Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effects of Aqueous Extract from Aconitum Koreanum on the Expression of Tyrosinase-related Proteins by Ultraviolet B Irradiation in Guinea Pig Skin

백부자의 추출물이 자외선 B조사에 의한 기니피그 피부의 tyrosinase-related proteins발현에 미치는 영향

  • Lee, Sang-Bok (Department of Oriental Medicine, College of Oriental Medicne, Dong-Eui University) ;
  • Park, Dong-Il (Department of Oriental Medicine, College of Oriental Medicne, Dong-Eui University) ;
  • Kim, Hoon (Department of Oriental Medicine, College of Oriental Medicne, Dong-Eui University) ;
  • Gil, Young-Gi (Department of Anatomy, Medical School, Kosin University) ;
  • Choi, Byung-Tae (Department of Anatomy, School of Oriental Medicine, Pusan National University)
  • 이상복 (동의대학교 한의과대학 한의학과) ;
  • 박동일 (동의대학교 한의과대학 한의학과) ;
  • 김훈 (동의대학교 한의과대학 한의학과) ;
  • 길영기 (고신대학교 의과대학 해부학교실) ;
  • 최병태 (부산대학교 한의학전문대학원 해부학교실)
  • Published : 2008.04.25
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Abstract

To investigate whether aqueous extract from Aconitum koreanum (AEAK) effects in the process of melanin synthesis, the expression of tyrosinase-related proteins (TRPs) by immunohistochemical methods were performed in ultraviolet B (UVB) irradiated skin of guinea pig. The irradiation of UVB (60 mJ/day) was performed for 3 days and treated with AEAK for 15 days. About the color evaluation, the visual scores of UV B irradiated guinea pig with AEAK treatment were slightly lower than those in the UV B alone irradiated ones. At day 15 after UVB exposure, immunohistochemical analysis for TRPs expression were performed. The intensive expression of tyrosinase was mainly observed over epidermis with skin appendage and in the cells of dermis. Slight increase of these reaction was induced in response to UVB in the spinous and granular layer of epidermis, but similar expression in the AEAK treated guinea pig as normal one. The TRP-1 and TRP-2 expression were not detected in the skin of normal guinea pig. But intensive expression for TRP-1 and TRP-2, especially TRP-2, induced by UV B irradiation in the cells of dermis. These expressions were decreased in the AEAK treated guniea pig. Collectively, these results suggest that AEAK has a potential to inhibit synthesis through regulation of TRPs expression in the skin of guinea pig, but better understanding the function of AEAK, more research should be done in the effects of AEAK on the function of TRPs in melanogesis.

Keywords

References

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