Journal of Korean Society on Water Environment (한국물환경학회지)

Korean Society on Water Environment (한국물환경학회)
권호 표지

Microbial Community in the TPH-Contaminated Aquifer for Hot Air Sparging using Terminal-Restriction Fragment Length Polymorphism

유류오염대수층 고온공기분사공정시 제한효소다형성 미생물 군집

  • Lee, Junho (Department of Environmental Science and Engineering, Hankuk University of Foreign Studies) ;
  • Park, Kapsong (Department of Environmental Science and Engineering, Hankuk University of Foreign Studies)
  • 이준호 (한국외국어대학교 자연과학대학 환경학과) ;
  • 박갑성 (한국외국어대학교 자연과학대학 환경학과)
  • Received : 2007.04.10
  • Accepted : 2007.12.17
  • Published : 2008.01.30
Download PDF
⟨ Previous Next ⟩

Abstract

Hot air sparging is a groundwater remediation technique, in which organic contaminants volatilized into hot air from the saturated to vadose zone. In the laboratory diesel (10,000 mg TPH/kg) was spiked in contaminated saturated aquifer soil. The hot air ($34.9{\pm}2.7^{\circ}C$) was injected in intermittent (Q=1,500 mL/min, 10 minute injection and 10 minute idle) modes. We performed microcosm tests using the groundwater samples to assess TPH reductive remediation activity. For Terminal-Restriction Fragment Length Polymorphism (T-RFLP) analysis of eubacterial communities in sludge of wastewater treatment plants and soil of experiment site, the 16S rDNA was amplified by Polymerase Chain Reaction (PCR) from the sludge and the soil. The obtained 16S rDNA fragments were digested with Msp I and separated by electrophoresis gel. We found various sequence types for hot air sparging experiment with sludge soil samples that were closely related to Bacillus (149 bp, Firmicutes), Methlobacterium (149 bp, Euryarchaeotes), Pseudomonas (492 bp, ${\gamma}$-Proteobacteria), etc., in the clone library. In this study we find that TPH-water was reduced to 78.9% of the initial value in this experiment aquifer. The results of the present study suggests that T-RFLP method may be applied as a useful tool for the monitoring in the TPH contaminated soil fate of microorganisms in natural microbial community.

Keywords

Acknowledgement

Supported by : 한국외국어대학교

References

  1. 김재수(2006). 주요 오염물질로 오염된 지하수에서 미생물 의 무배양식 군집분석방법과 미생물상에 대한 조사방법 연구. 한국지하수토양환경학회지, 11(3), pp. 66-77
  2. 송정훈, 장순웅, 이시진(2002). 오염지하수 정화를 위한 공 기주입정화법. 2002년 산업기술종합연구소 논문집, 23, pp. 129-143
  3. 안난희, 장덕진(2002). 생물공학의 동향X I-A: 활성슬러지 내에 서식하는 세균군집의 RFLP 분석. 한국생물공학회, pp. 320-323
  4. 정성영(2001). 말단 제한 절편 다형성 분석법을 이용한 유 류 분해 미생물의 동태 추적. 석사학위논문, 한양대학교
  5. Autry, A. R. and Ellis, G. M. (1992). Bioremediation: an Effective Remedial Alternative for Petroleum Hydrocarbon Contaminated Soil. Environmental progress, 11, pp. 318-323 https://doi.org/10.1002/ep.670110423
  6. Braker, G., Zhou, J., Wu, L., Devol, A. H. and Tiedje, J. M. (2004). Nitrate reductase genes (nirK and nirS) as functional markers to investigate diversity of denitrifying bacteria in Pacific Northwest marine sediment communities. Appl. Environ. Microbiol., 66, pp. 2096-2104 https://doi.org/10.1128/AEM.66.5.2096-2104.2000
  7. Carter, M. R. (1993). Soil Sampling and Methods of Methods of Analysis, Lewis
  8. Cline, S. R. (1993). Efficiencies of Soil Washing/Flushing Solutions for the Remediation of Lead Contaminated Soil. Thesis, West Virginia University
  9. Cookson, J. T. (1995). Bioremediation Engineering Design and Application, McGraw-Hill, Inc
  10. Garland, J. L. (1997). Analysis and interpretation of community- level physiological profiles in microbial ecology. FEMS Microbiol. Ecol., 24, pp. 289-300 https://doi.org/10.1111/j.1574-6941.1997.tb00446.x
  11. Hinchee, R. E. (2000). Air Sparging for Site Remediation, Lewis Publishers
  12. Kleikemper, J., Schroth, M. H., Sigler, W. W., Schmucki, M., Bernasconi, S. M. and Zeyer, J. (2002). Activity and diversity of sulfate-reducing bacteria in a petroleum hydrocarbon- contaminated aquifer. Appl. Environ. Microbiol., 68, pp. 1516-1523 https://doi.org/10.1128/AEM.68.4.1516-1523.2002
  13. Klein, A. and Schnorr, M. (1984). Genome complexity of methanogenic bacteria. J. Bacteriol., 158(2), pp. 626-631
  14. Lovely, D. R. (1993). Dissimilatory metal reduction. Ann. Rev. Microbial., 47, pp. 263-290 https://doi.org/10.1146/annurev.mi.47.100193.001403
  15. Nealson, K. H. and Saffarini, D. (1994). Iron and manganese in anaerobic respiration: environmental significance, physiology, and regulation. Ann. Rev. Microbiol., 48, pp 311-343 https://doi.org/10.1146/annurev.mi.48.100194.001523
  16. Peterson, J. W., Deboer, M. D. and Lake, K. L. (2000). A Simulation of Toluene Cleanup by Air Sparging of Water Saturated Sands. Journal of Hazardous Materials, 72, pp. 167-178 https://doi.org/10.1016/S0304-3894(99)00139-9
  17. Philippot, L. (2005). Tracking nitrate reducers and denitrifiers in the environment. Biochem. Soc. Trans., 33(1), pp. 200-204 https://doi.org/10.1042/BST0330200
  18. Smidt, H. and de Vos, W. M. (2004). Anaerobic microbial dehalogenation. Annu. Rev. Microbial., 58, pp. 43-73 https://doi.org/10.1146/annurev.micro.58.030603.123600
  19. US EPA. (1992). A Technology Assessment of Soil Vapor Extraction and Air Sparging. EPA/600/R-92/173
  20. Warren, E. B. B., Godsy, E. and Smith, V. (2004). Inhibition of acetoclastic methanogenesis in crude oil and creosotecontaminated groundwater. Bioremediation J., 8, pp. 1-11 https://doi.org/10.1080/10889860490465840