Korean Chemical Engineering Research

The Korean Institute of Chemical Engineers (한국화학공학회)
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Preparation of Silica/collagen Microsphere Composit Doped with Silver Nanoparticles

은 나노입자를 담지한 collagen/silica microsphere 복합체의 제조

  • Jung, Hyo Jung (Department of Chemical Engineering, Inha University) ;
  • Kim, Yeon Bum (Department of Chemical Engineering, Inha University) ;
  • Chang, Yoon Ho (Department of Chemical Engineering, Inha University)
  • 정효정 (인하대학교 공과대학 화학공학과) ;
  • 김연범 (인하대학교 공과대학 화학공학과) ;
  • 장윤호 (인하대학교 공과대학 화학공학과)
  • Received : 2008.04.10
  • Accepted : 2008.05.08
  • Published : 2008.08.31
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Abstract

Silica microsphere is a world leading innovative material used in adsorbent packing materials in HPLC technology. The application of microsphere lies in the ability to the surface modification of silica with the special materials such as polymers, metals and bio-active materials. Collagen is a major structural protein of connective tissues and has a good biocompatibility. In this study, we prepared the purified silica porous microsphere, having micro diameters in the range of a pore volume at least 50% by the aggregation procedure of colloidal silica with the polymerization method (PICA). The microspheres were modified by collagen hydrogel to improve the biocompatible properties for biomedical product. The silica/collagen microsphere composite doped with silver nanoparticles was prepared and investigated the capabilities of biomaterial application through the evaluation of the structure characteristics of the microsphere composit.

실리카 microsphere는 HPLC를 위한 흡착 충진제와 같은 용도로 사용하기에 적합한 혁신적인 소재로 널리 알려져 있다. microsphere을 기능성고분자나 금속, 생리활성 물질과 같은 특정한 성질을 지닌 물질로 표면 개질시키므로 다양한 용도로 활용할 수 있다. 콜라겐은 생체조직을 구성하는 기본 단백질로 생체적합성이 뛰어난 물질로 주목받고 있는 기능성 소재이다. 본 연구에서는 50% 이상의 세공부피를 지닌 다공성 silica microsphere를 고분자 응집법인 PICA 법을 이용하여 colloidal silica로부터 제조하고 콜라겐 hydrogel을 사용하여 표면 개질시키므로 생체적합성을 증진시키는 방법을 연구하였다. 실리카/콜라겐 microsphere 에 은 나노입자를 담지시킨 microsphere 복합체를 만들고 특성을 조사하므로 생체소재로의 활용 가능성을 조사하였다.

Keywords

References

  1. Yang, L. J. and Li, Y. B., "Quantum Dots as Fluorescent Labels for Quantitative Detection of Salmonella Typhimurium in Chicken Carcass Wash Water," J. Food Prot., 68, 1241-1245(2005) https://doi.org/10.4315/0362-028X-68.6.1241
  2. Miller, D. K., et al., "Development of a High-capacity Homogeneous Fluorescent Assay for the Measurement of Leukotriene B-4," Anal. Biochem., 349, 129-135(2006) https://doi.org/10.1016/j.ab.2005.10.043
  3. Murakami, Y., Endo, T., Yamamura, S., Nagatani, N., Takamura, Y. and Tamiya, E., "On-chip Micro-flow Polystyrene Bead-based Immunoassay for Quantitative Detection of Tacrolimus (FK506)," Anal. Biochem., 334, 11-16(2004)
  4. Yang, L., Tran, D. K. and Wang, X., "BADGE, Beads Array for the Detection of Gene Expression, a High-throughput Diagnostic Bioassay," Genome Res., 11, 1888-1898(2001)
  5. Marquette, C. A. and Blum, L. J., "Immobilisation of DNA on Chips Ii (Berlin: Springer)," 113-129(2005)
  6. Huang, Z. L., Zhao, Y. D. and Luo, Q. M., "Quantum-dot-tagged Microbeads and Their Use as Fluorescent Biological Probes. Curr," Anal. Chem., 2, 9-66(2006)
  7. Kim, T. H., Kim, J. Y., Kim, M. J., "Prepration of Silica Nanoparticles Using Water in Oil Microemulsion," HWAHAK KONGHAK, 41(2), 174-185(2003)
  8. van, Sark. W., Frederix, P., Bol, A. A., Gerritsen, H. C. and Meijerink, A., "Blueing, Bleaching, and Blinking of Single CdSe/ZnS Quantum dots," Chem. Phys. Chem., 3, 871-879(2002) https://doi.org/10.1002/1439-7641(20021018)3:10<871::AID-CPHC871>3.0.CO;2-T
  9. Nirmal, M., Dabbousi, B. O., Bawendi, M. G., Macklin, J. J., Trautman, J. K., Harris, T. D. and Brus, L. E., "Fluorescence Intermittency in Single Cadmium Selenide Nanocrystals," Nature, 383, 802-804(1996) https://doi.org/10.1038/383802a0
  10. Kirchner, C., Liedl, T., Kudera, S., Pellegrino, T., Javier, A. M., Gaub, H. E., Stolzle, S., Fertig, N. and Parak, W. J., "Cytotoxicity of Colloidal CdSe and CdSe/ZnS Nanoparticles," Nano Lett., 5, 331-338(2005) https://doi.org/10.1021/nl047996m
  11. Hoshino, A., Fujioka, K., Oku, T., Suga, M., Sasaki, Y. F., Ohta, T., Yasuhara, M., Suzuki, K. and Yamamoto, K., "Physicochemical Properties and Cellular Toxicity of Nanocrystal Quantum Dots Depend on Their Surface Modification," Nano Lett., 4, 2163-2169(2004) https://doi.org/10.1021/nl048715d
  12. Wang, F., Zhang, Y., Fan, X. P. and Wang, M. Q., "One-pot synthesis of Chitosan/LaF3:Eu3+nanocrystals for Bio-applications," Nanotechnology, 17, 1527-1532(2006) https://doi.org/10.1088/0957-4484/17/5/060
  13. Wang, F., Zhang, Y., Fan, X. P. and Wang, M. Q., "Facile Synthesis of Water-soluble LaF3:Ln(3+) Nanocrystals," J. Mater. Chem., 16, 1031-1034(2006) https://doi.org/10.1039/b518262j
  14. Lee, S. W., Lee, C. I., Lee, Y., Chang, Y. H. and Hahm, Y. M., "Study on the Size Control of Spherical Silica Particles Prepared by Colloid - Aggregation Method," Appl. Chem., 5(2), 256-259(2001)
  15. Thibaud, Coradin., Marie-Madeleine Giraud-Guille., Christophe, Helary., Jacques, Livage. and Clment Sanchez., "Association of Silica with Biopolymers for the Design of New Biocomposites," Mat. Res. Soc. Symp. Proc., 726(2002)
  16. Fagundes, L. B., Sousa, T. G. F., Sousa, A., Silva, V.V., Sousa, E. M. B., "SBA-15-collagen Hybrid Material for Drug Delivery Applications," J. Non-Crystal. Solids, 352, 3496-3501(2006) https://doi.org/10.1016/j.jnoncrysol.2006.03.111
  17. Izquierdo-Barba, Africa Martinez., Antonio L. Doadrio, Joaquin Perez-Pariente, Maria Vallet-Reg., "Release Evaluation of Drugs from Ordered Three-dimensional Silica Structures," Eur. J. Pharm. Sci., 26, 365-373(2005) https://doi.org/10.1016/j.ejps.2005.06.009
  18. Ryu, J. H., Chang, D. S., Choi, B. G., Yoon, J. W., Lim, C. S., Shim, K. B., "Fabrication of Ag Nanoparticles-coated Macroporous SiO2 Structure by Using Polystyrene Spheres, " Mat. Chem. Phys., 101, 486-491(2007)
  19. Radhesh, Kumar., Steve, Howdle., Helmut, Munstedt., "Polyamide/ silver Antimicrobials: Effect of Filler Types on the Silver Ion Release," J. Biomed. Mater. Res. Part B: Appl. Biomater., 75B, 311-319(2005) https://doi.org/10.1002/jbm.b.30306