Food Engineering Progress (산업식품공학)

Korean Society for Industrial Food Engineering (한국산업식품공학회)
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Detection and Identification of Shiga-like Toxin Producing Escherichia coli O157:H7 by Multiplex PCR

Multiplex PCR을 이용한 Shiga-like toxin 생성 Escherichia coli O157:H7의 검출 및 확인

Lim, Ji-Su;Yoon, Jang-Ho;Min, Bok-Kee;Hong, Kwang-Won
임지수;윤장호;민복기;홍광원

  • Published : 2008.03.10
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Abstract

A multiplex polymerase chain reaction method was developed for rapid detection and identification of Shiga-like toxin producing Escherichia coli O157:H7 in food. Specificity of 15 primer sets that target genes specific for E. coli O157:H7 were tested on five E. coli O157:H7 strains, three other serotypes of E. coli and ten non-E. coli strains using singleplex PCR. Among them, four primer sets for the E. coli O157:H7 genes rfbE, eaeA, stxI and stxII genes were selected for a multiplex PCR assay. All E. coli O157:H7 examined were positive for the rfbE and eaeA genes, however, the amplifications of Shiga-like toxin genes stxI and stxII were dependent on the strains. The detection limit of the multiplex PCR assay was 2.2x101CFU/reaction in artificially inoculated lettuce without enrichment. This multiplex PCR method could be used to check the presence of E. coli O157:H7 and Shiga-like toxin producing E. coli strains in food.

Keywords

References

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