Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Culture Conditions for Glutathione Maximum Production by Saccharomyces cerevisiae FF-8 in Bioreactor

Saccharomyces cerevisiae FF-8를 이용한 글루타티온의 대량 생산을 위한 배양 조건

  • Cha, Jae-Young (Alcoholic Beverage Research Institute, Daesun Distilling Co., Ltd.) ;
  • Park, Sang-Hyun (Miabu fermentation science reaserch institute, Miabu Co., Ltd.) ;
  • Heo, Jin-Sun (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University) ;
  • Park, Bo-Kyung (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University) ;
  • Lee, Jin-Woo (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University) ;
  • Kim, Jung-Wook (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University) ;
  • Cho, Young-Su (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University)
  • 차재영 (대선주조(주) 부설 기술연구소) ;
  • 박상현 ((주)미애부 발효과학연구소) ;
  • 허진선 (동아대학교 생명공학과) ;
  • 박보경 (동아대학교 생명공학과) ;
  • 이진우 (동아대학교 생명공학과) ;
  • 김정욱 (동아대학교 생명공학과) ;
  • 조영수 (동아대학교 생명공학과)
  • Published : 2008.05.30
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Abstract

As glutathione is now widely used in medicine, functional health food and cosmetic industry, the commercial demand for glutathione has been expending. We have previously reported that Saccharomyces cerevisiae FF-8, an intracellular glutathione producing yeast strain, was isolated from Korean traditional rice wine. Glutathione production by FF-8 strain was 90 mg/l from basal YM medium in flask-scale fermentation, but that in optimal YM medium increased 2.27-fold to 204 mg/l. As large scale fermentation is generally applied to achieve more efficient production of bioactive components, this study was also tried to optimization of fermentation parameters to enhance the glutathione production using S. cerevisiae FF-8 in bioreactor process. The highest cell growth and glutathione concentrations of S. cerevisiae FF-8 in 7 l fermenter were observed to be the culture conditions containing culture temperature $30^{\circ}C$, adjusted initial pH 6.0, agitation speed 200 rpm, and aeration rate 2.0 vvm. The maximum glutathione production by FF-8 was cultured under the inner pressure $0.8 kgf/cm^2$ in a 100 l bioreactor and it was 255.8 mg/l. The investigation of significant parameters for improving the glutathione production could be very useful in optimizing processes.

글루타티온은 의약품, 건강기능식품, 화장품 등에 널리 사용되고 있어서 오늘날 상업적인 수요가 증가하고 있다. 한국전통 발효주로부터 글루타티온-고함유 효모인 S. cerevisiae FF-8 균주를 분리한 바 있으며, FF-8 균주에서 글루타티온 생산량은 YM 기본배지를 이용한 플라스크 배양에서 90 mg/l이었으나, YM 최적배지에서는 2.27배 증가한 204 mg/l 이었다. 대량배양 발효방법은 일반적으로 효모에서 생리활성 물질을 더욱 효과적으로 생산하기 위하여 대량배양기를 이용한 S. cerevisiae FF-8에서 글루타티온 생산량을 증대시킬 수 있는 최적의 발효조건을 탐색하였다. 7 l 발효기에서 FF-8 균주에 의한 세포생육과 글루타티온 최대 생산량을 위한 발효조건은 교반 속도 200 rpm, 배양 온도 $30^{\circ}C$, 초기 조절 pH 6.0, 통기조건 2.0 vvm이었다. 또한 100 l 대량배양기에서 S. cerevisiae FF-8에 의한 글루타티온 대량생산 조건은 내부압력 0.8 $kgf/cm^2$ 이었고, 이때 글루타티온 생산량은 225.8 mg/l이었다. 글루타티온 생산량을 증대시키기 위한 중요한 요소의 검토는 최적의 프로세스 확립에 매우 유용하다.

Keywords

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