Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Comparison of Motility, Acrosome, Viability and ATP of Boar Sperm with or without Cold Shock Resistance in Liquid Semen at 17℃ and 4℃, and Frozen-thawed Semen

  • Yi, Y.J. (Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Li, Z.H. (Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Kim, E.S. (Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Song, E.S. (Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Kim, H.B. (Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Cong, P.Q. (Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Lee, J.M. (Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Park, Chang-Sik (Research Center for Transgenic Cloned Pigs, Chungnam National University)
  • Received : 2007.06.22
  • Accepted : 2007.09.03
  • Published : 2008.02.01
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Abstract

This study was designed to analyze boar sperm to compare motility, acrosome morphology, viability and ATP by various preservation methods between Duroc boar A with cold shock resistance sperm and Duroc boar B with cold shock sensitivity sperm. Semen volume, sperm concentration, motility and normal acrosome between Duroc boar A and B did not show any differences within 2 h after collection. There were no differences in sperm motility and normal acrosome between boar A and B at 1 day of preservation at $17^{\circ}C$ and $4^{\circ}C$, respectively. However, sperm motility and normal acrosome from 2 day of preservation at $17^{\circ}C$ and $4^{\circ}C$, respectively, were higher for boar A than boar B. The frozen-thawed sperm motility and normal acrosome were higher for boar A than boar B. The sperm viability and ATP concentration according to storage period of liquid semen at $17^{\circ}C$ and $4^{\circ}C$ were higher for boar A than boar B. Also, the sperm viability and ATP concentration of frozen-thawed semen were higher for boar A than boar B. In conclusion, we found out that the original quality of boar semen with cold shock resistance sperm played an important role.

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References

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