Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Inhibitory Effects of Saururus Chinensis Extracts on Osteoclast Differentiation

  • Shim, Ki-Shuk (Laboratory of Chemical Genomics) ;
  • Kim, Soon-Nam (Laboratory of Chemical Genomics. Department of Biology, Chungnam National University) ;
  • Kim, Myung-Hee (Laboratory of Chemical Genomics, Department of Biochemistry, Chungnam National University) ;
  • Kim, Young-Sup (Laboratory of Phytochemistry Research, Korea Research Institute of Chemical Technology) ;
  • Ryu, Shi-Yong (Laboratory of Phytochemistry Research, Korea Research Institute of Chemical Technology) ;
  • Min, Yong-Ki (Laboratory of Chemical Genomics) ;
  • Kim, Seong-Hwan (Laboratory of Chemical Genomics)
  • Published : 2008.06.30
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Abstract

Saururus chinensis is a commonly used folk herb for the treatment of edema and liver diseases in Korea. To study the biological activity of Saururus chinensis in bone metabolism, we evaluated the effect of its extracts on osteoclast differentiation in vitro using primary mouse bone marrow-derived macrophages. Methanol extract (ME) from dried roots of Saururus chinensis was partitioned into methylene chloride (MF), ethyl acetate (EF), n-butanol (BF) and water fractions (WF). Tartrate-resistance acid phosphatase (TRAP) activity assay and western blot analysis were performed to determine the effect on osteoclast differentiation and mitogen-activated protein (MAP) kinases activation. ME, MF and EF dramatically inhibited receptor activator of ${NF-kB}$ ligand (RANKL)-induced formation of multinucleated osteoclasts and activation of MAP kinases. This study firstly demonstrated that ME, MF and EF of Saururus chinensis have the potential to inhibit the osteoclast differentiation, which results from the inhibition of MAP kinases activations in part.

Keywords

References

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