Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
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The Effects of Vitamin D Supplementation on Bone Mineral Density and Bone Mineral Content in Ovariectomized Rats

난소절제 쥐에서 비타민 D 첨가식이가 골밀도와 골무기질 함량에 미치는 영향

  • Choi, Mi-Ja (Department of Food and Nutrition, Keimyung University) ;
  • Kim, Sang-Mi (Department of Food and Nutrition, Keimyung University)
  • 최미자 (계명대학교 식품영양학과) ;
  • 김상미 (계명대학교 식품영양학과)
  • Published : 2008.03.31
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Abstract

The effects of vitamin $D_{3}$ supplementation on bone mineral density and bone mineral content in ovariectmized rats were investigated. Forty female Sprague-Dawley rats (body weight 180 g) were divided into two group, ovariectomy and Sham groups, which were each randomly divided into two subgroups that were fed casein and casein supplemented with vitamin $D_{3}$ diets. Bone mineral density (BMD) and bone mineral content (BMC) were measured using PIXlmus (GE Lunar Co, Wisconsin, USA) in spine and femur on 6 weeks after feeding. Osteocalcin and urinary DPD crosslinks value were measured as markers of bone formation and resorption. The body weight gain and food efficiency ratio (FER) were higher in OVX groups than in Sham groups regardless of diets. Serum Ca concentration and urinary Ca excretion were higher in vitamin $D_{3}$ supplemented group than in casein group in Sham and OVX. Crosslinks values were higher in OVX groups than in Sham groups. Spine BMD and femur BMD of ovariectomy group were significantly lower than Sham groups, however vitamin $D_{3}$ supplemented groups were significantly higher than control groups in Sham and OVX. In conclusion, dietary vitamin $D_{3}$ supplementation on ovariectimized rats were significantly increased bone mineral density and bone mineral content in spine and femur.

Keywords

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