DOI QR코드

DOI QR Code

Effects of Lipolysis and Fatty Acid Composition on Off-flavor in Goat Milk

산양유의 지방분해 특성과 지방산 조성이 산양유 이취에 미치는 영향

  • Lim, Y.S. (National Institute of Animal Science, RDA) ;
  • Ham, J.S. (National Institute of Animal Science, RDA) ;
  • Jeong, S.G. (National Institute of Animal Science, RDA) ;
  • Ahn, J.N. (National Institute of Animal Science, RDA) ;
  • Choi, S.H. (Division of Animal Science and Life Resources, Sangji University)
  • Published : 2008.02.01

Abstract

The average milk fat content in goat milk was 3.88% on yearly basis. The milk fat content of 3.8% during summer season was lower than 4.2% during winter season. Total solid content increased in proportion to milk fat. When goat milk was stored at 4℃ for 24 hr, short-chain FFA(C4:0~C10:0) and medium- and long-chain FFA(C12:0~C18:1) increased about 106% and 203%, respectively. Induced lipolysis of goat milk by homogenization increased short-chain FFA and medium- and long-chain FFA by 22% and 199%. When goat milk was treated with calf lipase, there was increase of short-chain FFA by 9 times greater than increase of medium- and long-chain FFA by 5.6 times. Treatment with lipases from Candida rugosa and Pseudomonas fluorescens resulted in increase of medium- and long-chain FFA by 34 and 162 times, respectively, which was greater than increase of short-chain FFA by 6 and 14 times, respectively. Lipolysis in goat milk stored at 4℃ for 24 hr was correlated with LPL activity in goat milk(r=0.5635). Off-flavor of goat milk was correlated with LPL activity(r=0.5777). Milk fat content was negatively correlated with LPL activity(r=-0.4627). Palmitic acid content in goat milk was correlated with off-flavor(r=0.7226).

산양유의 비유기간 중 연평균 유지방 함량이 3.88% 이었으며, 동절기(4.2%) 보다 하절기 (3.8%)에 낮았다. 유지방 함유율이 높을수록 총고형분 함량도 비례하여 증가하였다. 산양유 원유를 24시간 냉장저장 하는 동안에 저급 유리지방산(C4:0~C10:0)은 106% 증가하였으며 중급 및 고급 유리지방산(C12:0~C18:1)은 203%로 높은 증가율을 보였다. 균질한 시유에 원유를 첨가하여 반응할 때에 저급 유리지방산은 22% 증가되었으며 중급 및 고급 유리지방산은 199% 증가되었다. Lipase의 종류에 따른 지방분해실험에서 calf lipase는 중급 및 고급지방산 9배 보다 저급지방산은 5.6배로 증가시켰다. 그러나 Candida rugosa lipase와 Pseudomonas fluorescens lipase는 저급 유리지방산이 각각 6배와 14배 증가하고, 중급 및 고급 유리지방산은 각각 34배와 162배 증가하여 중급 및 고급지방산을 더 잘 분해하는 것으로 나타났다. 산양유의 LPL 활성이 높을수록 유리지방산 생성량도 증가되어 유의적 상관관계(r=0.5635)를 보였으며 또한 산양유 이취가 높아지는 상관관계(r=0.5777)를 보였다. 유지방 함량이 낮을수록 LPL 활성은 높아지는 유의적 역 상관관계(r=-0.4627)를 보였다. 산양유의 palmitic acid 함량이 높을 경우 산양유 이취 강도가 증가하는 유의적 상관관계(r=0.7226)를 보였다.

Keywords

References

  1. Alonso, L., Fontecha, J., Lozada, L, Fraga, M. J. and Juarez, M. 1999. Fatty acid composition of caprine milk: major, branched-chain, and trans fatty acids. J. Dairy Sci. 82:878-884 https://doi.org/10.3168/jds.S0022-0302(99)75306-3
  2. Arnold, R. G., Shahani, K. M. and Dwivedi, B. K. 1975. Application of lipolytic enzymes to flavor development in dairy products. J. Dairy Sci. 58:1127-1143 https://doi.org/10.3168/jds.S0022-0302(75)84692-3
  3. Astrup, H. N., Steine, T. A. and Robstad, A. M. 1985. Taste, free fatty acids and fatty acid content in goat milk. Acta Agric. Scand. 35:315-320 https://doi.org/10.1080/00015128509435788
  4. Belanger, J. 1992. Raising milk goats the modern way. A garden way publishing classic. United States. pp. 5-17
  5. Cartier, P. and Chilliard, Y. 1986. Effects of different skim milk fractions on activity of cow milk purified lipoprotein lipase. J. Dairy Sci. 69: 951-955 https://doi.org/10.3168/jds.S0022-0302(86)80486-6
  6. Chilliard, Y. 1982. Variations physiologiques des activites lipasiques et de la lipolyse spontanee dans les laits de vache, de chevre et de femme: revue bibliographique. Lait. 62:1-31 and 126-154 https://doi.org/10.1051/lait:1982611-6121
  7. Chilliard, Y., Selselet-Attou, G., Bas, P. and Morand-Fehr, P. 1984. Characteristics of lipolytic system in goat milk. J. Dairy Sci. 67:2216-2223 https://doi.org/10.3168/jds.S0022-0302(84)81569-6
  8. Chilliard, Y. and Bocquier, F. 1993. Effects of fat supplementation on milk yield and composition in dairy goats and ewes. Proc. 5th Int. Symp. "La qualita nell produzioni dei piccoli ruminanti". (Camera di Commercio Industria Artigianato Agri- cultura di Varese, Italia, 3 Dicembre 1993) 61-78
  9. Chilliard, Y., Chabosseau, J. M., Rouel, J., Capitan, P., Gominard, C., Gaborit, P., Juaneda, P. and Ferlay, A. 2002. Interactions between forage nature and sunflower or linseed oil supplement- ation on goat milk fatty acids of interest for human nutrition. Multi-Function Grasslands: Quality Forages, Animal Products and Landscapes. Durand, J. L., Emile, J. C., Huyghe, C. and Lemaire, eds. Proc. 19th Gen. Mtg. of the Eur. Grassl. Fed. La Rochelle, France, 27-30 May 2002, Grassl. Sci. Eur. 7:548-549
  10. Chilliard, Y., Ferlay, A., Rouel, J. and Lamberet, G. 2003. A review of nutritional and physiological factors affecting goat milk lipid synthesis and lipolysis. J. Dairy Sci. 86:1751-1770 https://doi.org/10.3168/jds.S0022-0302(03)73761-8
  11. Chilliard, Y. and Ferlay, A. 2004. Dietary lipids and forages interactions on cow and goat milk fatty acid composition and sensory properties. Reprod. Nutr. Dev. 44:467-492 https://doi.org/10.1051/rnd:2004052
  12. Deeth, H. C., Fitz-Gerald, C. H. and Snow, A. J. 1983. A gas chromatographic method for the quantitative determination of free fatty acids in milk and milk products. New Zealand J. Dairy Sci. Technol. 18:13-20
  13. Feng, S., Lock, A. L. and Garnsworthy, P. C. 2004. A rapid method for determining fatty acid composition of milk. J. Dairy Sci. 87:3785-3788 https://doi.org/10.3168/jds.S0022-0302(04)73517-1
  14. Glass, R. L., Troolin, H. A. and Jenness, R. 1967. Comparative biochemical studies of milks. IV. Constituent fatty acids of milk fats. Comp. Biochem. Physiol. 22:415-425 https://doi.org/10.1016/0010-406X(67)90604-4
  15. Ha, J. K. and Lindsay, R. C. 1990. Method for analysis of free and total volatile branched-chain fatty acids in cheese and milk fat. J. Dairy Sci. 73:1988-1999 https://doi.org/10.3168/jds.S0022-0302(90)78877-7
  16. Ha, J. K. and Lindsay, R. C. 1991. Contributions of cow, sheep, and goat milks to characterizing branched-chain fatty acid and phenolic flavors in varietal cheeses. J. Dairy Sci. 74:3267-3274 https://doi.org/10.3168/jds.S0022-0302(91)78512-3
  17. Ha, J. K. and Lindsay, R. C. 1993. Release of volatile branched-chain and other fatty acids from ruminant milk fats by various lipases. J. Dairy Sci. 76:677-690 https://doi.org/10.3168/jds.S0022-0302(93)77391-9
  18. ISO-IDF. 2002. Milk fat-Preparation of fatty acid methyl esters. International Standard ISO 15884- IDF 182:2002. International Dairy Federation, Brussels, Belgium
  19. Lamberet, G., Delacroix-Buchet, A. and Degas, C. 2001. Intensite de la lipolyse initiale des laits de chevre et perception de l'arome "chevre" dans les fromages. Pages 130-139 in Proc. Technical Symp. 7th Int. Conf. Goats: Recent Advances on Goat Milk Quality, Raw Material for Cheesemaking. (ITPLC Ed.). Poitiers, France, 20 May 2000
  20. Marshall, R. T. 1993. Standard methods for the examination of dairy products. Am. Public Health Association
  21. Matak, K. E., Sumner, S. S., Duncan, S. E., Hovingh, E., Worobo, R. W. and Hackney, C. R. 2007. Effects of ultraviolet irradiation on chemical and sensory properties of goat milk. J. Dairy Sci. 90:3178-3186 https://doi.org/10.3168/jds.2006-642
  22. Shipe, W. F., Senyk, G. F. and Fountain, K. B. 1980. Modified copper soap solvent extraction method for measuring free fatty acids in milk. J. Dairy Sci. 63:193-198 https://doi.org/10.3168/jds.S0022-0302(80)82913-4
  23. 국립수의과학검역원. 2007. 축산물의 가공기준 및 성분규격. 국립수의과학검역원 고시 제2007-1호

Cited by

  1. Characteristics of Cow Milk and Goat Milk Yogurts Fermented by Streptococcus thermophilus LFG Isolated from Kefir vol.33, pp.6, 2013, https://doi.org/10.5851/kosfa.2013.33.6.787