Anatomy & Biological Anthropology (해부∙생물인류학)

Korean Association of Physical Anthropologists (대한체질인류학회)
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생쥐 배아 혀 상피의 발생과정에서 나타나는 사이토케라틴 1, 14와 PAX 9에 관한 면역조직화학적 연구

Jung, Hye-In;Jin, Myoung-Uk;Cho, Je-Yoel;Jung, Han-Sung;Kim, Jae-Young
정혜인;진명욱;조제열;정한성;김재영

  • Published : 20081200
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Abstract

Epithelial differentiation and morphogenesis in skin and oral mucosa were elucidated using various experimental tools. However, tongue epithelial differentiation has not been examined properly yet. In this study, we identified the relationship between morphological changes and localizations of differentiation markers, such as cytokeratins and PAX 9 in mice embryonic tongue development. Protective barrier formation and localization pattern of cytokeratins in tongue epithelium were examined with toluidine blue staining and immunohistochemistry respectively. Localization patterns of PAX 9 and Cytokeratin 14 were coincided during tongue epithelium development. In addition, compared with Ki67 localizations, marker for cell proliferation, localization patterns of PAX 9 and Cytokeratin 14 would suggest that these factors would involve in tongue barrier formation through cell proliferation. Based on these results, tongue epithelial differentiation woule begin at E14 with the specific localizations of PAX 9 and Cytokeratin 14 prior to protective barrier formation then Cytokeratin 1, keratinization marker, would involve in protective barrier and filiform papillae formations.

피부의 상피에 대한 연구는 부속기관의 발생 그리고 보호장벽 형성과 같은 다양한 분야에서 연구되어 왔다. 혀 상피조직의 발생과 관련된 보호장벽의 형성 및 상피조직 분화에 대해서는 많은 연구가 필요한 실정이다. 본 연구에서는 혀의 보호장벽이 형성되고 상피의 분화가 일어나는 발생중인 혀를 이용하여 보호장벽 형성과정을 면밀하게 관찰하였고, 피부의 상피에서 나타나는 다양한 사이토케라틴의 발현양상을 발생중인 혀에서 조직면역화 학방법으로 확인하였다. 혀 상피의 발생과정에서 PAX 9의 발현이 사이토케라틴 14와 겹쳐서 나타나는 것을 확인할 수 있었으며, 또한 Ki67을 이용한 상피세포의 증식양상과 비교해 볼 때, 유사하게 나타나는 것을 확인할 수 있었 는데 이는 PAX 9, 사이토케라틴 14와 세포의 증식이 혀 보호장벽 형성에 관여하는 중요한 인자로 작용할 수 있 을 것이라고 생각된다. 이러한 다양한 상피분화관련 인자들의 발현양상들을 비교해 볼 때, 혀 상피의 분화는 E15 에서 본격적으로 시작되어 보호장벽의 형성 이전에 사이토케라틴 14와 PAX 9과 같은 인자들의 발현이 먼저 나 타나게 되면서 상피의 분화가 진행되고, 이후 사이토케라틴 1과 같은 각질화 관련 인자가 보호장벽 형성에 기여한다고 생각된다.

Keywords

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