Korean Chemical Engineering Research

The Korean Institute of Chemical Engineers (한국화학공학회)
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Biodegradation of JP-8 by Rhodococcus fascians Isolated from Petroleum Contaminated Soil

유류 오염 토양에서 분리된 Rhodococcus fascians를 이용한 JP-8의 분해 특성

  • Nam, Bo Hyun (Department of Biological Engineering, Inha University) ;
  • Park, Bong Je (Department of Biological Engineering, Inha University) ;
  • Yun, Hyun Shik (Department of Biological Engineering, Inha University)
  • Received : 2008.05.08
  • Accepted : 2008.06.05
  • Published : 2008.08.31
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Abstract

Microorganisms isolated from petroleum contaminated site were tested for their ability to grow on JP-8 by culturing them on the culture medium that contains JP-8 as a carbon source. The microorganism which grew on JP-8 containing minimal salt medium was separated and identified as Rhodococcus fascians. Changes in JP-8 biodegradation of R. fascians that was isolated from petroleum contaminated site was investigated with various inoculums sizes, JP-8 concentrations, medium pHs, and culture temperatures. The amount of JP-8 was analyzed by TPH using Gas Chromatography.

유류 오염 토양으로부터 항공유 JP-8을 분해하는 능력이 우수한 균주를 분리하여 16S rDNA 염기서열 분석한 결과 Rhodococcus fascians로 확인되었다. 분리된 R. fascians를 MSM 배지에 JP-8을 탄소원으로 배양하여 그 성장특성과 JP-8 분해 특성을 고찰하였다. 분리된 R. fascians는 2% JP-8을 포함하는 배지에서 5일 이내에 JP-8 대부분을 분해하였다. 16% JP-8을 포함하는 배지에서는 10일 동안 배양액 내 JP-8의 농도를 초기 농도의 14%까지 낮출 수 있었다. $17^{\circ}C$ 부터 $32^{\circ}C$ 까지 JP-8의 분해에 미치는 영향을 살펴본 결과 5일 후에는 모든 시료에서 JP-8의 대부분이 제거되었으며 온도가 낮을수록 R. fascians에 의하여 분해되는 JP-8의 양이 많은 것으로 나타났다. 배지의 초기 pH는 pH5에서 초기 분해속도가 비교적 낮았으나 pH8 까지 모두 5일만에 JP-8의 대부분이 제거되었다.

Keywords

Acknowledgement

Supported by : 인하대학교

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