Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Effect of Dietary Lactobacillus on Growth Performance, Intestinal Microflora, Development of Ileal Villi, and Intestinal Mucosa in Broiler Chickens

유산균의 급여가 육계 생산성, 장내 미생물 균총, 회장 융모 발달 및 장점막에 미치는 영향

  • Kim, Sang-Ho (Poultry Science Division, National Institute of Animal Science, R.D.A, Korea) ;
  • Kim, Dong-Wook (Poultry Science Division, National Institute of Animal Science, R.D.A, Korea) ;
  • Park, Su-Young (Nonghyup Feed Inc., Korea) ;
  • Kim, Ji-Hyuk (Poultry Science Division, National Institute of Animal Science, R.D.A, Korea) ;
  • Kang, Geun-Ho (Poultry Science Division, National Institute of Animal Science, R.D.A, Korea) ;
  • Kang, Hwan-Ku (Poultry Science Division, National Institute of Animal Science, R.D.A, Korea) ;
  • Yu, Dong-Jo (Poultry Science Division, National Institute of Animal Science, R.D.A, Korea) ;
  • Na, Jae-Cheon (Poultry Science Division, National Institute of Animal Science, R.D.A, Korea) ;
  • Lee, Sang-Jin (Poultry Science Division, National Institute of Animal Science, R.D.A, Korea)
  • 김상호 (농촌진흥청 축산과학원 가금과) ;
  • 김동욱 (농촌진흥청 축산과학원 가금과) ;
  • 박수영 (농협사료) ;
  • 김지혁 (농촌진흥청 축산과학원 가금과) ;
  • 강근호 (농촌진흥청 축산과학원 가금과) ;
  • 강환구 (농촌진흥청 축산과학원 가금과) ;
  • 유동조 (농촌진흥청 축산과학원 가금과) ;
  • 나재천 (농촌진흥청 축산과학원 가금과) ;
  • 이상진 (농촌진흥청 축산과학원 가금과)
  • Published : 2008.10.01
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Abstract

This experiment was conducted to investigate the effects of dietary supplementation of various Lactobacilli strains on growth performance, intestinal microflora, ileal villi development and ileal mucosal surface of broiler chickens. Six hundred 1-d-old male chicks(Avian) were randomly divided into five groups of four replicates, each replicates containing 30 birds and fed corn-soybean meal diets containing Latobaillus were isolated from cecum of chichens at 107cfu/g diet for five weeks. The treatments were control(antibiotics-free diet), Lactobacillus crispatus avibro1(LCB), Lactobacillus reuteri avibro2(LRB), Lactobacillus crispatus avihen1(LCH) or Lactobacillus vaginalis avihen2(LVH). The body weight gain in groups fed Lactobacillus significantly improved as compared to those of control(P<0.05). Feed intake was not statistically different among the groups. The number of Lactobacillus in ileum and cecum of chicks fed various Lactobacillus tended to be increased or significantly increased as compared to those of the control(P<0.05), but there was difference by age of chicks and species of Lactobacillus. The number of yeast was significantly increased in cecum and ileum at three weeks old chicks fed Lactobacilli compared with the control(P<0.05). The anaerobes’ number of ileum and cecum tended to increase or significantly increased in Lactobacillus treatments compared with the control(P<0.05). The ileal villi length extended greatly at three weeks of age in groups fed Lactobacillus compared with the control(P<0.05). The length of ileal villi in chicks fed Lactobacillus was continuously increased up to five weeks of age, but did not increased in the control(P<0.05). Lactobacillus was found on ileal mucosal surface. And ileal mucosal surface was maintained better with Latobacillus feeding. These results suggest the possibility that cecal Lactobacillus of chickens could be used as probiotics by improving the growth performance and promoting development of ileal villi in broiler chicks.

본 연구는 유산균의 급여가 육계 생산성, 장내미생물 균총, 융모 발달에 미치는 영향을 구명하고 유산균의 장내 거주 상태와 점막 조직을 관찰하고자 수행되었다. 1일령 육계 수평아리(Avian) 600수 육계를 공시하여 5처리 4반복 반복당 30수씩 완전 임의배치 하고, 육계 및 산란계의 맹장에서 유래한 Lactobacillus crispatus avibro1(LCB), Lactobacillus reuteri avibro2 (LRB), Lactobacillus crispatus avihen1(LCH), Lactobacillus vaginalis avihen2 (LVH)을 사료 g당 107cfu 수준으로 급여하였다. 5주 종료체중 및 증체량은 대조구인 유산균 무첨가구에 비해 유산균 첨가구에서 유의적으로 증가하였다(P<0.05). 사료 섭취량은 처리구간 차이가 나타나지 않았다. 유산균의 경우 주령 및 유산균의 종류에 따라 차이를 보이기는 했으나 유산균 첨가시 대조구에 비해 유의하게 증가하는 경향을 보였다. Yeast의 경우 회장에서 1주령까지는 장내에 완전히 정착하지 못하는 것으로 생각되며, 맹장 및 3주령 이후의 회장에서는 대부분의 유산균 첨가구에서 yeast의 수가 증가하였다(P<0.05). 회장 및 맹장 내 혐기성 미생물 역시 유산균 급여구에서 유의하게 증가하거나 증가하였다(P<0.05). 광학 현미경적 관찰에서 회장 융모는 3주령까지 급격하게 신장되고 이후 완만한 성장 양상을 보였는데, 3주령 이후 유산균 급여구는 지속적으로 성장하는데 반하여 대조구는 성장이 지연되는 것으로 나타났다(P<0.05). 전자 현미경적 관찰에서 유산균은 소화기관 점막부근에 거주하며 점막조직에 영향을 미치는 것으로 관찰되었고 또, 유산균 급여구가 무첨가에 비하여 점막조직 발달 상태가 양호한 개체가 많았다. 본 연구 결과 육계 및 산란계 유래 유산균의 급여는 육계 생산성을 향상시키고 회장 융모 발달에 긍정적인 영향을 미쳐 생균제로서의 이용 가능성이 있을 것으로 사료된다.

Keywords

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