Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
권호 표지

Survey and Application of DNA Makers Linked to TSWV Resistance

TSWV 저항성 파프리카 품종 개발을 위한 분자 표지의 검정

Kim, Hyoun-Joung;Yang, Hee-Bum;Chung, Bong-Nam;Kang, Byoung-Cheorl
김현정;양희범;정봉남;강병철

  • Published : 20081200
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Abstract

The incidence of Tomato spotted wilt virus (TSWV) in pepper was recently reported and TSWV has been rapidly spreading in Korea. However, TSWV-resistant pepper cultivars have not been developed in Korea so far. To develop resistant cultivars, establishment of a reliable resistance screening method is most critical. Mechanical inoculation of TSWV is known to be very inefficient due to many escapes. Hence, deployment of molecular markers linked to the TSWV resistance gene (Tsw) will greatly improve efficiency of TSWV- resistant cultivar breeding. In order to deploy TSWV resistance-linked markers in a paprika breeding program, we applied previously developed molecular markers, which include five random amplified polymorphic DNA (RAPD) and one cleaved amplified polymorphic sequence (CAPS) markers, on six resistant and 11 susceptible paprika cultivars along with original TSWV-resistant materials, Capsicum chinense PI152225 and PI159236. In addition, several other markers close to the resistance locus (Tsw) were selected by a map comparison and applied on the same materials. Except for the CAPS marker SCAC568, no markers showed consistent polymorphisms between resistant and susceptible pepper materials. SCAC568 produced polymorphisms between three resistant cultivars of Zeraim and all susceptible cultivars. When the SCAC568 marker was applied on a BCF₁ population of 92 individuals, genotypes of SCAC568 were cosegregated with phenotypes in 90 individuals out of 92. These results demonstrate that SCAC568 can be deployed in pepper breeding programs in combination with TSWV-resistant cultivars from Zeraim.

Tomato spotted wilt virus(TSWV)는 최근에 국내 고추에서 발병하여 보고된 후, 점차적으로 발생피해가 증가하고 있는 병원성이 강한 바이러스이다. 국내 고추에는 저항성 품종이 알려진 바가 없어 TSWV 저항성 품종을 육성하는 일이 주목 받고 있으나, 저항성 고추를 육종하는 과정에서 저항성과 이병성을 선별하는 TSWV 접종도 쉽지 않은 것으로 보고되고 있다. 따라서, 효과적인 저항성 개체 선발을 위한 TSWV 분자 표지의 개발이 절실하다. 지금까지 개발된 TSWV 분자 표지로는 random amplified polymorphic DNA(RAPD) 5개와 cleaved amplified polymorphic sequence(CAPS)한 개가 보고되었으나, 이들 분자 표지를 저항성 개체 선발에 활용하기 위해서는 육종집단에 적용하여 재현성과 선별 정도를 조사하는 연구가 선행되어야 한다. 따라서 본 연구는 보고된 TSWV 분자 표지와 저항성 유전자(Tsw) 근처의 conserved ortholog set II(COSII), simple sequence repeat (SSR) 분자 표지를 수집하여 TSWV 저항성 재료인 PI152225, PI159236 계통, 저항성과 이병성 파프리카 품종을 이용하여 다형성을 조사하였다. RAPD, COSII, SSR 분자 표지는 다형성 밴드가 나타나지 않았으나 CAPS 표지인 SCAC568는 Zeraim 회사의 저항성 품종과 PI152225에서 이병성과 구별되는 다형성 밴드가 증폭되었고, BCF1 집단 92주 중 90주에서 표현형과 유전형이 일치하는 결과를 보였다. SCAC568 표지는 Zeraim 품종을 재료로 하는 TSWV 저항성 파프리카 품종(Marker-assisted selection) 육종에 유용하게 활용될 것으로 판단된다.

Keywords

References

  1. Boiteux, L.S. 1995. Allelic relationships between genes for resistance to tomato spotted wilt tospovirus in Capsicum chinense. Theor. Appl. Genet. 90:146-149
  2. Black, L.L., H.A. Hobbs, and J.M.Jr.Gatti. 1991. Tomato spotted wilt virus resistance in C. chinense PI152225 and 159236. Plant Dis. 75:863
  3. Caranta, C., A. Thabuis, and A. Palloix. 1999. Development of a CAPS marker for the Pvr4 locus: A tool for pyramiding potyvirus resistance genes in pepper. Genome 42:1111-1116 https://doi.org/10.1139/gen-42-6-1111
  4. Cho, J.D., S.K. Kim, S.H. Lee, G.S. Choi, and B.N. Chung. 2007. Viruses and symptoms on peppers, and their infection in Korea. Res.Plant Dis.13:75-81 https://doi.org/10.5423/RPD.2007.13.2.075
  5. Jahn, M., I. Paran, K. Hoffmann, E.R. Radwanski, K.D. Livingstone, R.C. Grube, E. Aftergoot, M. Lapidot, and J. Moyer. 2000. Genetic mapping of the Tsw locus for resistance to the Tospovirus Tomato spotted wilt virus in Capsicum spp. and its relationship to the Sw-5 gene for resistance to the same pathogen in tomato. Mol. Plant-Microbe Interact. 13:673-682 https://doi.org/10.1094/MPMI.2000.13.6.673
  6. Kang, B.C., S.H. Nahm, J.H. Huh, H.S. Yoo, J.W. Yu, M.H. Lee, and B.D. Kim 2001. An interspecific (Capsicum annuum $\times$ C. chinense) $F_2$ linkage map in pepper using RFLP and AFLP markers. Theor. Appl. Genet. 102:531-539 https://doi.org/10.1007/s001220051678
  7. Kim, J.K., G.S. Choi, J.S. Kim, and J.K. Choi. 2004. Characterization of tomato spotted wilt virus from paprika in Korea. Plant Pathol. J. 20:297-301 https://doi.org/10.5423/PPJ.2004.20.4.297
  8. Moury, B., A. Palloix, K.G. Selassie, and G. Marchoux. 1997. Hypersensitive resistance to tomato spotted wilt virus in three Capsicum chinense accessions is controlled by a single gene and is overcome by virulent strains. Euphytica 94:45-52 https://doi.org/10.1023/A:1002997522379
  9. Moury, B., K.G. Selassie, G. Marchoux, A.-M. Daubeze, and A. Polloix. 1998. High temperature effects on hypersensitive resistance to tomato spotted wilt tospovirus (TSWV) in pepper (Capsicum chinense Jacq.). Eur. J. Plant Pathol. 104:489-498 https://doi.org/10.1023/A:1008618022144
  10. Moury, B., S. Pflieger, A. Blattes, V. Lefebvre, and A. Polloix. 2000. A CAPS marker to assist selection to tomato spotted wilt virus (TSWV) resistance in pepper. Genome 43:137-142 https://doi.org/10.1139/gen-43-1-137