KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Antimutagenic Effect and Cytotoxicity to Human Cancer Cell Lines of Colored Potato Extracts

유색감자 추출물의 항돌연변이 및 항암 활성

  • Park, Young-Eun (National Institute of Highland Agriculture, RDA) ;
  • Jeong, Jin-Cheol (National Institute of Highland Agriculture, RDA) ;
  • Cho, Huyn-Mook (National Institute of Highland Agriculture, RDA) ;
  • Hwang, Young-Sun (Dept. of Herbal Medicine Resource, Kangwon National University) ;
  • Lee, Hyeon-Jin (Dept. of Herbal Medicine Resource, Kangwon National University) ;
  • Choi, Su-San-Na (Dept. of Herbal Medicine Resource, Kangwon National University) ;
  • Lee, Su-Jin (Dept. of Herbal Medicine Resource, Kangwon National University) ;
  • Park, Eun-Sun (Dept. of Herbal Medicine Resource, Kangwon National University) ;
  • Ko, Eun-A (Dept. of Herbal Medicine Resource, Kangwon National University) ;
  • Kim, Nan-Sol (Dept. of Herbal Medicine Resource, Kangwon National University) ;
  • Lim, Jung-Dae (Dept. of Herbal Medicine Resource, Kangwon National University) ;
  • Choung, Myoung-Gun (Dept. of Herbal Medicine Resource, Kangwon National University)
  • 박영은 (농촌진흥청 고령지농업연구소) ;
  • 정진철 (농촌진흥청 고령지농업연구소) ;
  • 조현묵 (농촌진흥청 고령지농업연구소) ;
  • 황영선 (강원대학교 생약자원개발학과) ;
  • 이현진 (강원대학교 생약자원개발학과) ;
  • 최수산나 (강원대학교 생약자원개발학과) ;
  • 이수진 (강원대학교 생약자원개발학과) ;
  • 박은선 (강원대학교 생약자원개발학과) ;
  • 고은아 (강원대학교 생약자원개발학과) ;
  • 김난솔 (강원대학교 생약자원개발학과) ;
  • 임정대 (강원대학교 생약자원개발학과) ;
  • 정명근 (강원대학교 생약자원개발학과)
  • Published : 2008.03.31
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Abstract

Colored potatoes are an excellent source of dietary polyphenols including anthocyanins. Generally, anthocyanins from fruits and vegetables exhibit anti-carcinogenesis and anti-cancer properties in vitro test. This experiment was conducted to know the effects of colored potato extracts contained anthocyanins on antimutagenic activity and anticancer activity to six human cancer cell lines containing LNCaP (androgen-dependent) prostate cancer cells. Extracts of three colored potatoes ('Hongyoung', 'Jayoung' and 'Jasim') and the white potato ('Superior') cultivars were used in this study. The extracts of three colored potatoes inhibited the mutagenicities induced by direct mutagen such as 4-nitro-quinoline-1-oxide (4-NQO) and another indirect mutagens of bezo(a)pyrene (BaP). Also, the extracts of 'Hoyoung' and 'Jayoung' showed higher antimutagenic activity than 'Jasim' and 'Superior' against to direct or indirect mutagen on both strains of TA98 and TA100. The activity of growth-inhibitory of extract of four potato cultivars were screened by SRB (sulphorhodamine B) method on diverse human cancer cells representing different types of cancers. Among the extract of four potato cultivars, the extract of 'Jasim' showed moderate inhibition on proliferation of LNCaP, ACHN and MOLT-4F cells and did not inhibit the proliferation of other cancer cells. On the other hand, extract of 'Superior' did not inhibit the proliferation of any tested cancer cell lines. However, the extracts of 'Hongyoung and Jayoung' inhibited the proliferation of cancer cells with $GI_{50}$ values ranging from 2.5 to $30\;{\mu}g/mL$. On the basis of the $GI_{50}$ values, it is clear that LNCaP cells were more sensitive to extracts of colored potato cultivars than other cancer cells. The extract of 'Jayoung' at $30\;{\mu}g/mL$ were more active and inhibited cell proliferation, and induced apoptosis in LNCaP cells. This result revealed that the extracts of colored potatoes are expected to be good candidate for development into source of antimutagenic and anticancer agent.

본 연구는 괴경 내부에 적색 및 보라색의 안토시아닌 색소를 함유한 유색감자의 추출물을 대상으로 S. typhimurium TA98과 TA100 균주의 돌연변이 유발여부를 확인하였고, 직접돌연변이원인 4-nitroquinoline-1-oxide(4-NQO)와 간접 돌연변이원인 bozo(a)pyrene(BaP)에 의해 유발될 수 있는 돌연변이에 대한 항돌연변이 활성과 6종의 인간 암세포주(전립선암세포주: LNCaP, 결장암 세포주: HCT-15와 SW-620, 위암 세포주: ACHN, 폐암 세포주: A549, 백혈병 세포주: MOLT-4F)를 대상으로 SRB 방법을 이용하여 항암활성을 비교하였다. 그 결과 유색감자 중 괴경 내부에 보라색의 안토시아닌을 다량 함유한 자영 품종이 다른 품종에 비해 높은 수준의 항돌연변이활성 및 항암활성을 나타내었으며, 특히 자영 품종의 추출물은 다른 세포주 보다 전립선암 세포주에 대한 항암활성이 특이적으로 우수한 양상을 확인하였다. 자영 품종의 추출물은 $5\;{\mu}g/mL$ 이상의 농도에서 암세포의 증식을 억제할 뿐 아니라, 전립선암 세포주 LNCaP와 PC-3에 대해 세포사멸을 유발하는 결과를 Cell Death Detection ELISA와 TUNEL Assay로 확인하였고, 세포사멸과 연관된 유전자의 발현분석을 western blot으로 확인하였다. 이상의 결과에서 괴경 내부에 안토시아닌을 함유한 유색감자는 괴경 내부의 색상이 백색인 일반감자에 비해 강한 항돌연변이활성 및 항암활성을 나타내므로 유색감자는 기능성이 증대된 식용감자로서의 이용가치가 충분하며, 유색감자를 이용한 기능성식품 소재개발 및 의료산업의 신규소재화가 가능하리라 판단된다.

Keywords

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