Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Acanthopanax senticosus Extract Acts as an Important Regulator for Vascular Functions.

혈관 생리 활성에 미치는 가시오가피 추출물의 효능

  • Choi, Seung-Min (Department of Molecular Biology & Institute of Nanosensor and Biotechnology, BK21 Graduate Program for RNA Biology, Dankook University) ;
  • Park, Jong-Bong (Department of Molecular Biology & Institute of Nanosensor and Biotechnology, BK21 Graduate Program for RNA Biology, Dankook University) ;
  • Kim, Jong-Min (Department of Molecular Biology & Institute of Nanosensor and Biotechnology, BK21 Graduate Program for RNA Biology, Dankook University) ;
  • In, Kyung-Min (Department of Molecular Biology & Institute of Nanosensor and Biotechnology, BK21 Graduate Program for RNA Biology, Dankook University) ;
  • Park, Heon-Yong (Department of Molecular Biology & Institute of Nanosensor and Biotechnology, BK21 Graduate Program for RNA Biology, Dankook University)
  • 최승민 (단국대학교 분자생물학과, BK21 RNA 전문인력양성팀, 나노센서바이오텍연구소) ;
  • 박종봉 (단국대학교 분자생물학과, BK21 RNA 전문인력양성팀, 나노센서바이오텍연구소) ;
  • 김종민 (단국대학교 분자생물학과, BK21 RNA 전문인력양성팀, 나노센서바이오텍연구소) ;
  • 인경민 (단국대학교 분자생물학과, BK21 RNA 전문인력양성팀, 나노센서바이오텍연구소) ;
  • 박헌용 (단국대학교 분자생물학과, BK21 RNA 전문인력양성팀, 나노센서바이오텍연구소)
  • Published : 2008.05.30
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Abstract

Crude extracts of Acanthopanax senticosus (A. senticosus) have been used as galenicals. In this study we carried out a series of experiments to examine cardiovascular activities of crude extracts from A. senticosus. Whereas the extracts had no effect on the apoptosis of bovine aortic endothelial cells (BAECs), the extracts appeared to induce endothelial cell proliferation. In addition, endothelial cell migration was shown to be enhanced by the crude extracts, indicating that A. senticosus extracts likely act as an angiogenic factor. We also examined effects of A. senticosus extracts on nitric oxide (NO) production, leukocytic adhesion to endothelium, and homotypic aggregation of leukocytes. Both the NO production and the leukocytic adhesion to endothelial cells were revealed to be increased by treatment with A. senticosus extracts, but the homotypic aggregation of leukocytes was inhibited. Together, multiple roles of A. senticosus extracts in the cardiovascular system provide an insight on their broad applications as galenicals to treat cardiovascular diseases.

생약 재료로 사용되어 왔던 가시오가피 추출액의 혈관 생리 활성을 평가하기 위하여 혈관 내피세포에서 다양한 실험을 수행하였다. 가시오가피 추출액은 혈관 내피세포 성장을 촉진하면서 세포사멸에는 영향을 미치지 않았다. 또한 혈관 내피세포의 세포 이동이 가시오가피 추출액에 의하여 촉진됨을 알 수 있었다. 이와 같은 연구 결과를 보면, 가시오가피 추출액이 혈관 형성을 유발시키는 작용을 하는 것으로 예측된다. 또한 가시오가피 추출액이 산화질소(NO)의 생산을 증가시키는 기능과 THP-1의 내피세포 부착을 억제하는 기능을 동시에 갖고 있음을 확인하였다. 이러한 결과는 가시오가피가 동맥경화 발생을 억제시키는 약물임을 의미한다. 또한 가시오가피 추출액은 THP-1 세포의 동종세포 응집을 촉발하는 기능을 함으로서 상처 난 부위에서 일어나는 출혈을 억제할 것으로 예측된다. 결론적으로 가시오가피 추출액은 다양한 혈관 생리 활성을 갖는 생약 재료로 평가된다.

Keywords

References

  1. Brekhmann II. and IV Daraymov. 1969. New substance of plant origin which increase nonspecific resistance. Annu. Rev. Pharmacol. 9, 419-430. https://doi.org/10.1146/annurev.pa.09.040169.002223
  2. Broillet, M., O. Randin and J. Chatton. 2001. Photoactivation and calcium sensitivity of the fluorescent NO indicator 4,5-diaminofluorescein (DAF-2): implications for cellular NO imaging. FEBS Lett. 491, 227-232. https://doi.org/10.1016/S0014-5793(01)02206-2
  3. Chang, S. Y., K. S. Ryu, D. H. Lee and Y. K. Seo. 1976. Program the annual convention of the Korean society of pharmacognosy: Studies on the Lignan group from Acanthopanax sessiliflorum. Kor. Soc. Pharmacogn. 7, 255-255.
  4. Charrier, L., Y. Yan, H. T. Nguyen, G. Dalmasso, C. L. Laboisse, A. T. Gewirtz, S. V. Sitaraman and D. Merlin. 2007. ADAM-15/metargidin mediated homotypic aggregation of human T lymphocytes and heterotypic interactions of T lymphocytes with intestinal epithelial cells. J. Biol. Chem. 282, 16948-16958. https://doi.org/10.1074/jbc.M700158200
  5. Cho, K. H., W. S. Jung, S. U. Park, S. K. Moon, C. N. Ko, S. Ku, S. G. Chi and H. Park. 2004. Dio-Orengedokudo works as a cell-proliferating compound in endothelial cells. Can. J. Physiol. Pharmacol. 82, 380-386. https://doi.org/10.1139/y04-036
  6. Dattner, A. M. 2003. From medical herbalism to phytotherapy in dermatology: back to the future. Dermatologic Therapy 16, 106-113. https://doi.org/10.1046/j.1529-8019.2003.01618.x
  7. Davydov, M. and A. D. Krikorian. 2000. Eleutherococcus senticosus (Rupr. & Maxim.) Maxim. (Araliaceae) as an adaptogen: a closer look. J. Ethnophaemacol. 72, 345-393. https://doi.org/10.1016/S0378-8741(00)00181-1
  8. Denes, L., A. Jednakovits, J. Hargitai, Z. Penzes, A. Balla, L. Talosi, P. Krajcsi and P. Csermely. 2007. Pharmacologically activated migration of aortic endothelial cells is mediated through p38 SAPK. Br. J. Pharmacol. 136, 597-603. https://doi.org/10.1038/sj.bjp.0704738
  9. Edgaras, S., K. Egidijus, V. Enrikas and S. Uif. 2003. Role of nitric oxide and other endothelium-derived factors. MEDICINA. 39, 333-341.
  10. Eriksson, E. E., X. Xie, J. Werr, P. Thoren and L. Lindbom. 2001. Direct viewing of atherosclerosis in vivo: plaque invasion by leukocytes is initiated by the endothelial selectins. FASEB J. 15, 1149-1157. https://doi.org/10.1096/fj.00-0537com
  11. Fransen, P., T. Van Assche, P. J. Guns, C. E. Van Hove, G. W. DE Keulenaer, A. G. Herman and H. Bult. 2008. Endothelial function in aorta segments of apolipoprotein E-deficient mice before development of atherosclerotic lesions. Pflugers Arch. 455, 811-818. https://doi.org/10.1007/s00424-007-0337-9
  12. Gewaltig, M. T. and G. Kojda. 2002. Vasoprotection by nitric oxide: mechanisms and therapeutic potential. Cardiovasc. Res. 55, 250-260. https://doi.org/10.1016/S0008-6363(02)00327-9
  13. Heo, I. D., H. J. Seo and J. D. Kim. 2007. Anti-angiogenic and anti-cell adhesion effect of the Camellia japonica flower extract. J. Life Science 17, 1152-1156. https://doi.org/10.5352/JLS.2007.17.8.1152
  14. Iyer, S., D. J. Chaplin, D. S. Rsnthal, A. H. Boulares, L. Li and M. E. Smulson. 1998. Induction of apoptosis in proliferating human endothelial cells by the tumor-specific antiangiogenesis agent combretastatin A-4. Cancer Res. 58, 4510-4514.
  15. Karin, M., T. Lawrence and V. Nizet. 2006. Innate immunity gone awry: linking microbial infections to chronic inflammation and cancer. Cell 124, 823-835. https://doi.org/10.1016/j.cell.2006.02.016
  16. Kim, J. M., K. M. In, S. M. Choi, J. H. Pyee and H. Y. Park. 2006. Resveratrol-induced apoptosis is inhibited by nitric oxide in endothelial. cells. J. Nano Bio. Tech. 3, 89-92.
  17. Kim, C. W. and H. Y. Lee. 1990. Studies on the constituents of seeds of Acanthopanax senticosus for inermis Harms. Kor. J. Pharmacogn. 21, 235-238.
  18. Kim, Y. M. 1977. Pharmaceutical comparison of ginseng with Acanthopanax from the aspect of oriental medicine. Kor. J. Pharmacogn. 8, 131-138.
  19. Kojima, H., N. Nakatsubo, K. Kikuchi, S. Kawahara, Y. Kirino, H. Nagoshi, Y. Hirata and T. Nagano. 1998. Detection and imaging of nitric oxide with novel Fluorescent indicators: diaminofluorescein. Anal. Chem. 70, 2446-2453. https://doi.org/10.1021/ac9801723
  20. Lee, C. U and J. B. Koh. 2006. Effects of cheonggukjang on lipid metabolism in female rats fed cholesterol diet. J. Life Science 16, 932-937. https://doi.org/10.5352/JLS.2006.16.6.932
  21. Lee, K. H., J. O. Nam and W. H. Yoon. 2007. Effects of protein- bound polysacharide isolated from Acanthopananx senticosus in reducing the toxic effects of cisplatin. Kor. Soc. Pharmacogn. 38, 152-156.
  22. Libby, P., P. M. Ridker and A. Maseri. 2002. Inflammation and Atherosclerosis. Circulation 105, 1135-1143. https://doi.org/10.1161/hc0902.104353
  23. Lim, S. Y., J. Y. Leem, C. S. Lee, Y. J. Jang, J. W. Park and S. Yoon. 2007. Antioxidant and cell proliferation effects of Acanthopanax senticosus extract in human osteoblast-like MG-63 cell line. Korean J. Food Sci. Technol. 39, 694-700.
  24. Malek, N. P., H. Sundberg, S. McGrew, K. Nakayama, T. R. Kyriakides, J. M. Roberts and T. R. yriakidis. 2001. A mouse knock-in model exposes sequential proteolytic pathways that regulate p27Kip1 in G1 and S phase. Nature 413, 323-327. https://doi.org/10.1038/35095083
  25. Nakatsubo, N., H. Kojima, K. Kikuchi, H. Nagoshi, Y. Hirata, D. Maeda, Y. Imai, T. Irimura and T. Nagano. 1998. Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: diaminofluoresceins. FEBS Lett. 427, 263-266. https://doi.org/10.1016/S0014-5793(98)00440-2
  26. Park, H., S. G. Park, W. Lee, G. Kim, Y. G. KO and S. Kim. 2002. Monocyte cell adhesion induced by a human aminoacyl-tRNA synthetase-associated factor, p43: identificaton of the related adhesion molecules and signal pathway. J. Leukoc. Bio. 71, 223-230.
  27. Ramos, C. L., Y. Huo, U. Jung, S. Ghosh, D. R. Manka, I. J. Sarembock and K. Ley. 1999. Direct demonstration of P-selectin- and VCAM-1-dependent mononuclear cell rolling in early atherosclerotic lesions of apolipoprotein E-deficient mice. Circ. Res. 84, 1237-1244. https://doi.org/10.1161/01.RES.84.11.1237
  28. Sasaki, A., S. Doi, S. Mizutani and H. Azuma. 2007. Roles of accumulated endogenous nitric oxide synthase inhibitors, enhanced arginase activity, and attenuated nitric oxide synthase activity in endothelial cells for pulmonary hypertension in rats. Am. J. Physiol. Lung. Cell Mol. Physiol. 292, L1480-L1487. https://doi.org/10.1152/ajplung.00360.2006

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