Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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UV Protection and Whitening Effects of Collagen Isolated from Outer Layer of the Squid Todarodes pacificus

동해 오징어 (Todarodes pacificus) 껍질 유래 콜라겐의 UV 보호능 및 미백효과

  • Kwon, Min-Chul (College of Bioscience and Biotechnology, Kangwon National University, Research Institute of Bioscience and Biotechnology, Kangwon National University) ;
  • Qadir, Syed Abdul (College of Bioscience and Biotechnology, Kangwon National University, Research Institute of Bioscience and Biotechnology, Kangwon National University) ;
  • Kim, Hyo-Sung (College of Bioscience and Biotechnology, Kangwon National University, Research Institute of Bioscience and Biotechnology, Kangwon National University) ;
  • Ahn, Ju-Hee (College of Bioscience and Biotechnology, Kangwon National University, Research Institute of Bioscience and Biotechnology, Kangwon National University) ;
  • Cho, Nam-Hyun (Hudem Food Company) ;
  • Lee, Hyeon-Yong (College of Bioscience and Biotechnology, Kangwon National University, Research Institute of Bioscience and Biotechnology, Kangwon National University)
  • 권민철 (강원대학교 BT 특성화학부대학, 강원대학교 생명공학연구소) ;
  • ;
  • 김효성 (강원대학교 BT 특성화학부대학, 강원대학교 생명공학연구소) ;
  • 안주희 (강원대학교 BT 특성화학부대학, 강원대학교 생명공학연구소) ;
  • 조남현 (휴뎀식품) ;
  • 이현용 (강원대학교 BT 특성화학부대학, 강원대학교 생명공학연구소)
  • Published : 2008.02.29
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Abstract

To develop a whitening agent, cytotoxicity of the soluble collagen isolated from Todarodes pacificus (CIT) was evaluated. CIT tested for cytotoxicity on human dermal fibroblast (CCD-986sk) was exhibited very low cytotoxicity. Because tyrosinase is the key enzyme for melanin biosynthesis, the use of various tyrosinase inhibitors is a common practice for whitening purpose in cosmetics. Tyrosinase inhibitory activity and melanin production assay were measured to confirm the whitening effect. The inhibitory effect of MMP-1 in UV-irradiated human dermal fibroblast was also performed. CIT showed strong inhibition potency on tyrosinase by 51.5% at 0.2 mg/mL which increased the inhibition by increasing the concentration of CIT, and showed 69.1% inhibition at 1.0 mg/mL. CIT showed strong inhibition effect on melanin production with 82% at 1.0 mg/mL. The CIT also reduced about 76% expression of MMP-1 in UV-irradiated CCD-986sk cell at 1.0 mg/mL. From the preliminary observations, we suggest that the collagen isolated from CIT could be a potential source of natural skin-whitening and anti-aging agents for the photo-damaged skin.

Keywords

References

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