Detection of porcine circovirus 2, porcine reproductive and respiratory syndrome virus and Mycoplasma hyopneumoniae from swine lungs with lesions by PCR

돼지 폐렴병변에서 PCR을 이용한 써코바이러스 2, 돼지생식기호흡기증후군, 마이코플라즈마 폐렴 감염실태 조사

  • Chu, Keum-Suk (Jeongeup-Branch, Jeonbuk Institute of Livestock & Veterinary Research) ;
  • Kang, Mi-Seon (Jeongeup-Branch, Jeonbuk Institute of Livestock & Veterinary Research) ;
  • Jo, Young-Suk (Jeongeup-Branch, Jeonbuk Institute of Livestock & Veterinary Research) ;
  • Lee, Jeong-Won (Jeongeup-Branch, Jeonbuk Institute of Livestock & Veterinary Research)
  • 추금숙 (전라북도축산위생연구소 정읍지소) ;
  • 강미선 (전라북도축산위생연구소 정읍지소) ;
  • 조영숙 (전라북도축산위생연구소 정읍지소) ;
  • 이정원 (전라북도축산위생연구소 정읍지소)
  • Published : 2008.03.30

Abstract

Today swine respiratory disease is one of the most important diseases because of its economic losses and severe infection nationwide, and swine society as well as veterinary service are trying to prevent the diseases in Korea. This study would like to obtain some information useful for the control of the diseases. A total of 174 lung specimens with lesion consisted of 3 sorts; 60 were collected from nursey pigs requested for diagnostic service from March of 2006 to October of 2007, 58 finishing pigs and 56 sows were selected from slaughterhouse from September to November 2007. In the detection test of pathogens by PCR, porcine circovirus 2 (PCV2), porcine reproductive and respiratory syndrome virus (PRRSV) and Mycoplasma hyopneumoniae were positive in 95.4%, 31.6%, and 20.1%, respectively. Double infection rate with PCV2 and PRRS was 30.4%, PCV2 and M hyopneumoniae was 19.5%, triple infection with PCV2, PRRS and M hyopneumoniae was 5.7%, respectively.

Keywords

References

  1. Joaquim S, Gordon MA, Mariano D, et al. 2006. porcine circovirus disease. In : Diseases of swine. 9 eds. Iowa State University Press, Ames, Iowa: 299-307
  2. Allan GM, Ellis JA. 2000. Porcine circoviruses : a review. J Vet Diagn Invest 12(1) : 3-14 https://doi.org/10.1177/104063870001200102
  3. Larochelle R, Magar R, D'Allaire S. 2002. Genetic characterization and phylogenetic analysis of porcine circovirus type 2 (PCV2) strains from cases presenting various clinical conditions. Virus Res 90 (1) : 101-112 https://doi.org/10.1016/S0168-1702(02)00141-7
  4. Opriessnig T, Thacker EL, Yu S, et al. 2004. Experimental reproduction of postweaning multisystemic wasting syndrome in pigs by dual infection with Mycoplasma hyopneumoniae and porcine circovirus type 2. Vet Pathol 41(6) : 624-640 https://doi.org/10.1354/vp.41-6-624
  5. Opriessing T, Mckeown NE, Harmon KL, et al. 2006. Porcine circovirus type 2 infection decreases the efficacy of a modified live porcine reproductive and respiratory syndrome virus vaccine. Clin Vaccine Immunol 13(8) : 923-929 https://doi.org/10.1128/CVI.00074-06
  6. Rovira A, Balasch M, Segales J, et al. 2002. Experimental inoculation of conventional pigs with porcine reproductive and respiratory syndrome virus and porcine circovirus 2. J Virol 76 (7) : 3232-3239 https://doi.org/10.1128/JVI.76.7.3232-3239.2002
  7. Haruna J, Hanna P, Hurnik D, et al. 2006. The role of immunostimulation in the development of post- weaning multi-systemic wasting syndrome in pigs under field conditions. Can J Vet Res 70(4) : 269-276
  8. Ellis J, Hassard L, Clark E, et al. 1998. Isolation of circovirus from lesions of pigs with postweaning multisystemic wasting syndrome. Can Vet J 39 (1) : 44-51
  9. Choi C, Chae C. 1999. In-situ hybridization for the detection of porcine circovirus in pigs with postweaning multisystemic wasting syndrome. J Comp Pathol 121 (3) : 265-270 https://doi.org/10.1053/jcpa.1999.0315
  10. Lyoo YS, Kim JH, Park CK. 1999. Identification of porcine circoviruses with genetic variation from lymph nodes collected in pigs with PMWS. Korea J Vet Res 39 (2) : 353-358
  11. Harding J, Clark EG. Eliis JA. 1999. Recognizing and diagnosing postwean ing multisystemic wasting syndrome (PMWS). Swine Health Prod 5 : 201-203
  12. http://www.octagon-services.co.uk/ articles/PMWSvaccine.htm
  13. Mckeown NE, Opriessing T, Thomas PK, et al. 2005. Effects of porcine circovirus type 2 (PCV2) maternal antibodies on experimental infection of piglets with PCV2. Clin Diagn Lab Immunol 12(11) : 1347-1351
  14. Gagnon CA, Tremblay D, Tijssen P, et al. 2007. The emergence of porcine circovirus 2b genotype (PCV-2b) in swine in Canada. Can Vet J 48 (8) : 811-819
  15. Krakowka S, Ellis JA, McNeilly F, et al. 2001. Activation of the immune system is the pivotal event in the production of wasting disease in pigs infected with porcine circovirus-2 (PCV-2). Vet Pathol 38(1) : 31-42 https://doi.org/10.1354/vp.38-1-31
  16. McNeilly F, Kennedy S, Moffett D, et al. 1999. A comparison of in situ hybridization and immunohistochemistry for the detection of a new porcine circovirus in formalin-fixed tissues from pigs with postweaning multisystemic wasting syndrome (PMWS). J Viral Methods 80(2) : 123-128 https://doi.org/10.1016/S0166-0934(99)00043-9
  17. Lyoo KS, Park YH, Park BK. 2001. Prevalence of porcine reproductive and respiratory syndrome virus, porcine circovirus type 2 and porcine parvovirus from fetus and pigs with respiratory problems in Korea. J Vet Sci 2(3) : 201-207
  18. 박최규, 김현수. 2004. 이유자돈 전신소 모성증후군 이환 자돈에서의 바이러스성 원인체 검색 및 porcine circovirus 2 분리 동정. 대한수의학회지 44(4) : 561-569
  19. 김영환, 조광현, 정영석 등. 2005. 경북지방 돼지의 유산태아에서 PCV2 감염률 조사. 한가위지 28(3) : 267-273
  20. 김재훈, 노인순, 손현주 등. 2003. 국내 이유자돈의 써코바이러스 감염에 의한 이유휴전선소모성 증후군. 대한수의학회지 43 (3) : 463-469
  21. 김영환, 조광현, 김성국 등. 2004. 경북지방 돼지에서 이유후전신소모성증후군 및 porcine circovirus type2의 감염 양상. 한가위지 27 (2) : 139-146
  22. 노인순, 이경우, 김재훈 등. 2007. 국내 분리 porcine circovirus 2의 이유자돈에 대한 병원성 시험연구. 대한수의학회지 47 (2) : 175-185