Journal of Veterinary Science

The Korean Society of Veterinary Science (대한수의학회)
권호 표지

The radioprotective effects of the hexane and ethyl acetate extracts of Callophyllis japonica in mice that undergo whole body irradiation

Kim, Jeong-Tae;Moon, Chang-Jong;Kim, Hee-Chul;Jeong, Jin-Woo;Lee, Ju-Yeon;Kim, Ji-Hoon;Hyun, Jin-Won;Park, Jae-Woo;Moon, Mi-Yeon;Lee, Nam-Ho;Kim, Sung-Ho;Jee, Young-Heun;Shin, Tae-Kyun

  • Published : 20080900
⟨ Previous Next ⟩

Abstract

The radioprotective activity of extracts from the red seaweed Callophyllis (C.) japonica was investigated in mice that underwent whole-body exposure to gamma radiation. A methanol extract of C. japonica and its fractions [hexane, ethyl acetate (EtOAc), butanol and the remaining H2O] were used. Each fraction (100 mg/kg body weight) was administered intraperitoneally (i.p.) 2 times into the BALB/c mice, once at 1 and once at 24 h before exposure to 9 Gray (Gy) of gamma radiation. Pre-irradiation administration of the hexane and EtOAc fractions saved the mice, with their survival rates being greater than 80% at 30 days post-irradiation; the mice that were pretreated with the other fractions showed survival rates lower than 20% over the same time period. To examine the effect of each C. japonica fraction on the survival of intestinal and bone marrow stem cells, the number of intestinal crypts and bone marrow cells in the gamma-irradiated mice were examined. Pre-treatment of mice (i.p., 100 mg/kg body weight at 1 and 24 h before irradiation) with the hexane or EtOAc fraction prior to 6-Gy irradiation significantly protected the number of jejunal crypts and bone marrow cells at 9 days after irradiation. These findings suggest that certain extracts from C. japonica, when they are administered prior to irradiation, play an important role in the survival of irradiated mice, and this is possibly due to the extracts protecting the hematopoietic cells and intestinal stem cells against gamma irradiation.

Keywords

References

  1. Ahn M, Kim H, Kim JT, Lee J, Hyun JW, Park JW, Shin T. Gamma-ray irradiation stimulates the expression of caveolin-1 and GFAP in rat spinal cord: a study of immunoblot and immunohistochemistry. J Vet Sci 2006, 7, 309-314 https://doi.org/10.4142/jvs.2006.7.4.309
  2. Hosseinimehr SJ. Trends in the development of radioprotective agents. Drug Discov Today 2007, 12, 794-805 https://doi.org/10.1016/j.drudis.2007.07.017
  3. Kang KA, Bu HD, Park DS, Go GM, Jee Y, Shin T, Hyun JW. Antioxidant activity of ethanol extract of Callophyllis japonica. Phytother Res 2005, 19, 506-510 https://doi.org/10.1002/ptr.1692
  4. Karbownik M, Reiter RJ, Qi W, Garcia JJ, Tan DX, Manchester LC, Vijayalaxmi. Protective effects of melatonin against oxidation of guanine bases in DNA and decreased microsomal membrane fluidity in rat liver induced by whole body ionizing radiation. Mol Cell Biochem 2000, 211, 137-144 https://doi.org/10.1023/A:1007148530845
  5. Kim H, Moon C, Kim J, Ahn M, Hyun JW, Park JW, Kim SH, Kim S, Shin T. Increased phosphorylation of caveolin-1 in the spinal cord of irradiated rats. J Vet Sci 2007, 8, 323-327 https://doi.org/10.4142/jvs.2007.8.4.323
  6. Kim JT, Kim H, Shin T. Effect of ethanol extract of Callophyllis japonica in rats with irradiation. J Appl Radiol Sci Res Inst Cheju Natl Univ 2006, 20, 31-35
  7. Miura Y. Oxidative stress, radiation-adaptive responses, and aging. J Radiat Res 2004, 45, 357-372 https://doi.org/10.1269/jrr.45.357
  8. Moon C, Kim SH, Kim JC, Hyun JW, Lee NH, Park JW, Shin T. Protective effect of phlorotannin components phloroglucinol and eckol on radiation-induced intestinal injury in mice. Phytother Res 2008, 22, 238-242 https://doi.org/10.1002/ptr.2298
  9. Nair CK, Parida DK, Nomura T. Radioprotectors in radiotherapy. J Radiat Res 2001, 42, 21-37 https://doi.org/10.1269/jrr.42.21
  10. Park DS, Lee KH, Kim HC, Ahn MJ, Moon C, Ko MS, Lee KK, Go GM, Shin T. Effects of Callophyllis japonica powder on carbon tetrachloride-induced liver injury in rats. Orient Pharm Exp Med 2005, 5, 231-235 https://doi.org/10.3742/OPEM.2005.5.3.231
  11. Potten CS, Grant HK. The relationship between ionizing radiation-induced apoptosis and stem cells in the small and large intestine. Br J Cancer 1998, 78, 993-1003 https://doi.org/10.1038/bjc.1998.618
  12. Schuchter LM. Guidelines for the administration of amifostine. Semin Oncol 1996, 23, 40-43
  13. Weil MM, Stephens LC, Amos CI, Ruifrok AC, Mason KA. Strain difference in jejunal crypt cell susceptibility to radiation-induced apoptosis. Int J Radiat Biol 1996, 70, 579-585 https://doi.org/10.1080/095530096144789
  14. Withers HR, Elkind MM. Microcolony survival assay for cells of mouse intestinal mucosa exposed to radiation. Int J Radiat Biol Relat Stud Phys Chem Med 1970, 17, 261-267 https://doi.org/10.1080/09553007014550291
  15. Zhou Y, Mi MT. Genistein stimulates hematopoiesis and increases survival in irradiated mice. J Radiat Res 2005, 46, 425-433 https://doi.org/10.1269/jrr.46.425