The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
권호 표지

Application of Oral Acute Toxic Class Method and Globally Harmonized Classification System on Pesticide

국내에서 농약에 대한 급성경구독성등급법 적용 및 GHS 체계 도입 제안

  • Jeong, Mi-Hye (Department of Crop Life Safety, National Institute of Agricultural Science and Technology (NIAST), Rural Development Administration (RDA)) ;
  • You, Are-Sun (Department of Crop Life Safety, National Institute of Agricultural Science and Technology (NIAST), Rural Development Administration (RDA)) ;
  • Park, Kyung-Hun (Department of Crop Life Safety, National Institute of Agricultural Science and Technology (NIAST), Rural Development Administration (RDA)) ;
  • Kim, Byung-Seok (Department of Crop Life Safety, National Institute of Agricultural Science and Technology (NIAST), Rural Development Administration (RDA)) ;
  • Lee, Hee-Dong (Department of Crop Life Safety, National Institute of Agricultural Science and Technology (NIAST), Rural Development Administration (RDA)) ;
  • Lee, Jae-Bong (Department of Crop Life Safety, National Institute of Agricultural Science and Technology (NIAST), Rural Development Administration (RDA)) ;
  • Kwon, Oh-Kyung (Department of Crop Life Safety, National Institute of Agricultural Science and Technology (NIAST), Rural Development Administration (RDA))
  • 정미혜 (농업과학기술원 농산물안전성부) ;
  • 유아선 (농업과학기술원 농산물안전성부) ;
  • 박경훈 (농업과학기술원 농산물안전성부) ;
  • 김병석 (농업과학기술원 농산물안전성부) ;
  • 이희동 (농업과학기술원 농산물안전성부) ;
  • 이제봉 (농업과학기술원 농산물안전성부) ;
  • 권오경 (농업과학기술원 농산물안전성부)
  • Published : 2008.06.30
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Abstract

Globally Harmonized System (GHS) for classification and labelling will provide an internationally agreed hazard classification system of chemical products and for communication of those hazards. This study aimed for establishment of the oral acute toxic class (ATC) method and application of GHS on pesticides. The ATC method was developed for determining $LD_{50}$ estimates of chemical substances with significantly fewer animals than needed when applying conventional $LD_{50}$ tests. We carried out $LD_{50}$ test and ATC test on 13 pesticides, Although methidathion EC and parathion-ethyl EC showed significantly different in $LD_{50}$ values between $LD_{50}$ test and ATC method, there are no difference in toxicity class by GHS. Both tests on the other pesticides showed almost equal results and toxicity class by GHS. Therefore, this study indicated high possibility of application of ATC method and GHS on pesticides.

최근 국제적으로 화학물질에 대하여 분류체계를 단일화하기 위하여 "화학물질의 분류표지 등에 대한 세계 조화시스템(Globally Harmonized classification system, GHS)" 분류체계를 도입하고 있고, 또한 동물보호법에 따라 실험동물수를 축소한 급성경구독성시험법-고정용량법, 급성독성등급법(acute toxic class method, ATC), Up-and Down법을 새롭게 적용하고 있다. 본 시험은 국내 농약 등록시 수행하고 있는 급성경구독성시험인 $LD_{50}$ 시험법과 개정된 시험법 중 급성독성등급법(ATC)을 비교하여 새로운 시험법 적용과 GHS 분류체계 도입여부를 평가하기 위하여 실시하였다. 시험 농약은$LD_{50}$이 GHS 분류기준의 경계부분에 있는 농약 13종을 선발하여 $LD_{50}$ 시험법과 급성독성등급법을 동시에 수행한 결과 Methidathion EC, Parathion-ethyl의 $LD_{50}$은 $LD_{50}$ 시험법은 각각 57.3, $60\;mg\;kg^{-1}$ 이었고, 급성독성등급법은 $200\;mg\;kg^{-1}$ 으로 $LD_{50}$ 차이가 있었으나, GHS 분류체계의 독성구분 결과 모두 3급으로 동일하게 분류되었고, Carbofuran GR은 $LD_{50}$ 시험법은 $310\;mg\;kg^{-1}$, 급성독성등급법은 $300\;mg\;kg^{-1}$ 으로 비슷하였으나 GHS 분류 3급 기준이 $300\;mgvkg^{-1}$이하이므로 각각 4등급, 3등급으로 분류되었으며, Phosphamidon SL, Endosulfan EC, EPN EC, Dimethoate EC, Phenthoate EC, Zeta-cypermethrin EW, Bifenthrin EC, Fenthion EC, Carbaryl WP, Chlorpyrifos.Diflubenzuron WP의 $LD_{50}$ 은 두 시험 모두 비슷하였고, GHS 독성분류도 동일하였다. 이상의 결과 농약 등록시 급성경구독성시험에 급성독성등급법과 GHS 분류체계에 대한 적용가능성을 제시하고 있다.

Keywords

References

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