The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
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Effects of Astragalus membranaceus Extract on Cell Cycle and Apoptosis of Human Leiomyoma Cell

황기가 in vitro에서 자궁근종세포(子宮筋腫細胞)에 미치는 영향(影響)

  • 박준규 (경희대학교 한방 부인과교실) ;
  • 이진무 (경희대학교 한방 부인과교실) ;
  • 이창훈 (경희대학교 한방 부인과교실) ;
  • 조정훈 (경희대학교 한방 부인과교실) ;
  • 장준복 (경희대학교 한방 부인과교실) ;
  • 이경섭 (경희대학교 한방 부인과교실)
  • Published : 2008.05.30
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Abstract

Purpose: This study was conducted to investigate the effects of Astragalus membranaceus extract solution on cell cycle regulation and apoptosis of human leiomyomal cell. Methods: The leiomyoma cell of patients was used in the study, and we administered the extract solution of Astragalus membranaceus concentration at 1, $10mg/m{\ell}$ to the leiomyoma cell for 48 hours. We used flow cytometry and western blotting to confirm cell cycle and apoptosis. Results: In flow cytometry, G1 phase of the $1mg/m{\ell}$ and $10mg/m{\ell}$ group was shortened and S phase of the $1mg/m{\ell}$ and $10mg/m{\ell}$ group was increased. Cycline D1 expression increased in 1, $10mg/m{\ell}$ group. Bcl-2 expression decreased in 1, $10mg/m{\ell}$ groups than control group. And Bax expression that regulated cell apoptosis more increased in 1, $10mg/m{\ell}$ group than control group. VEGF expression rised in higher Astragalus membranaceus concentration group. Conclusion: This study suggest that Astragalus membranaceus might induce cell apoptosis of leiomyoma cell and shorten cell cycle. And Astragalus membranaceus would not have the regulation effect of cell cycle.

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