Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
권호 표지

Polyphenol Contents and Antioxidant Activity of Extracts from Angelica dahurica Root after Different Conditions of Microwave-assisted Extraction

마이크로웨이브 추출조건에 따른 백지 추출물의 폴리페놀 함량과 항산화 작용

  • Joo, Eun-Young (Department of Herbal Biotechnology, Daegu Haany University) ;
  • Kim, Nam-Woo (Department of Herbal Biotechnology, Daegu Haany University)
  • 주은영 (대구한의대학교 한방생약자원학과) ;
  • 김남우 (대구한의대학교 한방생약자원학과)
  • Published : 2008.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

This study investigated the antioxidant activities of extracts from Angelica dahurica roots after microwave-assisted extraction with different levels of energy (120, 240W) and extraction time (5, 10, 15 and 30 min). The highest extraction yield was 11.77 mg% in water at 240W for 30 min followed by 11.42 mg% in water at 120Wand 30 min. The highest total polyphenol contents was 32.36 mg/g in an ethanol extract, followed by 31.77mg/g in water extract at the same conditions of 240W, 30 min. The electron donating abilities both the ethanol extract obtained using 240W and 30 min and the water extract obtained employing 120W and 5min showed the highest values, 83.55% and 82.49% respectively at a concentration 1.0mg/mL. The highest superoxide dismutase (SOD)-like activity was 14.16% in ethanol extract at 120Wand 15min, followed by 13.22% in the water extract at 120W and 5 min. The best extraction yield and polyphenol content after microwave-assisted extraction were achieved with 240W and 30 min using water. The best condition for extraction of electron donating ability and SOD-like activity from A dahurica roots were 120W and 5 minutes using water.

백지의 유용성분을 추출하기 위한 최적의 추출조건을 알아보고자 MAE 추출장치를 이용하여 물과 에탄올을 용매로 에너지 강도와 추출시간을 달리하여 백지 추출물의 수율, 총 폴리페놀의 함량 및 전자공여능과 SOD 유사활성능을 측정하였다. 추출수율은 물을 용매로 30분간 추출한 240 W 추출물이 11.77mg%이었으며, 120W 추출물에서도 11.42 mg%의 수율을 나타내었다. 총 폴리페놀은 240W 30분간 추출된 에탄을 추출물이 32.36mg/g를, 동일한 조건의 물 추출물은 31.77mg/g의 폴리페놀을 함유하였다. 전자 공여능은 1.0 mg/mL의 농도에서 240W 30분 에탄을 추출물이 83.55%로 가장 높은 전자공여능을 나타내었으며, 그 다음으로 120W 5분 물 추출물이 82.49%의 전자공여능을 나타내었다. SOD 유사칠청능 측정 결과에서는 120W 15분 에탄을 추출물이 14.16%였으며, 120W 5분 물 추출물이 13.22%의 활성을 나타내었다. 이상의 결과 추출수율과 폴리페놀 함량이 높은 최적의 추출조건을 예측하면, 240W에서 30분간 물을 용매로 추출하는 방법이 효과적인 조건인 것으로 나타났으며, 전자공여능과 SOD유사활성 등의 추출물의 생리활성을 만족시키는 최적의 추출조건을 예측하여 보았을 때, 물을 용매로 에너지 강도는 120W에 5분간 추출하는 가장 효율적인 추출 조건인 것으로 분석되었다.

Keywords

References

  1. Kwon, J.H., Kim, K.G. and Lee, G.D. (2000) Optimization of microwave-assisted extraction under atmospheric pressure condition for soluble ginseng components. Korean J. Food Sci. Technol., 32, 117-124
  2. Pare, J.R.J., Sigoun, M. and Lapointe, J. (1991) Microwave assisted natural products extraction. US Patent 5., 002. Various international counterparts. 26, 784
  3. Giese, J. 1992. Advances in microwave food processing. Food Technol., 46, 118-123
  4. Pare, J.R.J., Belanger, M.R. and Stafford, S.S. (1994) Microwave-assisted process(MAPTm): a new tool for the analytical laboratory. Trends Anal. Chem., 13, 176-184 https://doi.org/10.1016/0165-9936(94)87033-0
  5. Schiffmann, R.F. (1992) Microwave processing in the U.S. food industry. Food Technol., 46, 50-56
  6. Lee, S.B., Lee, G.D. and Kwon, J.H. (1999) Optimization of extraction conditions for soluble ginseng components using microwave extraction system under pressure. J. Korean Soc. Food Sci. Nutr., 28, 409-416
  7. Kim, H.K., Kwon, Y.J., Kwak, H.J and Kwon, J.H. (1999) Oleoresin content and functional characteristics of fresh garlic. Korean J. Food Sci. Technol., 31, 329-335
  8. Kim, H.S. and Choi, H.J. (1990) Studies on essential oils of plants of Angelica genus in Korea(III). Kor. J. Pharmacogn., 21, 121-125
  9. 구본홍. (1994) 동의보감 한글완역본(허준 저). 대중서관, p.300, 1420
  10. Kimura, T., But, P.P.H., Guo, J.X. and Sung, C.K. (1996) International collation of traditional and folk medicine: Part 1. World scientific, Singapore, p.117-118
  11. Joo, E.Y. and Kang, W.J. (2005) Analysis on the Components of the Angelica dahurica Root. Korean J. Food Preserv., 12, 476-481
  12. Kim, S.H., Kang, S.S. and Kim, C.M. (1992) Coumarin glycosides from the roots of Angelica dahurica. Arch. Pharm. Res., 15, 73-77 https://doi.org/10.1007/BF02973988
  13. Kwon, Y.S. and Kim, C.M. (1992) Coumarin glycosides from the roots of Angelica dahurica. Kor. J. Pharmacogn., 24, 221-224
  14. Baek, N.I., Ahn, E.M., Kim, H.Y. and Park, Y.D. (2000) Furanocoumarins from the root of Angelica dahurica. Arch. Pharm. Res., 23, 467-470 https://doi.org/10.1007/BF02976574
  15. Ryu, S.Y., Kim, J.C., Kim, Y,S., Kim, H.T., Kim, W.K., Choi, G.J., Kim, J.S., Lee, S.W., Heor, J.H. and Cho, K.Y. (2001) Antifungal activities of coumarins isolated from Angelica gigas and Angelica dahurica against plant pathogenic fungi. Kor. J. Pestic. Sci., 5, 26-35
  16. Wang, N.H., Yoshiazaki, K. and Baba, K. (2001) Seven new bifuranocoumarins, dahuribirin A-G, from Japanese Bai Zh. Chem. Pharm. Bull., 49, 1085-1088 https://doi.org/10.1248/cpb.49.1085
  17. Jin, M.H., Jung, M.H., Lim, Y.H., Lee, S.H., Kang, S.J. and Cho, W.G. (2004) Promoting synthesis of collagen from Angelica dahurica root. Kor. J. Pharmacogn., 35, 315-319
  18. Shin, K.H., Kim, O.N. and Woo, W.S. (1988) Effect of the constituents of Angelica dahurica Radix on hepatic drug metabolizing enzyme activity. Kor. J. Pharmacogn., 19, 19-27
  19. Kim, D.K., Lim, J.P., Yang, J.H., Eom, D.O., Eun, J.S. and Leem, K.H. (2002) Acetylcholinesterase inhibitors from the roots of Angelica dahurica. Arch. Pharm. Res., 25, 856-859 https://doi.org/10.1007/BF02977004
  20. Kim, C.M., Kwon, Y.S. and Choi, S.Y. (1995) Antithrombotic effect of the BuOH soluble fraction of Angelica dahurica root. Kor. J. Pharmacogn., 26, 74-77
  21. Pae, H.O., Oh, H.C., Yun, Y.G., Oh, G.S., Jang, S.I., Hwang, K.M., Kwon, T.O., Lee, H.S. and Chung, H.T. (2002) Imperatorin, a furanocoumarin from Angelica dahurica (Umbelliferae), induces cytochrome c-dependent apoptosis in human promyelocytic leukaemia, HL-60 cells. Pharmacol. Toxicol., 91, 40-48 https://doi.org/10.1034/j.1600-0773.2002.910107.x
  22. Lechner, D., Stavri, M., Oluwatuyi, M., Pereda-Miranda, R. and Gibbons, S. (2004) The anti-staphylococcal activity of Angelica dahurica. Phytochemistry, 65, 331-335 https://doi.org/10.1016/j.phytochem.2003.11.010
  23. Schinella, G.R., Tournier, H.A., Prieto, J.M., Rios, J.L., Buschiazzo, H. and Zaidenberg, A. (2002) Inhibition of Trypanosoma cruzi growth by medical plant extracts. Fitoterapia, 73, 569-575 https://doi.org/10.1016/S0367-326X(02)00246-0
  24. Lee, Y.S., Jang, S.M. and Kim, N.W. (2007) Antioxidative activity and physiological function of the Angelica dahurica roots. J. Korean Soc. Food Sci. Nutr., 36, 20-26 https://doi.org/10.3746/jkfn.2007.36.1.020
  25. AOAC. (2005) Official method of analysis. 18th ed., Association of official analytical chemists. Washington D.C. USA. Chapter, 45, 21-22
  26. Blois, M.S. (1958) Antioxidant determination by the use of a stable free radical. Nature, 181, 1199-1200 https://doi.org/10.1038/1811199a0
  27. Marklund, S. and Marklund, G. (1975) Involvement of superoxide amino radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem., 47, 468-474
  28. Kim, M.H., Kim, M.C., Park, J.S., Kim, J.W. and Lee, J.O. (2001) The antioxidative effects of the water-soluble extracts of plants used as tea materials. Korean J. Food Sci. Technol., 33, 12-18
  29. Moon, J.S., Kim, S.J. and Park, Y.M. (2004) Activities of antioxidation and alcohol dehydrogenase inhibition of methanol extracts from some medicinal herbs. Korean J. Food Preserv., 11, 201-206
  30. Kim, H.J., Kwon, Y.J., Kim Y.E. and Nahmgung, B. (2004) Changes of total polyphenol content and antioxidant activity of Aster scaber Thunb extracts with different microwave-assisted extraction conditions. Korean J. Food Preserv., 11, 88-93
  31. Lim, J.D., Yu, C.Y., Kim, M.J., Yun, S.J., Lee, S.J., Kim, N.Y. and Chung, I.M. (2004) Comparison of SOD activity and phenolic compound contents in various Korean medicinal plants. Korean J. Medicinal Crop Sci., 12, 191-202