Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
권호 표지

Nitrogen Source and Sucrose Concentration in the Medium Affect Indian ginseng (Withania somniefera) Cultures in Vitro

MS배지 내 질소원 및 당 농도가 Indian ginseng의 기내 배양에 미치는 영향

Lee, Sang-Ho;Hahn, Eun-Joo;Paek, Kee-Yoeup
이상호;한은주;백기엽

  • Published : 20080900
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Abstract

To establish micropropagation system of Indian ginseng (Withania somnifera) plantlets having anti-cancer efficacy, differential NH₄⁺:NO₃⁻ ratios and sucrose concentrations in MS medium were tested. Plantlet growth was the greatest at a 20:40 (mM) NH₄⁺:NO₃⁻ ratio, while significantly inhibited when NH₄⁺ was used as a sole nitrogen source. Among the sucrose concentrations, 3% sucrose resulted in the highest shoot and root growth; however, increasing of sucrose concentration up to 7% induced a severe growth retard due to an excessive increase of water potential in the medium. High levels of sucrose in the medium increased leaf stomatal density but decreased stomata size, considered as a compensation activity against stressful environment.

최근 항암효과를 나타내는 withaferine-A를 함유하고 있어 천연 의약품 개발을 위한 자원으로 생산되고 있는 Indian ginseng(W. somnifera)의 기내 대량 번식을 위해 MS배지 내질소원의 비율과 당 농도에 따른 식물체의 생장에 대한 실험을 실시하였다. 식물체의 생장은 배지의 NH₄⁺:NO₃⁻ 비율이 20:40(mM) 이었던 처리구에서 가장 좋았고 NH₄⁺ 단용 처리구에서 가장 크게 억제되었다. 배지 내 당 농도의 경우, 3% 처리구에서 지상부와 지하부 생장이 가장 양호하였고 5또는 7%로 높아질수록 배지 내 삼투압의 지나친 증가로 생장이 현저히 저하되었다. 배지 내 고농도의 당은 잎의 단위 면적 당 기공밀도는 증가시켰던 반면, 기공의 크기를 크게 감소시켜 스트레스에 의한 보상 작용임을 알 수 있었다.

Keywords

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