KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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The Optimization of Biohydrogen Production Medium by Dark Fermentation with Enterobacter aerogenes

Enterobacter aerogenes의 혐기발효에 의한 바이오 수소 생산 배지의 최적화

  • Kim, Kyu-Ho (Department of Chemical Engineering, University of Seoul) ;
  • Choi, Young-Jin (Department of Chemical Engineering, University of Seoul) ;
  • Kim, Eui-Yong (Department of Chemical Engineering, University of Seoul)
  • 김규호 (서울시립대학교 화학공학과) ;
  • 최영진 (서울시립대학교 화학공학과) ;
  • 김의용 (서울시립대학교 화학공학과)
  • Published : 2008.02.29
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Abstract

Hydrogen is considered as an energy source for the future due to its environmentally friendly use in fuel cells. A promising way is the biological production of hydrogen by fermentation. In this study, the optimization of medium conditions which maximize hydrogen production from Enterobacter aerogenes KCCM 40146 were determined. As a result, the maximum attainable cumulative volume of hydrogen was 431 $m{\ell}$ under the conditions of 0.5M potassium phosphate buffer, pH 6.5 medium containing 30 g/L glucose. The best nitrogen sources were peptone and tryptone for the cell growth as well as hydrogen production. The control of cell growth rate was found to be a important experimental parameter for effective hydrogen production

수소는 연료전지와 같은 친환경적인 용도로 인해 미래의 에너지로서 주목받고 있는데, 생물학적인 발효법은 수소의 생산을 위한 유망한 방법이다. 본 연구에서는 Enterobacter aerogenes KCCM 40146을 대상으로 수소 생산을 최대로 하기 위한 배지의 조건을 최적화하였다. 그 결과, 0.5M potassium phosphate buffer pH 6.5에서 glucose 30 g/L일 때 수소의 누적 농도가 431 $m{\ell}$로 최대값을 얻을 수 있었다. 질소원으로 peptone과 tryptone을 넣은 배지가 수소의 생산 뿐 아니라 균주의 성장에 가장 효율적이었다 한편, 미생물의 성장속도조절이 수소의 효율적 생산을 위해 중요한 실험변수임을 알 수 있었다.

Keywords

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