Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Transformation of Ginsenoside Rd to Ginsenoside F2 by Enzymes of Leuconostoc fallax LH3

Leuconostoc fallax LH3이 생산하는 효소에 의한 Ginsenoside Rd의 Ginsenoside F2로의 전환

  • Quan, Lin-Hu (Korean Ginseng Center for Most Valuable product and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Cheng, Le-Qin (Korean Ginseng Center for Most Valuable product and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Na, Ju-Ryun (Korean Ginseng Center for Most Valuable product and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Kim, Ho-Bin (Korean Ginseng Center for Most Valuable product and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Park, Min-Ju (Korean Ginseng Center for Most Valuable product and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Kim, Se-Hwa (Korean Ginseng Center for Most Valuable product and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Kim, Myung-Kyum (Korean Ginseng Center for Most Valuable product and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Yang, Deok-Chun (Korean Ginseng Center for Most Valuable product and Ginseng Genetic Resource Bank, Kyung Hee University)
  • 전림호 (경희대학교 고려인삼명품화 사업단 및 인삼유전자원소재은행) ;
  • 성락금 (경희대학교 고려인삼명품화 사업단 및 인삼유전자원소재은행) ;
  • 나주련 (경희대학교 고려인삼명품화 사업단 및 인삼유전자원소재은행) ;
  • 김호빈 (경희대학교 고려인삼명품화 사업단 및 인삼유전자원소재은행) ;
  • 박민주 (경희대학교 고려인삼명품화 사업단 및 인삼유전자원소재은행) ;
  • 김세화 (경희대학교 고려인삼명품화 사업단 및 인삼유전자원소재은행) ;
  • 김명겸 (경희대학교 고려인삼명품화 사업단 및 인삼유전자원소재은행) ;
  • 양덕춘 (경희대학교 고려인삼명품화 사업단 및 인삼유전자원소재은행)
  • Published : 2008.06.30
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Abstract

Ginsenosides have been regarded as the principal components, responsible for the pharmacological and biological activities of ginseng. Absorption of major ginsenosides at the gastrointestinal tract was extremely low, when ginseng taken orally. In order to improve the absorption and bioavailability, transformation of major ginsenosides into more active and valuable minor ginsenoside is much required. In this present study, We isolated a lactic acid bacteria Leuconostoc fallax LH3 from the Korean fermented food Kimchi, which have higher ${\beta}$-glucosidase activity. Using the ethanol precipitated curd enzyme of Leuconostoc fallax LH3, we investigated the biotransformation of ginsenoside Rd at different experimental condition to increase transformation. The maximum convertion was supported at 30 $^{\circ}C$ and decreased when temperatures increased. In order to optimize the effect of pH, the curd enzyme was mixed 20 mM sodium phosphate buffer (pH 3.5 to pH 8.0). Ginsenoside Rd was almost hydrolyzed between pH 7.0 and pH 9.0, but not hydrolyzed above pH 10.0. Ginsenoside Rd was hydrolyzed after 24 hrs incubation, but whereas the ginsenoside F2 was appeared from 36 hrs, and all ginsenoside Rd was transformed to F2 after the 60 hrs incubation. Based on this study, the curd enzyme of Leuconostoc fallax LH3 transformed the ginsenoside Rd at the 30$^{\circ}C$ and the pH optimum of 7.0 to 9.0 after the 60 hrs incubation time.

Keywords

References

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