The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
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Proteomic Approach to Study the Antioxidant Activities of Dioscoreae Rhizoma on HeLa Cells

산약(山藥)의 항산화 작용에 대한 단백질체 분석 연구

  • Yang, Jeong-Min (Dept. of Ob&Gy, College of oriental Medicine, Donggguk University) ;
  • Lee, Ji-Hyung (Dept. of Biology. College of Science, Dongguk University) ;
  • Sung, Jung-Suk (Dept. of Biology. College of Science, Dongguk University) ;
  • Kim, Dong-Il (Dept. of Ob&Gy, College of oriental Medicine, Donggguk University)
  • 양정민 (동국대학교 한의과대학 부인과학교실) ;
  • 이지형 (동국대학교 자연과학대학 생물학과) ;
  • 성정석 (동국대학교 자연과학대학 생물학과) ;
  • 김동일 (동국대학교 한의과대학 부인과학교실)
  • Published : 2008.05.30
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Abstract

Purpose: This study was examined to verify the anti-oxidative effect of Dioscoreae Rhizoma on HeLa cells by proteomic approach. Methods: Aqueous extract was used to treat HeLa cell with different concentrations treated with water or MeOH extract of Dioscoreae Rhizoma. HeLa cells were co-treated with $H_2O_2$ and Dioscoreae Rhizoma extracts. Proteomics was done to identify, characterize, and quantitate proteins expressed in HeLa cells treated by $H_2O_2$ and Dioscoreae Rhizoma. Results: When HeLa cells were Co-treated with $H_2O_2$ and Dioscoreae batatas extracts, 16 proteins identified by 2-DE and MALDI-TOF mass spectrometry and database search. PRDX, HSP27 was major proteins of antioxidant effect by Dioscoreae batatas. Conclusion: Our results suggest that Dioscoreae Rhizoma extracts induce antioxidant effects by regulating proteins such as PRDX, HSP27.

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