Archives of Plastic Surgery

Korean Society of Plastic and Reconstructive Surgeons (대한성형외과학회)
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Gastroepiploic Artery Preservation with Glycerol as a Training Model for Microvascular Anastomosis

미세혈관문합술의 연습 모델로서 글리세롤 처리된 위대망동맥의 활용

  • Park, Chan Young (Department of Plastic and Reconstructive Surgery, Research Institute of Plastic and Reconstructive Surgery, Seoul National University College of Medicine) ;
  • Chang, Hak (Department of Plastic and Reconstructive Surgery, Research Institute of Plastic and Reconstructive Surgery, Seoul National University College of Medicine) ;
  • Chung, Jin Haeng (Department of Pathology, Research Institute of Plastic and Reconstructive Surgery, Seoul National University College of Medicine) ;
  • Heo, Eun Ju (Department of Plastic and Reconstructive Surgery, Research Institute of Plastic and Reconstructive Surgery, Seoul National University College of Medicine) ;
  • Minn, Kyung Won (Department of Plastic and Reconstructive Surgery, Research Institute of Plastic and Reconstructive Surgery, Seoul National University College of Medicine) ;
  • Yoo, Moon Won (Department of Surgery, Research Institute of Plastic and Reconstructive Surgery, Seoul National University College of Medicine) ;
  • Yang, Han Kwang (Department of Surgery, Research Institute of Plastic and Reconstructive Surgery, Seoul National University College of Medicine)
  • 박찬영 (서울대학교 의과대학 성형외과학교실, 성형재건연구소) ;
  • 장학 (서울대학교 의과대학 성형외과학교실, 성형재건연구소) ;
  • 정진행 (서울대학교 의과대학 병리학교실, 성형재건연구소) ;
  • 허은주 (서울대학교 의과대학 성형외과학교실, 성형재건연구소) ;
  • 민경원 (서울대학교 의과대학 성형외과학교실, 성형재건연구소) ;
  • 유문원 (서울대학교 의과대학 외과학교실, 성형재건연구소) ;
  • 양한광 (서울대학교 의과대학 외과학교실, 성형재건연구소)
  • Received : 2008.05.13
  • Accepted : 2008.06.20
  • Published : 2008.10.15
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Abstract

Purpose: We investigate the possibility of vessel preservation with glycerol and evaluate the potential of preserved gastroepiploic artery as a tool for the microsurgical practice. Methods: In 5 early gastric cancer patients, IRB(No. C-0603-040-170), we gained gastroepiploic artery specimens(5 segments, about 10 cm) after gastrectomy. Each segment was rinsed in a normal saline and subsequently placed in a bottle, containing 50 mL anhydrous glycerol (87%). The bottles were refrigerated at $4^{\circ}C$, the longest preservation time being 5 months. Results: At first glance, no fragmentation was detected and the surface of vessel seemed mild sclerotic. In histological examination, vascular structures remained intact though preservation with glycerol led to a mild atrophy of the smooth muscle in the tunica media. Especially, we found out the elastic fibers and endothelial lining of the intima were preserved until 5 months. Adequate status for using microsurgical practice was also maintained and leakage test was performed successfully with gentian violet ink. Conclusion: Based on the results obtained in this study, the gastroepiploic artery preservation with glycerol as a training model for microvascular anastomosis is a technically very simple procedure and useful for the novice microsurgeon.

Keywords

Acknowledgement

Supported by : 한국과학재단

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