Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Study on Genetic Diversity of Six Duck Populations with Microsatellite DNA

  • Wu, Yan (Institute of Animal Science, Chinese Academy of Agricultural Science) ;
  • Liu, Xiao-Lin (College of Animal Science and Technology, Northwest A＆F University) ;
  • Hou, Shui-Sheng (Institute of Animal Science, Chinese Academy of Agricultural Science) ;
  • Huang, Wei (Institute of Animal Science, Chinese Academy of Agricultural Science)
  • Received : 2007.06.30
  • Accepted : 2007.11.26
  • Published : 2008.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, we investigated the genetic diversity and phylogenetic relationship of six duck populations by employing the genetic polymorphisms of 20 microsatellites. The parameters used in this study included number of alleles, average effective numbers of alleles (E) and average rates of heterozygosity of each population. The results showed that all the microsatellite loci were highly polymorphic except that the locus AJ515896 in Muscovy duck was 0. The average PIC (0.762), average h (0.7843) and average E (5.261) of the six duck populations were all high, indicating that the gene polymorphisms and genetic diversity were high. The test of Hardy-Weinberg equilibrium showed that the six populations in this study were all in Hardy-Weinberg disequilibrium. The F-statistic analysis results showed the range of FST was from 0.0205 (AJ515895) to 0.2558 (AJ515896). The mean FST was 0.0936. Phylogenetic study revealed that Peking duck (Z1 and Z4), Shaoxing duck, Cherry Valley duck and Aobaixing duck were clustered in one group, while the Muscovy duck was clustered in one group alone. The phylogenetic relationships among different populations were in accordance with their breeding history and distribution. Our data suggested that the 20 microsatellite loci were effective markers for analysis of genetic relationships among duck populations.

Keywords

References

  1. Ahlawat, S. P. S., R. K. Vijh, Bina Mishra, S. T. Bharani Kumar and M. S. Tantia. 2008. Genetic relationship in chicken breeds using molecular co-ancestry information. Asian-Aust. J. Anim. Sci. 21(1):6-10. https://doi.org/10.5713/ajas.2008.60610
  2. Aranguren-Mendez, J., M. Gomez and J. Jordana. 2002. Hierarchical analysis of genetic structure in Spanish donkey breeds using microsatellite markers. Heredity 89(3):207-211. https://doi.org/10.1038/sj.hdy.6800117
  3. Botstein, D., R. L. White, M. Skolnick and R. W. Davis. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. The American Journal of Human Genetics 32:314-331.
  4. Chang, G. B., H. Chang, X. P. Liu, W. M. Zhao, D. J. Ji, Y. J. Mao, G. M. Song and X. K. Shi. 2007. Genetic diversity of wild quail in China ascertained with microsatellite DNA markers. Asian-Aust. J. Anim. Sci. 20(12):1783-1790. https://doi.org/10.5713/ajas.2007.1783
  5. Chen, G. H., X. S. Wu, D. Q. Wang, J. Qin, S. L. Wu, Q. L. Zhou, F. Xie, R. Cheng, Q. Xu, B. Liu, X. Y. Zhang and O. Olowofeso. 2004. Cluster analysis of 12 Chinese native chicken populations using microsatellite markers. Asian-Aust. J. Anim. Sci. 17(8):1047-1052. https://doi.org/10.5713/ajas.2004.1047
  6. Chen, H. J., Y. S. Yue, X. Z. Fan, CH. SH. Zhang and L. X. Du. 2003. Analysis of genetic diversity of Shandong indigenous chicken breeds using microsatellite markers. Acta Genetica Sinica. 30(9):855-860.
  7. Chen, Y. X. and Z. H. Zuo. 2001. Study on genetic relationship and diversity in ducks by RAPD. Journal of Xiamen University (Natural Science) 40(1):141-145.
  8. Huang, Y. H., J. F. Tu, X. B. Cheng, B. Tang, X. X. Hu, Z. L. Liu, J. D. Feng, Y. K. Lou, L. Lin, K. Xu, Y. L. Zhao and N. Li. 2005. Characterization of 35 novel microsatellite DNA markers from the duck (Anas platyrhynchos) genome and cross-amplification in others birds. Genet. Sel. Evol. 37:455-472. https://doi.org/10.1186/1297-9686-37-5-455
  9. Kong, H. S., J. D. Oh, J. H. Lee, K. J. Jo, B. D. Sang, C. H. Choi, S. D. Kim, S. J. Lee, S. H. Yeon, G. J. Jeon and H. K. Lee. 2006. Genetic variation and relationships of Korean native chickens and foreign breeds using 15 microsatellite markers. Asian-Aust. J. Anim. Sci. 19(11):1546-1550. https://doi.org/10.5713/ajas.2006.1546
  10. Liu, G. Q., X. P. Jiang, J. Y. Wang and Z. Y. Wang. 2006. Correlations between heterozygosity at microsatellite loci, mean d2 and body weight in a Chinese native chicken. Asian-Aust. J. Anim. Sci. 19(12):1671-1677. https://doi.org/10.5713/ajas.2006.1671
  11. Maak, S., K. Neumann, R. Gattermann and R. Gattermann. 2000. First seven microsatellites developed for the Peking duck (Anas platyrhynchos). Anim. Genet. 31:228-241. https://doi.org/10.1046/j.1365-2052.2000.031003228.x
  12. Maak, S., K. Wimmers, S. Weigend and K. Neumann. 2003. Isolation and characterization of 18 microsatellites in the Peking duck (Anas platyrhynchos) and their application in other waterfowl species. Molecular Ecology Notes. 3:224-227. https://doi.org/10.1046/j.1471-8286.2003.00405.x
  13. Nei, M., F. Tajima and Y. Tateno. 1983. Accuracy of estimated phylogenetic trees from molecular data. J. Mol. Evol. 19:153-170. https://doi.org/10.1007/BF02300753
  14. Osman, S. A. M., M. Sekino, M. Nishibori, Y. Yamamoto and M. Tsudzuki. 2005. Genetic variability and relationship of native Japanese chicken assessed by microsatellite DNA profiling - focusing on the breeds established in Kochi prefecture, Japan. Asian-Aust. J. Anim. Sci. 18(6):755-761. https://doi.org/10.5713/ajas.2005.755
  15. Osman, S. A. M., M. Sekino, A. Nishihata, Y. Kobayashi, W. Takenaka, K. Kinoshita, T. Kuwayama, M. Nishibori, Y. Yamamoto and M. Tsudzuki. 2006. The genetic variability and relationships of Japanese and foreign chickens assessed by microsatellite DNA profiling. Asian-Aust. J. Anim. Sci. 19(10):1369-1378 https://doi.org/10.5713/ajas.2006.1369
  16. Ota, Y. 1993. DISPAN: Genetic distance and phylogenetic analysis. Pennsylvania State University. University Park, PA.
  17. Romanov, M. N. and S. Weigend. 2001. Analysis of genetic relationships between various populations of domestic and jungle fowl using microsatellite markers. Poult. Sci. 80:1057-1063 https://doi.org/10.1093/ps/80.8.1057
  18. Sambrook, J., E. F. Fritsch, T. Maniatis, D. Y. Jin and M. F. Li. 1999. (Translation) Molecular Cloning-A Laboratory Mannual (2nd edition). Beijing: Science Press.
  19. Saitou, N. and M. Nei. 1987. The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4:406-425.
  20. Stai and Hughes. 2003. Characterization of microsatellite loci in wild and domestic Muscovy ducks (Cairina moschata). Anim. Genet. 34(5):387-389.
  21. Tang, Q. P., H. F. Li, Y. J. Tu, K. W. Chen, N. Yang, Y. H. Ma and D. W. Zhao. 2007. Analysis of genetic diversity of the domestic concern duck breeds in China. Journal of Northwest Sci-Tech University of Agriculture and Forestry 35(2):47-52.
  22. Wang, J. Z., M. X. Chu, A. G. Wang, N. Li, J. L. Fu, F. Xie and G. H. Chen. 2004. Genetic relationships among seven sheep populations using four microsatellite markers. Hereditas. 26(5):637-643.
  23. Wu, CH. X., G. H. Chen, D. Q. Wang, X. Y. Zhang, K. H. Wang, R. Chen, B. Liu, Q. Xu and Q. L. Zhou. 2004. Analysis of genetic diversity among Chinese native chicken breeds using microsatellite markers. Acta Genetica Sinica. 31(1):43-50.
  24. Xiao, W. 2002. Study the genetic diversity of Chinese native pigs using blood protein polymorphism. China Agricultural University doctorate thesis.
  25. Yan, F. H., ZH. H. Zuo, M. Chen, Y. Q. Song, L. J. Lu and Y. X. Chen. 2005. Analysis of genetic diversity and relationship among some Chinese domestic ducks and wild ducks using AFLP. Journal of Xiamen University (Natural Science). 44(5): 729-733.
  26. Zhou, X. M. and G. Y. Chen. 2002. The technical manuals of efficient production duck. Shanghai, Shanghai Science and Technology Press. pp. 27-28.
  27. Zuo, Z. H., Y. X. Chen and L. J. Lu. 2004. Studies on genetic diversity and molecular marker of Jinding duck. Journal of Xiamen Universit (Natural Science) 43(2):256-259.

Cited by

  1. Blood protein polymorphism and genetic diversity in locally adapted Muscovy duck (Cairina moschata) in Nigeria vol.56, pp.2078-6344, 2015, https://doi.org/10.1017/S2078633614000526
  2. Microsatellite-based Genetic Diversity and Evolutionary Relationships of Six Dog Breeds vol.22, pp.8, 2009, https://doi.org/10.5713/ajas.2009.80493
  3. Genetic Characterization of Moti Indian Native Duck Using Microsatellite Markers vol.38, pp.2, 2008, https://doi.org/10.1080/09712119.2010.10539515
  4. Genetic Diversity Analyses of Asian Duck Populations using 24 Microsatellite Markers vol.44, pp.2, 2008, https://doi.org/10.5536/kjps.2017.44.2.75