, C. Logue, J. M. Bossart and J. M. Lopez, Validity of tetrazolium reduction assays for assessing toxic inhibition of filamentous bacteria in activated sludge, In Toxicity Screening Procedures using Bacterial Systems, 147-162, NY, USA (1984)
  35. J. Awong, G. Bitton and B. Koopman, Wat. Res., 19, 917 (1985) https://doi.org/10.1016/0043-1354(85)90151-4
  36. C.W. Kim, B. Koopman and G. Bitton, Wat. Res., 28(5), 1117 (1994) https://doi.org/10.1016/0043-1354(94)90198-8
  37. Water Environment Federation, Biological and chemical systems for nutrient removal, Alexandria, VA, 69-73 (1998)
  38. M.H. Nicolaisen and N. B. Ramsing, J. of Microbiol. Metho., 50, 189 (2002) https://doi.org/10.1016/S0167-7012(02)00026-X
  39. Y. Suzuki, T. Yoda, A. Ruhul and W. Sugiura, J. Biol. Chem., 276 (12), 9059 (2001) https://doi.org/10.1074/jbc.M008083200
  40. H. Daims, A. Bruhl, R. Amann, K.-H. Schleifer and M. Wagner, Systematic and Applied Microbiology, 22(3), 434 (1999) https://doi.org/10.1016/S0723-2020(99)80053-8
  41. B.K. Mobarry, M. Wagner, V. Urbain, B. E. Rittmann and D.A. Stahl, Applied and Environmental Microbiology, 62(6), 2156 (1996)
  42. H. Daims, P.H. Nielsen, J. L. Nielsen, S. Juretschko and M. Wagner, Water science and technology, 41(4-5), 85 (2000)
  43. M. Wagner, G. Rath, H.-P. Koops, J. Flood and R. Amann, Water Science and Technology, 34(1), 237 (1996)
  44. S.M. Lee, J. Y. Jung and Y. C. Chung, Biotech. Letters, 22, 991 (2000) https://doi.org/10.1023/A:1005637203643
  45. J.H. Lim, Analysis of nitrification in biofilm using biochemical methods, PhD thesis, Department of environmental engineering, Pusan National University, Korea (2003)
  46. M.H. Nicolaisen and N. B. Ramsing, J. Microbiol. Methods, 50, 189 (2002) https://doi.org/10.1016/S0167-7012(02)00026-X
  47. M. Wagner and A. Loy, Biotechnology, 13, 218 (2002)
  48. C. L. Laia and G. G. Jesus, J. Microbiol. Metho., 57(1), 69 (2004) https://doi.org/10.1016/j.mimet.2003.11.019