Molecular & Cellular Toxicology

The Korean Society of Toxicogenomics and Toxicoproteomics (대한독성유전 단백체학회)
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Application of Nanotechnology to Korean Black-Red Ginseng: Solubility Enhancement by Particle Size Reduction

  • Park, Seul-Ki (Department of Medical Biotechnology, SoonChunHyang University) ;
  • Kim, Yoon-Kyung (Department of Medical Biotechnology, SoonChunHyang University) ;
  • Youn, Hyung-Sun (Department of Biomedical Laboratory Science, SoonChunHyang University) ;
  • Lee, Mi-Young (Department of Medical Biotechnology, SoonChunHyang University)
  • Published : 2008.03.31
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Abstract

In order to investigate whether the particles reduced to almost nano grade might affect the chemical and physical properties of organic materials, whole Korean Black-Red Ginseng was pulverized into almost nano size and then ginsenosides, minerals, carbohydrates, lipids and proteins in the ultrafine particles were compared with those in the regular particles as control. The mean size of the ultrafine particles was in the 350 nm range, while that of the regular particles was $127{\mu}m$. More ginsenosides, minerals, carbohydrates, lipids and proteins were detected in the ultrafine particles than in the regular particles. Interestingly, more lipids from the ultrafine particles dissolved in the water than those from the regular particles in the ethanol. Absorption and transport of carbohydrate, lipid or antioxidant activity across the intestinal wall using everted intestine sacks of mice was also enhanced by particle size reduction at the almost nano scale. More cytotoxic effect against hepatoma cell growth by ultrafine particles was also found. These results could be used as the basic data for the understanding and evaluation of the effects of organic nanomaterials on the human health.

Keywords

References

  1. Moore, R. Nanomedicine and risk: further perspectives. Med Device Technol 18:28-29 (2007)
  2. Moore, R. Medical nanotechnology: evolving appropriate risk tools. Med Device Technol 18:36-37 (2007)
  3. Lanone, S. & Boczkowski, J. Biomedical applications and potential health risks of nanomaterials: molecular mechanisms. Curr Mol Med 6:651-663 (2006) https://doi.org/10.2174/156652406778195026
  4. Robichaud, C. O., Tanzil, D., Weilenmann, U. & Wiesner, M. R. Relative risk analysis of several manufactured nanomaterials: an insurance industry context. Environ Sci Technol 39:8985-8994 (2005) https://doi.org/10.1021/es0506509
  5. Bowman, D. M. & Fitzharris, M. Too small for concern? Public health and nanotechnology. Aust N Z J Public Health 31:382-384 (2007) https://doi.org/10.1111/j.1753-6405.2007.00092.x
  6. Wu, J. Z. & Ho, P. C. Evaluation of the in vitro activity and in vivo bioavailability of realgar nanoparticles prepared by cryo-grinding. Eur J Pharm Sci 29:35-44 (2006) https://doi.org/10.1016/j.ejps.2006.05.002
  7. Ruenraroengsak, P. et al. Cell uptake, cytoplasmic diffusion and nuclear access of a 6.5 nm diameter dendrimer. Int J Pharm 331:215-219 (2007) https://doi.org/10.1016/j.ijpharm.2006.12.012
  8. Patra, A. et al. Tuning the size and optical properties in molecular nano/microcrystals: manifestation of hierarchical interactions. Small 2:650-659 (2006) https://doi.org/10.1002/smll.200500385
  9. Hecq, J. et al. Preparation and in vitro/in vivo evaluation of nano-sized crystals for dissolution rate enhancement of ucb-35440-3, a highly dosed poorly water-soluble weak base. Eur J Pharm Biopharm 64: 360-368 (2006) https://doi.org/10.1016/j.ejpb.2006.05.008
  10. Hart, J. et al. Nano-sized hybrid capsules based on cyclodextrin esters: a totally new fluidic organization for penetration enhancement of cosmetic active compounds. J Cosmet Sci 57:185-186 (2006)
  11. Hop, C. E. Use of nano-electrospray for metabolite identification and quantitative absorption, distribution, metabolism and excretion studies. Curr Drug Metab 7:557-563 (2006) https://doi.org/10.2174/138920006777697909
  12. Wu, J. Z. & Ho, P. C. Evaluation of the in vitro activity and in vivo bioavailability of realgar nanoparticles prepared by cryo-grinding. Eur J Pharm Sci 29:35-44 (2006) https://doi.org/10.1016/j.ejps.2006.05.002
  13. Kanarek, M. S. Nanomaterial health effects part 3: conclusion-hazardous issues and the precautionary principle. Wis Med J 106:16-19 (2007)
  14. Feron, V. J. & Groten, J. P. Toxicological evaluation of chemical mixtures. Food Chem Toxicol 40:825-839 (2002) https://doi.org/10.1016/S0278-6915(02)00021-2
  15. Reddy, K. M. et al. Selective toxicity of zinc oxide nanoparticles to prokaryotic and eukaryotic systems. Appl Phys Lett 90:2139021-2139023 (2007)
  16. Foged, C. & Nielsen, H. M. Cell-penetrating peptides for drug delivery across membrane barriers. Expert Opin Drug Deliv 5:105-117 (2008) https://doi.org/10.1517/17425247.5.1.105
  17. Damge, C., Reis, C. P. & Maincent, P. Nanoparticle strategies for the oral delivery of insulin. Expert Opin Drug Deliv 5:45-68 (2008) https://doi.org/10.1517/17425247.5.1.45
  18. Adams, L. K., Lyon, D. Y., McIntosh, A. & Alvarez, P. J. Comparative toxicity of nano-scale $TiO_2$, $SiO_2$ and ZnO water suspensions. Water Sci Technol 54:327-334 (2006) https://doi.org/10.2166/wst.2006.891
  19. Sokolova, V. & Epple, M. Inorganic nanoparticles as carriers of nucleic acids into cells. Angew Chem Int Ed Engl (2007). [Epub ahead of print]
  20. Chan, K. et al. The effects of sinomenine on intestinal absorption of paeoniflorin by the everted rat gut sac model. J Ethnopharmacol 103:425-432 (2006) https://doi.org/10.1016/j.jep.2005.08.020
  21. Ozer, N. et al. Simple, high-yield purification of xanthine oxidase from bovine milk. J Biochem Biophys Methos 39:153-159 (1999) https://doi.org/10.1016/S0165-022X(99)00012-3
  22. Tetley, T. D. Health effects of nanomaterials. Biochem Soc Trans 35:527-531 (2007) https://doi.org/10.1042/BST0350527
  23. Malik, D. K. et al. Recent advances in protein and peptide drug delivery systems. Curr Drug Deliv 4:141-151 (2007) https://doi.org/10.2174/156720107780362339
  24. Hart, J. et al. Nano-sized hybrid capsules based on cyclodextrin esters: a totally new fluidic organization for penetration enhancement of cosmetic active compounds. J Cosmet Sci 57:185-186 (2006)
  25. Tetley, T. D. Health effects of nanomaterials. Biochem Soc Trans 35:527-531 (2007) https://doi.org/10.1042/BST0350527
  26. Sheehan, D. The potential of proteomics for providing new insights into environmental impacts on human health. Rev Environ Health 22:175-194 (2007)
  27. Sayes, C. M., Reed, K. L. & Warheit, D. B. Assessing toxicity of fine and nanoparticles: comparing in vitro measurements to in vivo pulmonary toxicity profiles. Toxicol Sci 97:163-180 (2007) https://doi.org/10.1093/toxsci/kfm018
  28. Robichaud, C. O., Tanzil, D., Weilenmann, U. & Wiesner, M. R. Relative risk analysis of several manufactured nanomaterials: an insurance industry context. Environ Sci Technol 39:8985-8994 (2005) https://doi.org/10.1021/es0506509
  29. Ruenraroengsak, P. et al. Cell uptake, cytoplasmic diffusion and nuclear access of a 6.5 nm diameter dendrimer. Int J Pharm 331:215-219 (2007) https://doi.org/10.1016/j.ijpharm.2006.12.012
  30. Jain, K. K. Nanobiotechnology-based drug delivery to the central nervous system. Neurodegener Dis 4:287-291 (2007) https://doi.org/10.1159/000101884
  31. Bhavsar, M. D. & Amiji, M. M. Polymeric nano- and microparticle technologies for oral gene delivery. Expert Opin Drug Deliv 4:197-213 (2007) https://doi.org/10.1517/17425247.4.3.197
  32. Lee, J. H. et al. Effects of ginsenosides, active ingredients of Panax ginseng, on development, growth, and life span of Caenorhabditis elegans. Biol Pharm Bull 11:2126-2134 (2007)
  33. Ko, S. R. et al. Marked production of ginsenosides Rd, F2, Rg3, and compound K by enzymatic method. Chem Pharm Bull (Tokyo) 55:1522-1527 (2007) https://doi.org/10.1248/cpb.55.1522
  34. Li, X. et al. Pharmacokinetic and absolute bioavailability study of total panax notoginsenoside, a typical multiple constituent traditional chinese medicine (TCM) in rats. Biol Pharm Bull 30:847-851 (2007) https://doi.org/10.1248/bpb.30.847
  35. Chen, X. M., Zhu, J. B., Sun, W. D. & Zhang, L. J. Effect of absorption enhancers on nasal ginsenoside Rg1 delivery and its nasal ciliotoxicity. Yao Xue Xue Bao 41:149-155 (2006)
  36. Wang, C. Z., Wu, J. A., McEntee, E. & Yuan, C. S. Saponins composition in American ginseng leaf and berry assayed by high-performance liquid chromatography. J Agric Food Chem 54:2261-2266 (2006) https://doi.org/10.1021/jf052993w
  37. Dubois, M. Colormetric method for determination of sugars and related substances. Anal Chem 28:350-356 (1956) https://doi.org/10.1021/ac60111a017
  38. Bradford, M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254 (1976) https://doi.org/10.1016/0003-2697(76)90527-3
  39. Frings, C. S., Fendley, T. W., Dunn, R. T. & Queen, C. A. Improved determination of total serum lipids by the sulfo-phospho-vanillin reaction. Clin Chem 18:673-674 (1972)