Korean Journal of Chemical Engineering

The Korean Institute of Chemical Engineers (한국화학공학회)
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Enhanced stability of Candida antarctica lipase B in ionic liquids

  • Ha, Sung-Ho (ERC for Advanced Bioseparation Technology, Inha University) ;
  • Lee, Sang-Hyun (ERC for Advanced Bioseparation Technology, Inha University) ;
  • Dang, Dung-Thanh (Department of Biological Engineering, Inha University) ;
  • Kwon, Min-Sik (Department of Biological Engineering, Inha University) ;
  • Chang, Woo-Jin (ERC for Advanced Bioseparation Technology, Inha University) ;
  • Yu, Yong-Jae (C-TRI) ;
  • Byun, Il-Suk (C-TRI) ;
  • Koo, Yoon-Mo (ERC for Advanced Bioseparation Technology, Inha University)
  • Published : 20080000
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Abstract

The activity and stability of lipase from Candida antarctica were investigated in the kinetic resolution of (R,S)-1-phenylethanol with vinyl acetate using ionic liquids (ILs) as reaction media. Among ILs tested, the highest activity of lipase was observed in [Edmim][$Tf_2N$]. In hydrophobic ILs such as [Edmim][$Tf_2N$], [Emim][$Tf_2N$] and [Pmim] [$PF_6$], lipase could retain its activity after 5 times reuse, while the activity of lipase in hydrophilic ILs and organic solvents was drastically decreased. The activities of lipase in [Edmim][$Tf_2N$], [Emim][$Tf_2N$] and [Pmim][$PF_6$] were also well maintained after 1 day incubation at 80 ℃. The lipase suspended in [Edmim][$Tf_2N$] could be successfully reused 6 times without loss of activity.

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