Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Optimization of Extraction Condition on Fig (Ficus carica L.) by Response Surface Methodology

반응표면분석법에 의한 무화과 열수 추출조건의 최적화

  • Published : 2008.02.28
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Abstract

Response surface methodology (RSM) was applied for monitor the yields of desirable substances from fig (Ficus carica L) under different extraction conditions. The maximum yield was 66.46% at 22.08 mL/g of solvent to sample ratio, $90.59^{\circ}C$ extraction temperature and 148.04 min extraction time. The maximum total phenolics was $121.31{\mu}g/mL$ at 17.87 mL/g, $98.82^{\circ}C$, and 130.80 min. The maximum electron donating ability was 54.09% at $121.31{\mu}g/mL$, 18.13 mL/g, and $98.81^{\circ}C$. The maximum value of protease activity was 54.51 unit/min at 17.45 mL/g, $99.01^{\circ}C$, and 131.43 min. In addition, the maximum value of reducing sugar content was 19.14 mg/mL in 22.66 mL/g, $86.30^{\circ}C$, and 153.59 min. The optimum conditions estimated by RSM for maximal extraction of the effective components were $17{\sim}25$ mL/g of solvent to sample ratio, $80{\sim}100^{\circ}C$ of extraction temperature, and $100{\sim}170$ min of extraction time.

무화과를 이용한 기능성 음료의 제조를 위하여 반응표면 분석법에 의하여 무화과의 추출조건에 따른 무화과 유용성분의 추출특성을 모니터링하였다. 수율의 최대값은 66.46%이었으며, 이때의 추출조건은 시료에 대한 용매비 22.08 mL/g, 추출온도 $90.59^{\circ}C$ 및 추출시간 148.04 min이었다. 총페놀성 화합물 함량은 시료에 대한 용매비 17.87 mL/g, 추출온도 $98.82^{\circ}C$ 및 추출시간 130.80 min에서 최대 값 $121.31{\mu}g/mL$을 나타내었으며, 전자공여능은 시료에 대한 용매비 18.13 mL/g, 추출온도 $98.81^{\circ}C$ 및 추출시간 127.95 min일때 최대값을 나타내었다. 무화과 추출물의 단백분해효소의 활성은 시료에 대한 용매비 17.45mL/g, 추출온도 $99.01^{\circ}C$ 및 추출시간 131.43 min일 대 최대값을 나타내었으며, 환원당 함량은 시료에 대한 용매비 22.66 mL/g, 추출온도 $86.30^{\circ}C$ 및 추출시간 153.59 min에서 최대값이 19.14 mg/mL이었다. 무화과 유용성분의 추출을 극대화할 수 있는 최적 추출조건의 범위는 시료에 대한 용매비 $17{\sim}25$ mL/g, 추출온도 $80{\sim}100^{\circ}C$ 및 추출시간 $100{\sim}170$ min으로 예측되었으며, 실제 실험을 통하여 그 신뢰성을 검증하였다.

Keywords

References

  1. Williams, D.C., Sgarbieri, V.C. and Whitaker, J.R. (1968) Proteolytic activity in the genus Ficus. Plant Physiol., 43, 1083-1088 https://doi.org/10.1104/pp.43.7.1083
  2. Vinson, J.A. (1999) The functional food properties of figs. Cereal Food World, 44, 82-87
  3. Kim, S.S., Lee, C.H., Oh, S.L. and Chung, D.H. (1992) Chemical components in the two cultivars of Korean figs(Ficus carica L.) J. Korean Agric. Chem. Soc., 35, 51-54
  4. Jeong, M.R., Kim, B.S. and Lee, Y.E. (2002) Physicochemical characteristics and antioxidative effects of Korean figs (Ficus carica L.). J. East Asian Soc. Dietary Life, 12, 566-573
  5. Kim, K.H. (1981) Chemical components of Korean figs and its storage stability. Korean Food Sci. Technol., 13, 165-169
  6. Kim, S.S., Lee, C.H., Oh, S.L. and Chung, D.H. (1992) Chemical components in the two cultivars of Korean figs (Ficus carica L.). J. Korean Agric. Chem. Soc., 35, 51-54
  7. Kim, J.S. and Kim, J.P. (1987) Studies on the digestion of beef by ficin treatment. J. Korean Agric. Chem. Soc., 30, 210-218
  8. Kim, B.S., Jeong, M.R. and Lee, Y.E. (2003) Quality characteristics of Muhwakwa-pyun with various starches. Korean J. Soc. Food Cookery Sci., 19, 783-793
  9. Kim, K.H. (1981) Chemical components of Korean figs and its storage stability. Korean Food Sci. Technol., 13, 165-169
  10. Kang, S.G., Yoon, S.W., Kim, J.M., Kim, S.J. and Jung, S.T. (2001) Quality of accelerated salt-fermented anchovy sauce prepared with fig. J. Korean Soc. Food Sci. Nutr., 30, 1142-1146
  11. Kim, D.H. (1999) Studies on the production of vinegar from fig. J. Korean Soc. Food Sci. Nutr., 28, 53-6
  12. Hou, W.N. and Kim, M.H. (1998) Processing of low sugar jams from fig pulp treated with pectinesterase. Korean J. Food Sci. Technol., 30, 125-131
  13. Park, B.H. and Park, W.K. (1994) A study on the manufacturing of fig conserves for beef tenderizing. J. Korean Soc. Food Nutr., 23, 1027-1031
  14. Gontard, N., Guilbert, S. and Cuq, J.L. (1992) Edible wheat gluten films: Influence of the main process variables on film properties using response surface methodology. J. Food Sci., 57, 190-196 https://doi.org/10.1111/j.1365-2621.1992.tb05453.x
  15. SAS Institute, Inc. (1990) SAS User's Guide. Statistical Analysis Systems Institute, Cary, NC, USA
  16. Martha, L.A. and James, P.B. (1992) The Mathematica handbook, Compatible with Mathematica, Version 2.0 Harcourt Brace, Massachusetts : An Imprint of a Division of Academic Press
  17. Amerinem, M.A. and Ough, C.S. (1958) Method for analysis of Musts and Win. Wiley & Sons, New York, p.176-180
  18. Blois, M.S. (1958) Antioxidant determination by the use of a stable free radical. Nature, 181, 1199-1200 https://doi.org/10.1038/1811199a0
  19. Yoo, C.K., Seo, W.S., Lee, C.S. and Kang, S.M. (1998) Purification and characterization of fibrinolytic enzyme excreted by Bacillus subtilis K-54 isolated from Chunggukjang. Korean J. Appl. Microbial. Biotechnol., 26, 507-514
  20. The korea society of food science and nutrition. (2000) Handbook of experiments in food science and nutrition. Hyoil Publishing Co., Seoul, Korea, p.151-152
  21. Lee, G.D. and Kwon, J.H. (1998) The use of response surface methodology to optimize the Maillard reaction to produce melanoidins with high antioxidative and antimutagenic activities. Int. J. Food Sci. Technol., 33, 375-383 https://doi.org/10.1046/j.1365-2621.1998.00164.x
  22. Kim, N.M., Ko, S.R., Choi, K.J. and Kim, W.J. (1993) Effect of some factors on extraction of effectual components in cinnamon extracts. J. Korean Agr. Chem. Soc., 36, 17-22
  23. Yoon, O.H. and Cho, J.S. (2007) Optimization of extraction conditions for hot water extracts from Chrysanthemum indicum L. by response surface methodology. Korean J. Food Cookery Sci., 23, 1-8
  24. Lee, G.D., Kim, J.O., Joo, G.J. and Kwon, J.H. (2005) Optimum Conditions for the Extraction of Effective Substances from the Stem of Opuntia fiscus-indica. Food Sci. Biotechnol., 14, 190-195
  25. Kang, Y.H., Park, Y.K. and Lee, G.D. (1996) The nitrite scavenging and electron donating ability of phenolic compounds. Korean J. Food Sci. Technol., 28, 232-239
  26. Englund, P.T., King, T.P., Graig, L.C. and Walti, A. (1968) Studies on ficin. 1. Its islation and characterization. Biochem., 9, 163-175
  27. Kim, J.P., Suh, J.S. and Kim, J.S. (1986) Isolation and purification of ficin from fig latex (in Korea). Korean J. Food Sci. Technol., 18, 270-277
  28. Kang, C.K. and Rice, E.E. (1970) Degradation of various meat fractions by tenderizing enzymes. J. Food Sci., 35, 563-565 https://doi.org/10.1111/j.1365-2621.1970.tb04809.x