Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
권호 표지

Free radical scavenging activity of hyangsapyungwisan extract for herbal-acupuncture and protective effects against oxidative damage of HUVECs

향사평위산(香砂平胃散) 약침액(藥鍼液)의 자유기 소거활성 및 혈관내피세포의 산화적 손상에 대한 보호효과

  • Lim, Sun-Hee (Department of Meridian & Acupoint, College of Oriental Medicine, Dongguk University) ;
  • Yi, Hyo-Seung (Department of Meridian & Acupoint, College of Oriental Medicine, Dongguk University) ;
  • Moon, Jin-Young (Department of Meridian & Acupoint, College of Oriental Medicine, Dongguk University)
  • 임선희 (동국대학교 한의과대학 경혈학교실) ;
  • 이효승 (동국대학교 한의과대학 경혈학교실) ;
  • 문진영 (동국대학교 한의과대학 경혈학교실)
  • Published : 2008.03.27
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Abstract

Objectives : Hyangsapyungwisan (HPS) has been used for treatment of cardiovascular diseases including of arthralgia, myalgia in traditional Korean medicine. However, the medical actions of HPS have not been clearly investigated. The aim of this study was to elucidate the antiradical and antioxidant activity of the extract for herb-acupuncture (HPS-HA) obtained from HPS. Methods & Results : HPS-HA exhibited a stronger inhibition rate (55.5%) on lipid peroxidation of rat liver homogenate induced by $FeCl_2$-ascorbic acid. In addition, HPS-HA markedly interfered with hydroxylradical generation through iron ions chelating action. While pro-oxidant effect of HPS-HA was nearly undetectable at concentrations of 0.5-10㎎/mL. Moerover, HPS-HA revealed a potent antiradical activities on 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radicals, superoxide anions, nitric oxide and peroxynitrite. Furthermore, HPS-HA inhibited copper- and AAPH-mediated oxidation of human low-density lipoprotein (LDL), and also suppressed the relative electrophoretic mobility of LDL. HPS-HA showed strong protective activity against oxidative damage of HUVECs induced by SIN-1. Conclusions : These results suggest that HPS-HA may be effective in protecting against oxidative diseases.

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