Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Inhibitory Effect of Dalbergioidin Isolated from the Trunk of Lespedeza cyrtobotrya on Melanin Biosynthesis

  • Baek, Seung-Hwa (Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Jin-Hee (Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Dong-Hyun (Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Chan-Yong (Department of Microbiology and Biotechnology, Daejeon University) ;
  • Kim, Ji-Young (Division of Bioscience and Biotechnology, Konkuk University) ;
  • Chung, Dae-Kyun (Skin Biotechnology Center, Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University) ;
  • Lee, Choong-Hwan (Division of Bioscience and Biotechnology, Konkuk University)
  • Published : 2008.05.31
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Abstract

Tyrosinase is a key enzyme for melanin biosynthesis, and hyperpigmentation disorders are associated with abnormal accumulation of melanin pigments, which can be reduced by treatment with depigmenting agents. The methanol extract of Lespedeza cyrtobotrya $M_{IQ}$ showed inhibitory activity against mushroom tyrosinase. The active compound was purified from the methanol extract of L. cyrtobotrya, followed by several chromatographic methods, and identified as dalbergioidin (DBG) by spectroscopic methods. The results showed that DBG exhibited tyrosinase inhibitory activity with an $IC_{50}$ of $20\;{\mu}M$. The kinetic analysis of tyrosinase inhibition revealed that DBG acted as a noncompetitive inhibitor. In addition, DBG showed a melanin biosynthesis inhibition zone in the culture plate of Streptomyces bikiniensis that has commonly been used as an indicator organism. Furthermore, $27\;{\mu}M$ DBG decreased more than 50% of melanin contents on the pigmentation using the immortalized mouse melanocyte, melan-a cell.

Keywords

References

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