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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Inhibition effects of extracts and its solvent fractions isolated from Limonium tetragonum on growth of human cancer cells

염생식물 갯질경 [Limonium tetragonum]의 추출물이 암세포성장에 미치는 효과

  • Kong, Chang-Suk (Research Institute of Marine Science and Technology, Korea Maritime University) ;
  • Um, Young-Ran (Division of Marine Environment and Bioscience, Korea Maritime University) ;
  • Lee, Jung-Im (Division of Marine Environment and Bioscience, Korea Maritime University) ;
  • Kim, You-Ah (Division of Marine Environment and Bioscience, Korea Maritime University) ;
  • Lee, Jeom-Sook (Department of Biology, Kusan National University) ;
  • Seo, Young-Wan (Division of Marine Environment and Bioscience, Korea Maritime University)
  • 공창숙 (한국해양대학교 해양과학기술연구소) ;
  • 엄영란 (한국해양대학교 해양환경생명과학부) ;
  • 이정임 (한국해양대학교 해양환경생명과학부) ;
  • 김유아 (한국해양대학교 해양환경생명과학부) ;
  • 이점숙 (군산대학교 생물학과) ;
  • 서영완 (한국해양대학교 해양환경생명과학부)
  • Published : 2008.04.29
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Abstract

In this study, crude extracts of the halophyte Limonium tetragonum and their solvent fractions were evaluated on anticancer activity in AGS and HT-29 human cancer cells using MTT assay. Each of the crude extracts (MeOH and $CH_2Cl_2$) of Limonium tetragonum showed a significant inhibitory effect on the growth of human cancer cells. The combined crude extracts of MeOH and $CH_2Cl_2$ were partitioned between $CH_2Cl_2$ and water. The organic layer was further partitioned between 85% aq. MeOH and n-hexane, and then the aqueous layer was fractionated with n-BuOH and $H_2O$, successively. Growth inhibition effects of crude extracts and their solvent fractions from Limonium tetragonum increased in a dose-dependent manner. Among them, 85% aq. MeOH, n-hexane and n-BuOH fractions revealed very good inhibition effects on the growth of human cancer cells. These results suggest that we can isolate active compounds from Limonium tetragonum to show much more strong anticancer activity.

본 연구에서는 염생식물의 한 종류인 갯질경을 이용하여 생리활성물질에 의한 항발암성물질의 생성 및 생체방어물질로서의 유용성을 검토하기 위하여 갯질경의 추출물 및 용매별 분획물에서 AGS와 HT-29 인체암세포에 대한 증식억제효과를 검토하였다. 갯질경의 용매 추출물은 시료 처리 농도 증가에 따라 인체 위암 및 결장암세포의 생존율을 농도 의존적으로 감소시키는 경향을 보였다. 시료의 처리농도 $200\;{\mu}g/ml$에서 methanol과 methylene chloride 추출물은 인체 위암세포에 대하여 각각 66%와 71%의 암세포 증식억제율을, 인체 결장암세포에 대하여서는 각각 60%와 84%의 암세포 증식억제율을 보였다. 이러한 암세포 증식억제효과는 methanol 추출물보다는 methylene chloride 추출물에서 높게 나타났으며, AGS 인체 위암세포에서 보다도 HT-29 인체 결장암세포에서 다소 높은 효과를 나타내었다. 갯질경의 조추출물이 인체 암세포에 대하여 높은 증식억제효과를 나타내었으므로, 이들 추출물의 용매분획물을 이용하여 인체 암세포의 성장에 미치는 영향을 살펴 보았다. 그 결과 85%aq. MeOH 분획층에서 가장 높은 암세포 증식 억제효과를 보였으나, $H_2O$ 분획층에서는 50%이하의 낮은 억제율로 거의 효과를 보이지 않았다. AGS 인체 위암세포의 성장억제효과를 관찰한 결과, $200\;{\mu}g/ml$의 처리 농도에서 85% aq. MeOH, n-hexane 및 n-BuOH의 분획물이 약 80% 이상의 높은 증식 억제효과를 나타내었으며, HT-29 인체 결장암세포에 대하여서는 85%aq. MeOH과 n-hexane의 분획물에서 월등히 높은 증식억제효과가 관찰되었는데, 이 억제효과는 인체 위암세포에서 보다도 높은 저해효과를 보여주었다. 이상의 결과로부터 해양생물 중 염생식물의 한 종류인 갯질경의 높은 항암활성효과와 함께 식용 및 약용으로의 가치가 있는 새로운 생리활성물질의 소재군으로서의 가능성을 확인할 수 있었다.

Keywords

References

  1. Shur, Y. J. (2004), Cnacer chemopreventive Effects of Dietrary Phytochemicals, J Korean Assoc Cancer Preve 9(2), 68-83
  2. Nagayama, K., Y. Iwamura, T. Shibata, I. Hirayama, and T. Nakamura (2002), Bactericidal activity of Phlorotannins from the brown alga Ecklonia Kurome, J Antimicrob Chemoth 50, 889-893 https://doi.org/10.1093/jac/dkf222
  3. Heo, S. J. and Y. J. Jeon (2005), Antioxidant effect and protecting effect against cell damage by enzymatic hydrolysates from marine algae, Food Industry Nutr 10, 31-41
  4. Kwak, C. S., S. A. Kim, and M. S. Lee (2005), The correlation of antioxidative effects of 5 Korean common edible seaweeds and total polyphenol content, J Korean Soc Food Sci Nutr 34, 1143-1150 https://doi.org/10.3746/jkfn.2005.34.8.1143
  5. Park, Y. B. (2005), Determination of nitrite-scavenging activity of seaweed, J Korean Soc Food Sci Nutr 34, 1293-1290
  6. Yuan, Y. V. and N. A. Walsh (2006), Antioxidant and antiproliferative activities of extracts from a variety of edible seaweeds, Food Chem Toxicol 44, 1144-1150 https://doi.org/10.1016/j.fct.2006.02.002
  7. Cho, K. J., Y. S. Lee, and B. H. Ryu (1990), Antitumor effect and immunology activity of seaweeds toward Sarcoma-180, J Korean Soc Food Sci Nutr 23, 345-352
  8. Lee, Y. S., D. S. Kim, B. H. Ryu, and S. H. Lee (1992), Antitumor and immunomodulating effects of seaweeds toward sarcoma-180 cell, J Korean Soc Food Sci Nutr 21, 544-550
  9. Park, S. Y., B. M. Jung, Y. H. Choi, and S. J. Bae (2005), Growth inhibition effects of cancer cell lines by gloiopeltis furcata fractions in vitro, J Korean Soc Food Sci Nutr 34, 771-775 https://doi.org/10.3746/jkfn.2005.34.6.771
  10. Ryu, B. H., D. S. Kim, K. J. Cho, and D. B. Sin (1989), Antitumor activity of seaweeds toward sarcoma-180, Korean J Food Sci Technol 21, 595-600
  11. Yan, X., Y. Chuda, M. Suzuki, and T. Nagata (1999), Fucoxanthin as the major antioxidant in hijikia fusiformis a common edible seaweed, Biosci Biotechnol Biochem 63(3), 603-607
  12. Bae, S. J. (2004), Anticarcinogen effects of Saorgassum fulvellum fractions on several human cancer cell lines in vitro, J Korean Soc Food Sci Nutr 33, 480-486 https://doi.org/10.3746/jkfn.2004.33.3.480
  13. Shine, M. O. and S. J. Bae (2005), Anti-proliferating effects of porphyra tenera fractions on several cancer cell lines in vitro, J Korean Soc Food Sci Nutr 34, 1543-1519
  14. Chas, S. H., G. N. Ahn, S. J. Heo, K. N. Kim, K. W. Lee, C. B. Song, S. K. Cho, and Y. J. Jeon, (2006), Screening of extracts from marine angiotensin- I converting enzyme (ACE) inhibitory activity, J Korean Soc Food Sci Nutr 35, 307-314 https://doi.org/10.3746/jkfn.2006.35.3.307
  15. Min, B. M. and J. H. Kim (1999), Plant community structure in reclaimed land on the West Coast of Korea, J Plant Biology 42, 287-293 https://doi.org/10.1007/BF03030342
  16. Shim, H. B, S. M. Seo, and B.H. Choi (2002), Floristic Survey of Salt Marshes and Dunes on Gyeonggi Bay in Korea, Korean J Environ Biol 20(1), 25-34
  17. Bang, M. A., H. A. Kim, and Y. J. Cho (2002), Hypolycemic and antioxidant effect of dietary hamcho powder in streptozotocin-induced Diabetic rats, J Korean Soc Food Sci Nutr 31(5), 840-846 https://doi.org/10.3746/jkfn.2002.31.5.840
  18. Seo, Y., H. J. Lee, Y. A. Kim, and K. E. Park (2004), Antioxidative effect of glasswort (Saliconia herbacea) extract from Daebudo, Proceeding of Current Biotechnology and Bioengineering 10, 1-7
  19. Han, S. K., S. M. Kim, and B. S. Pyo (2003), Antioxidative effect of glasswort (Salicornia herbacea L.) on the lipid oxidation of pork, Korean J Food Sci Ani Resour 23(1), 46-49
  20. Ng, T. B., F. Liu, and H. X. Wang (2004), The antioxidant effects of aqueous and organic extracts of Panax quinquefolium, Panax notoginseng, Codonopsis pilosula, Pseudostellaria heterophylla and Glehnia littoralis, J Ethnopharmacol 93, 285-288 https://doi.org/10.1016/j.jep.2004.03.040
  21. Kitamura, Y., M. Ohta, T. Ikenaga, and M. Watanabe (2002), Responses of anthocyanin-producing and non-producing cells of Glehnia littoralis to radical generators, Phytochem 59(1), 63-68 https://doi.org/10.1016/S0031-9422(01)00428-9
  22. Min, B. M. (1990), On the Accumulation of Minerals with the Plant Species in a Reclaimed Land, Korean J Ecol 13, 9-18
  23. Waisel, Y. (1972), Biology of Halophytes. Academic Press, London
  24. Kim, C. S. and T. G. Song (1983), Ecological Studies on the Halophyte Communities at Western and Southern Coasts in Korea(4)-The Halophyte Communities at the Different Salt Marsh Habitats, Korean J Ecol 6, 167-176
  25. Min, B. M. (1998), Vegetation on the West Coast of Korea, Ocean Research 20 Special, 167-178
  26. Park, J. G,, H. Frucht, R. V. LaRocca, D. P. Bliss, Y. Kurita, T. R. Chen, J. G. Henslee, J. B. Trepel, R. T. Jensen, B. E. Johnson, Y. J. Bang, J. P. Kim, and A. F. Gazdar (1990), Characterization of cell lines established from human gastric carcinoma, Cancer Res 50, 2773-2780
  27. Lee, B. H., Y. H. Moon, B. C. Jeong, K. S. Kim, and S. N. Ryu (2002), Growth characteristics and it's potentiality of use of halophyte, Suaeda asparagoides MIQ, Kor J Intl Agri 14(2), 87-93
  28. Im, S. A., G. W. Kim, and C. K. Lee (2003), Immunomodulatory activity of Salicornia herbacea L.components, Natural Product Science 9(4), 273-277
  29. Lee, K. S., M. H. Lee, I. Y. Chang, S. P. Yoon, D. Y. Lim, and Y. J. Jeon (2006), Marcrophage activation by polysaccharide fraction isolated from Salicornia herbacea, J Ethnopharmacol 103, 372-378 https://doi.org/10.1016/j.jep.2005.08.037