DOI QR코드

DOI QR Code

Trimethoprim Resistance of Vibrio parahaemolyticus Isolated from the Fish Farm

어류 양식장에서 분리한 Vibrio parahaemolyticus의 trimethoprim 내성

  • 오은경 (국립수산과학원 식품안전연구과) ;
  • 유홍식 (국립수산과학원 식품안전연구과) ;
  • 신순범 (국립수산과학원 식품안전연구과) ;
  • 손광태 (국립수산과학원 남해수산연구소) ;
  • 박큰바위 (국립수산과학원 남해수산연구소) ;
  • 권지영 (국립수산과학원 남해수산연구소) ;
  • 이태식 (국립수산과학원 양식환경연구소) ;
  • 이희정 (국립수산과학원 식품안전연구과)
  • Published : 2008.10.31

Abstract

Vibrio parahaemolyticus species, which cause acute gastroenteritis in humans, were isolated from farmed fish and seawater and their antimicrobial-resistance pattern and factor were investigated. They exhibited the highest resistance to ampicillin (88.9%), followed by trimethoprim (51.9%) and rifampin (22.2%). The relatively high resistance to trimethoprim was unexpected because trimethoprim was not commonly used in fish farming in Korea. R plasmid related resistance was identified by the treatment of novobiocin (7 ug/mL) and it was named as pVPBW1. A putative trimethoprim resistance gene in 2.0 kb fragment of pVPBW1 was also confirmed.

Keywords

References

  1. Bell, G.R., G.S. Traveler and C. Dworschak. 1988. De- velopment in vitro and pathogenicity of an erythro- mycin-resistant strain of Renibacterium salmonina- rum, the causative agent of bacterial kidney disease in salmonids. Dis. Aquat. Org., 4, 19-25 https://doi.org/10.3354/dao004019
  2. Birnboim, I.C. and J.A. Doly. 1979. rapid alkaline extrac- tion procedure for screening recombinant plasmid DNA. Nucl. Acids. Reser., 7, 1513-1523 https://doi.org/10.1093/nar/7.6.1513
  3. Burdeska, A. 1990. Trimethoprim-resistance in staphylo- cocci: genetic and biochemical characterisation of plasmid-encoded trimethoprim-resistant dihydro- folate reductases. Ph.D. Thesis. University of Basel, Switzerland
  4. Charpentier, E., G. Gerbaud, C. Jacquet, J. Rocourt and P. Courvalin. 1995. Incidence of antibiotic resistance in Listeria species. J. Infect. Dif., 172, 277-281 https://doi.org/10.1093/infdis/172.1.277
  5. Dalsgaard, A., A. Forslund, A. Petersen, D.J. Brown, F. Dias, S. Monteiro, K. Molbak, P. Aaby, A. Rodrigues and A. Sandstrom. 2000. Class 1 integronborne, multiple-antibiotic resistance encoded by a 150- Kilobase conjugative plasmid in epidemin Vibrio cholerae O1 strains isolated in Guinea-bissau. J. Clin. Microbiol., 38, 3774-3779
  6. Deepanjali, A., H.S. Kumar, I. Karunasagar and I. Karunasagar. 2005. Seasonal variation in abundance of total and pathogenic Vibrio parahaemolyticus bacteria in oysters along the southwest coast of India. Appl. Environ. Microbiol., 71, 3275-3580
  7. Elliot, E.L., C.A. Kaysner and M.L. Tamplin. 1982. Vibrio cholerae, V. parahaemolyticus, V. vulnificus and other Vibrio spp. In: Food and Drug Administration, Bacteriological Analytical Manual, 7th ed. AOAC international, Arlington, 111-140
  8. Glenn Rhodes, Geert Huys, Jean Swings, Patrick Mcgann, Maura Hiney, Peter Smith and Roger W. Pickup. 2000. Distribution of oxytetracycline resistance plas- mids between aeromonads in hospital and aquaculture environments: implication of Tn1721 in dissemination of the tetracycline resistance determinant tet A. Appl. Environ. Microbiol., Sept., 3883-3890
  9. Goldstein, F.W. and J. Acar. 1991. Testing Staphylococcus aureus for resistance to beta-lactams. J. Antimicrob. chemother., 26, 269-278 https://doi.org/10.1093/jac/26.2.269
  10. Ham, H.J., Y.H. JIn and Y.T. Jung. 2002. Distribution of Vibrio haemolyticus in fishery product, sold at Garak Wholesale Market and serological charac- teristics of isolated strains. J. Fd. Hyg. Saety, 17. 152-156
  11. Heo, J.H., M.H. Hung, M.H. Cho, G.H. Kim, K.C. Lee, J.H. Kim and T.S. Jung. 2002. The study on fish disease with reference to bacterial susceptibility to antibiotics in the southern area of Kyeognam. J. Vet. Clin., 19, 19-22
  12. Huovinen, P. 2001. Resistance to trimethoprim and sulfonamides. Clin. Infect. Dis., 32, 1608-1614 https://doi.org/10.1086/320532
  13. Janda, J.M., C. Powers, R.G. Bryant and S.L. Abbot. 1988. Current perspectives on the pidemiology and patho- genesis of clinically significant Vibrio ssp. Clinical microbiology Reviews, 1, 245-267 https://doi.org/10.1128/CMR.1.3.245
  14. Jeong, H.D. and S.K. Chun. 2002. The utilization of antibiotics and the treatment of bacterial diseases in fish. J. Fish. Pathol., 5, 37-48
  15. Kehrenberg, C. and S. Schwarz. 2005. dfrA20, A novel trimethoprim resistance gene from Pasteurella multocida. Antimicrob. Agents. Chemother., 49, 414-417 https://doi.org/10.1128/AAC.49.1.414-417.2005
  16. Li, J., J. Yie, W. Fu, R.W. Foo, Y. Hu, N.Y. Woo and H. Xu. 1999. Antibiotic resistance and plasmid pro- files of Vibrio isolates from cultured Sparus sarba. Wei. Sheng. Wu. Xue. Bao., 39. 461-468
  17. Maniatis, T., E.F. Fritsch and J. Sambrook. 1982. Mole- cular cloning. In: Cold Spring Habor Laboratory, 250-251
  18. Navia, M.M., J. Ruiz and J. Vila. 2004. Molecular charac- terization of the integrons in Shigella strains isolated from patients with traveler's diarrhea. Diagn. Micro- biol. Infect. Dis., 48, 175-179 https://doi.org/10.1016/j.diagmicrobio.2003.10.007
  19. Oliver, J.D. 1989. Vivrio vulinificus. In: Foodborne Bac- terial Pathogen. Doyle, M.P., ed., Marcel Dekker, inc., New York, 569-600
  20. Petersen, A., J.S. Andersen, T. Kaewmak, T. Somsiri and A. Dalsgaard. 2002. Impact of integrated fish farming on antimicrobial resistance in a pond environment. Appl. Environ. Microbiol., 68, 6036-6042 https://doi.org/10.1128/AEM.68.12.6036-6042.2002
  21. Roque, A., A. Molina-Aja, C. Bolan-Mejia and B. Gomez- Gil. 2001. In vitro susceptibility to 15 antibiotics of Vibrios isolated from penaeid shtomps in North- western Mexico. Int. Antimicrob. Agents., 17. 383- 387 https://doi.org/10.1016/S0924-8579(01)00308-9
  22. Smith, P., J. Donlon, R. Coyne and D.J. Cazabon. 1994. Fate of oxytetracyline in a fresh water fish farm: influence of effluent treatment systems. Aquaculture, 120, 319-325 https://doi.org/10.1016/0044-8486(94)90088-4
  23. Son, K.T., E.G. OH, T.S. Lee, H.J. Lee, P.H. Kim and J.H. Kim. 2005. Antimicrobial Susceptibility of Vibrio parahaemolyticus and Vibrio alginoluticus from Fish Farms on the Southern Coast of Korea. J. Kor. Fish. Soc., 38, 365-371 https://doi.org/10.5657/kfas.2005.38.6.365
  24. Thungapathra, M., K. Amita, K. Sinha, S.R. Chaudhuri, P. Garg, T. Ramamurthy, G.B. Nair and A. Ghosh. 2002. Occurrence of antibiotic resistance gene cassettes aac (6')-Ib, dfrA5, dfrA12, and ereA2 in class I integrons in non-O1, non-O139 Vibrio cholerae strains in India. Antimicrob. Agents. Chemother., 46, 2948-2955 https://doi.org/10.1128/AAC.46.9.2948-2955.2002
  25. Waldor, M.K., H. Tschape and J.J. Mekalanos. 1996. A new type of conjugative transposon encodes resistance to sulfamethoxazole, trimethoprim, and streptomycin in Vibrio cholerae O139. J. Bacteriol., 178, 4157- 4165 https://doi.org/10.1128/jb.178.14.4157-4165.1996
  26. Warren, J.W., E. Abrutyn, J.R. Hebel, J.R. Johnson, A.J. Schaeffer and W.E. Stamm. 1999. Guidelines for antimicrobial treatment of uncomplicated acute- bacterial cystitis and acute pyelonephritis in women. Clin. Infect. Dis., 29, 745-758 https://doi.org/10.1086/520427

Cited by

  1. Antibiotic-Resistance Profiles and the Identification of the Ampicillin-Resistance Gene of Vibrio parahaemolyticus Isolated from Seawater vol.43, pp.6, 2010, https://doi.org/10.5657/kfas.2010.43.6.637
  2. Antimicrobial-resistance Profiles and Virulence Genes of Vibrio parahaemolyticus Isolated from Seawater in the Wando Area vol.47, pp.3, 2014, https://doi.org/10.5657/KFAS.2014.0220
  3. Multiplex Polymerase Chain Reaction(PCR)법을 이용한 Staphylococcus aureus, Salmonella enterica subsp., Vibrio parahaemolyticus의 다중동시검출 vol.39, pp.4, 2010, https://doi.org/10.3746/jkfn.2010.39.4.595