Manufacturing Process and Food Safety of Functional Food Material Containing High Level of trans-Resveratrol with Domestic Grape and Fruit Stem

국내산 포도 및 송이가지를 이용한 trans-Resveratrol 고함유 기능성 식품소재 제조공정 및 식품 안정성

Ahn, Jun-Bae
안준배

  • Published : 2008.08.01

Abstract

Trans-resveratrol, one of the functional components of grape fruit stem, has been known for its many pharmacological effects. In this study, pilot scale (1,000 kg) fermentation of domestic grape and fruit stem was carried out and fermented material containing high levels of trans-resveratrol(3.28 mg/L) and polyphenols(3.23 g/L) was obtained. Optimal concentration condition was investigated for development of manufacturing process of functional food and food additive. In case that concentration of the fermented material was carried out under 20 torr vacuum pressure, at 40℃ and for 2 hours, the loss of trans-resveratrol was minimized and percentage of residual resveratrol was 87.8%. In order to elucidate safety of the concentrated material, oral toxicity(single-dose and two-week repeated, 5,000 mg/kg BW) in rat and genotoxicity(bacterial reverse mutation, micronucleus, chromosome aberration) were tested. The concentrated material showed no toxicity and could be used as functional food and food additive.

Keywords

References

  1. Adrian M, Jeandet P, Bessis R, Joubert JM. 1996. Induction of phytoalexin(resveratrol) synthesis in grapevine leaves treated with aluminum chloride(AlCl3). J. Agric. Food Chem. 44: 1979-1981 https://doi.org/10.1021/jf950807o
  2. Ahn JB. 2006. Development of red wine containing high level of trans-resveratrol with domestic grape. Food Engineering Progress 10: 226-232
  3. Ahn JB. 2007. Properties of functionalities and storage of red wine containing high level of trans-resveratrol. Food Engineering Progress 11: 203-207
  4. Carbo N, Costelli P, Baccino FM, Lopez-Soriano FJ. 1999. Resveratrol, a matural product present in wine, decreases tumor growth in a rat tumor model. Biochem. Biophys. Res. Commun. 254: 739-743 https://doi.org/10.1006/bbrc.1998.9916
  5. Han YN, Ryu SY, Han BH. 1984. Antioxidant activity of resveratrol closely correlates with its monoamine oxidase A inhibitory activity. Arch. Pharm. Res. 32: 801-804
  6. Hascalik S, Celik O, Turkoz Y, Hascalik M, Cigremis Y, Mizrak B, Yologlu S. 2004. Resveratrol, a red wine constituent polyphenol, protects from ischemia-reperfusion damage of the ovaries. Cynecol. Obstet. Invest. 57: 218-223 https://doi.org/10.1159/000076760
  7. Hur SK, Kim SS, Heo YH, Ahn SM, Lee BG, Lee SK. 2001. Effects of the grapevine shoot extract on free radical scavenging activity and inhibition of pro-inflammatory mediator production in RAW264.7 macrophages. J. Applied Pharmacology 9: 188-193
  8. Hurh YJ, Kim JH, Seo HJ, Kong G, Surh YJ. 1999. Anticarcinogenic activity of resveratrol, a major natioxidant present in red wine : Induction of apoptosis in human cancer cells. Environ. Mut. Carc. 19: 56-62
  9. Jang M, Cai L, Udeani GO, Slowing KV, Thomas CF, Beecher CWW, Fong HHS, Farnsworth NR, Kinghorn AD, Mehta RG, Moon RC, Pezzuto JM. 1997. Cancer chemopreventive activity of resveratrol, a natural product derived form grapes. Science 275: 218-220 https://doi.org/10.1126/science.275.5297.218
  10. Jeandet P, Bessis R, Gautheron B. 1991. The production of resveratrol (3,5,4'-trihydroxystilbene) by grape berries in different developmental stages. Am. J. Enol. Vitic. 42: 41-46
  11. Kim KS, Ghim SY, Seu YB, Song BH. 1999. High level of transresveratrol, a natural anti-cancer agent, found in Korean Noul red wine. J. Microbiol. Biotechnol. 9: 691-693
  12. Langcake P, Pryce RJ. 1979. The production of resveratrol by Vitis vinifera and other memebers of the vitaceae as a response to infection or injury. Physiological Plant Pathology 9: 77-86
  13. Le Corre L, Fustier P, Chalabi N, Bignon YJ, Bernard-Gallon D. 2004. Effects of resveratrol on the expression of a panel of genes interacting with the BRCA1 oncosuppressor in human breast cell line. Clin. Chim. Acta 344: 115-121 https://doi.org/10.1016/j.cccn.2004.02.024
  14. Lee JC, Lee SM, Kim JH, Ahn SM, Lee BG, Chang IS. 2003. Protective effect of resveratrol on the oxidative stress-induced inhibition of gap junctional intercellular communication in HaCaT keratinocytes. J. Applied Pharmacology 11: 224-231
  15. Leiro J, Alvarez E, Arranz JA, Laguna R, Uriarte E, Orallo F. 2004. Effects of cis-resveratrol on inflammatory murine macrophages. J. Leukocyte Biology 75: 1156-1165 https://doi.org/10.1189/jlb.1103561
  16. Liontas A, Yeger H. 2004. Curcumin and resveratrol induce apoptosis and nuclear translocation and activation of p53 in human neuroblastoma. Anticancer Res. 24: 987-998
  17. Pace-Asciak CR, Hahn S, Diamandis EP, Soleas G, Goldberg DM. 1995. The red wine phenolics trans-resveratrol and quercetin block human platelet aggregation and eicosanoid synthesis implications for protection against coronary heart disease. Clin. Chim. Acta 235: 207-219 https://doi.org/10.1016/0009-8981(95)06045-1
  18. Paul B, Chereyathmanjiyil A, Masih I, Chapuis L, Benoit A. 1998. Biological control of Botrytis cinerea causing grey mold disease of grapevine and elicitation of stilbene phytoalexin(resveratrol) by a soil bacterium. FEMS Microbiology Letters 165: 65-70 https://doi.org/10.1111/j.1574-6968.1998.tb13128.x
  19. Pendurthi UR, Williams JT, Rao LVM. 1999. Resveratrol, a polyphenolic compound found in wine, inhibits tissue factor expression in vascular cells: A possible mechanism for the cardiovascular benefits associated with moderate consumption of wine. Arteriscler. Thromb. Vasc. Biol. 19: 419-426 https://doi.org/10.1161/01.ATV.19.2.419
  20. Renaud S, De Lorgeril M. 1992. Wine, alcohol, platelets, and the French paradox for coronary heart disease. Lancet. 339: 1523-1526 https://doi.org/10.1016/0140-6736(92)91277-F
  21. Sarig P, Zutkhi Y, Monjauze A, Lisker N, Ben-Arie R. 1997. Phytoalexin elicitation in grape berries and their susceptibility to Rhizopus stolonifer. Physiological and Molecular Plant Pathology 50: 337-347 https://doi.org/10.1006/pmpp.1997.0089
  22. Szewczuk LM, Forti L, Stivala LA, Penning TM. 2004. Resveratrol is a peroxidase-mediated inactivator of COX-1 but not COX-2. J. Biol. Chem. 279: 22729-22737