Food Engineering Progress (산업식품공학)

Korean Society for Industrial Food Engineering (한국산업식품공학회)
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A Multiplex PCR Assay for the Direct Detection of Salmonella spp., Yersinia enterocolitica, and Staphylococcus aureus in Food

식품에서 Salmonella spp., Yersinia enterocolitica 및 Staphylococcus aureus의 직접검출을 위한 Multiplex PCR

Yoon, Jang-Ho;Lee, Su-Jin;Hong, Kwang-Won
윤장호;이수진;홍광원

  • Published : 2008.03.10
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Abstract

A multiplex polymerase chain reaction method was developed for direct detection of Salmonella spp., Yersinia enterocolitica, and Staphylococcus aureus in food. Specificities of 10 primer sets for Salmonella spp.,8 primer sets for Y. enterocolitica, and 7 primer sets for S. aureus were tested against numerous strains from 51 different bacterial species using singleplex PCR. Then the three primer pairs specific to the invA gene of Salmonella spp., the ail gene of Y. enterocolitica, and the nuc gene of S. aureus were combined to established the multiplex PCR assay, and were applied to rapid detection of them in pure cultures and artificially inoculated milk. The detection limits of multiplex PCR were 1.6x103, 7.0x104, and 1.1x105 cfu/ml for Salmonella spp., Y. enterocolitica, and S. aureus, respectively, in artificially inoculated milk without enrichment. Our multiplex PCR assay is rapid and specific, and may be applicable for simultaneous detection of Salmonella spp., Y. enterocolitica, and S. aureus strains in food without enrichment step.

Keywords

References

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