Environmental Analysis Health and Toxicology

The Korean Society of Environmental Health and Toxicology (환경독성보건학회)
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Evaluation of Oxy-radical Scavenging Capacity of Fucoidan

Fucoidan의 Oxy-radical 포획능 평가

  • 김봉희 (충남대학교 약학대학 형질전환 복제돼지 센터) ;
  • 오정민 (충남대학교 약학대학 형질전환 복제돼지 센터) ;
  • 강건욱 (조선대학교 약학대학) ;
  • 곽상훈 (LG 생활건강) ;
  • 윤세영 (LG 생활건강) ;
  • 이철호 (한국생명공학연구원, 천연물의약 연구센터) ;
  • 이현선 (한국생명공학연구원, 천연물의약 연구센터) ;
  • 김상겸 (충남대학교 약학대학 형질전환 복제돼지 센터)
  • Published : 2008.03.31
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Abstract

Algal fucoidan is a marine polysaccharide containing sulfur with a wide variety of biological activities including anti-inflammatory and anti-thrombotic effects. Although antioxidants can inhibit inflammatory signals through inhibiting activator protein-1 and/or nuclear factor-kappaB activation, it is obscure whether fucoidan directly scavenges oxy-radicals or indirectly regulates oxidant production and/or antioxidant defense system. The antioxidant activities of fucoidan against peroxyl radicals, peroxynitrites and hydroxyl radicals were determined by the total oxy-radical scavenging capacity (TOSC) assay. The specific TOSC values of fucoidan against peroxyl radicals, peroxynitrites or hydroxyl radicals were $282{\pm}60$, $43{\pm}1$ or $40{\pm}1\;TOSC/mg/mL$, respectively. These specific TOSC values against peroxyl radicals, peroxynitrites or hydroxyl radicals are 23, 12, or 13% of the specific TOSC values of glutathione, a positive control, respectively. These results suggest that fucoidan has direct oxy-radical scavenging capacity, which may be related with anti-inflammatory effect of fucoidan.

Keywords

References

  1. Berteau O and Mulloy B. Sulfated fucans, fresh perspectives: structures, functions, and biological properties of sulfated fucans and an overview of enzymes active toward this class of polysaccharide, Glycobiology 2003; 13(6): 29R-40R https://doi.org/10.1093/glycob/cwg058
  2. Biesalski HK. Polyphenols and inflammation: basic interactions, Curr Opin Clin Nutr Metab. Care 2007; 10(6): 724-728 https://doi.org/10.1097/MCO.0b013e3282f0cef2
  3. Brown GC. Mechanisms of inflammatory neurodegeneration: iNOS and NADPH oxidase, Biochem Soc Trans 2007; 35(Pt 5): 1119-1121 https://doi.org/10.1042/BST0351119
  4. Lu Y and Cederbaum AI. CYP2E1 and oxidative liver injury by alcohol, Free Radic Biol Med 2008; 44(5): 723-738 https://doi.org/10.1016/j.freeradbiomed.2007.11.004
  5. Pannu R and Singh I. Pharmacological strategies for the regulation of inducible nitric oxide synthase: neurodegenerative versus neuroprotective mechanisms, Neurochem Int 2006; 49(2): 170-182 https://doi.org/10.1016/j.neuint.2006.04.010
  6. Regoli F and Winston GW. Quantification of total oxidant scavenging capacity of antioxidants for peroxynitrite, peroxyl radicals, and hydroxyl radicals, Toxicol Appl Pharmacol 1999; 156: 96-105 https://doi.org/10.1006/taap.1999.8637
  7. Sun Y. Oxidative stress and cardiac repair/remodeling following infarction, Am J Med Sci 2007; 334(3): 197-205 https://doi.org/10.1097/MAJ.0b013e318157388f
  8. Winston GW, Regoli F, Dugas AJ Jr., Fong JH and Blanchard KA. A rapid gas chromatographic assay for determining oxyradical scavenging capacity of antioxidants and biological fluids, Free Radic Biol Med 1998; 24: 480-493 https://doi.org/10.1016/S0891-5849(97)00277-3
  9. Yang JW, Yoon SY, Oh SJ, Kim SK and Kang KW. Bifunctional effects of fucoidan on the expression of inducible nitric oxide synthase, Biochem Biophys Res Commun 2006; 346(1): 345-350 https://doi.org/10.1016/j.bbrc.2006.05.135